Anti-MX1 antibody [EPR19967]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MX1 antibody [EPR19967] (AB207414)

Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20U/mL IFN alpha 1 for 24h cells labeling MX1 with purified ab207414 at 1/500 dilution (1μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). Untreated control (Green).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR19967] (AB207414)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (Human Burkitt's lymphoma cell line) cells labeling MX1 with ab207414 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing negative staining on Daudi cell line. Expression was induced and showed cytoplasmic staining after cells were treated with IFN alpha 1 (20 U/ml) for 24h. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IP

Lab

Immunoprecipitation - Anti-MX1 antibody [EPR19967] (AB207414)

Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution

Lanes 1 - 2:

Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab207414 in Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

• WB

Lab

Western blot - Anti-MX1 antibody [EPR19967] (AB207414)

Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBST

Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-MX1 antibody [EPR19967] (ab207414) at 1/2000 dilution

Lane 1:

Untreated Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

• WB

Supplier Data

Western blot - Anti-MX1 antibody [EPR19967] (AB207414)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 seconds; Lane 2 : 1 minute.

All lanes:

Western blot - Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution

Lane 1:

Human spleen lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false