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Rabbit Monoclonal MX1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

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Images

Immunoprecipitation - Anti-MX1 antibody [EPR19967] (AB207414), expandable thumbnail
  • Western blot - Anti-MX1 antibody [EPR19967] (AB207414), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR19967] (AB207414), expandable thumbnail
  • Western blot - Anti-MX1 antibody [EPR19967] (AB207414), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MX1 antibody [EPR19967] (AB207414), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/40 - 1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.

Alternative names

Recommended products

Rabbit Monoclonal MX1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19967

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-MX1 antibody [EPR19967] (ab207414), expandable thumbnail

    Immunoprecipitation - Anti-MX1 antibody [EPR19967] (ab207414)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution

    Lanes 1 - 2: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 10 µg

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207414 in Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

  • Western blot - Anti-MX1 antibody [EPR19967] (ab207414), expandable thumbnail

    Western blot - Anti-MX1 antibody [EPR19967] (ab207414)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM /TBST

    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-MX1 antibody [EPR19967] (ab207414) at 1/2000 dilution

    Lane 1: Untreated Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 2: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR19967] (ab207414), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR19967] (ab207414)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (Human Burkitt's lymphoma cell line) cells labeling MX1 with ab207414 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing negative staining on Daudi cell line. Expression was induced and showed cytoplasmic staining after cells were treated with IFN alpha 1 (20 U/ml) for 24h. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-MX1 antibody [EPR19967] (ab207414), expandable thumbnail

    Western blot - Anti-MX1 antibody [EPR19967] (ab207414)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lane 2: 1 minute.

    All lanes: Western blot - Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution

    Lane 1: Human spleen lysate at 10 µg

    Lane 2: Human tonsil lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

  • Flow Cytometry (Intracellular) - Anti-MX1 antibody [EPR19967] (ab207414), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MX1 antibody [EPR19967] (ab207414)

    Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20U/mL IFN alpha 1 for 24h cells labeling MX1 with purified ab207414 at 1/500 dilution (1μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). Untreated control (Green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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