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Rabbit Recombinant Monoclonal MX1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.

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Images

Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (AB284604), expandable thumbnail
  • Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (AB284604), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (AB284604), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (AB284604), expandable thumbnail
  • Flow Cytometry - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (AB284604), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.

Alternative names

Interferon-induced GTP-binding protein Mx1, Interferon-induced protein p78, Interferon-regulated resistance GTP-binding protein MxA, Myxoma resistance protein 1, Myxovirus resistance protein 1, IFI-78K, MX1

Recommended products

Rabbit Recombinant Monoclonal MX1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR24485-19
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab284604 is the carrier-free version of Anti-MX1 antibody [EPR24485-19] ab284603.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration:

    15 seconds

    Exposure time:

    All lanes: Western blot - Anti-MX1 antibody [EPR24485-19] (Anti-MX1 antibody [EPR24485-19] ab284603) at 1/1000 dilution

    Lane 1: Untreated Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate at 20 µg

    Lane 2: Daudi treated with 20U/ml IFN alpha for 24 hours at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

  • Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 81 seconds

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MX1 antibody [EPR24485-19] (Anti-MX1 antibody [EPR24485-19] ab284603) at 1/1000 dilution

    All lanes: Human tonsil at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling MX1 with Anti-MX1 antibody [EPR24485-19] ab284603 at 1/500 (1.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with Anti-MX1 antibody [EPR24485-19] ab284603 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MX1 with Anti-MX1 antibody [EPR24485-19] ab284603 at 1/500 (1.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human tonsil. The section was incubated with Anti-MX1 antibody [EPR24485-19] ab284603 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Flow Cytometry - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) or untreated (Green) labelling MX1 with Anti-MX1 antibody [EPR24485-19] ab284603 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling MX1 with Anti-MX1 antibody [EPR24485-19] ab284603 at 1/500 (1.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human breast cancer (PMID:25838395). The section was incubated with Anti-MX1 antibody [EPR24485-19] ab284603 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labelling MX1 with Anti-MX1 antibody [EPR24485-19] ab284603 at 1/500 (1.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon cancer (PMID:24771638). The section was incubated with Anti-MX1 antibody [EPR24485-19] ab284603 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Daudi cells labelling MX1 with Anti-MX1 antibody [EPR24485-19] ab284603 at 1/250 (2.18 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in Daudi cell line, the signal increased after treatment with interferon alpha 1 (20 U/ml ) for 24 h. is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604), expandable thumbnail

    Western blot - Anti-MX1 antibody [EPR24485-19] - BSA and Azide free (ab284604)

    This data was developed using Anti-MX1 antibody [EPR24485-19] ab284603, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-MX1 antibody [EPR24485-19] (Anti-MX1 antibody [EPR24485-19] ab284603) at 1/1000 dilution

    Lane 1: His-tagged human Interferon-induced GTP-binding protein Mx1 recombinant protein (aa37-366) at 0.01 µg

    Lane 2: His-tagged human Interferon-induced GTP-binding protein Mx2 recombinant protein (aa86-412) at 0.01 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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