AB108525

Anti-MXD3/MAD3 antibody [EPR3882]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal MXD3/MAD3 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 4 publications.

View Alternative Names

BHLHC13, MAD3, MXD3, Max dimerization protein 3, Max dimerizer 3, Class C basic helix-loop-helix protein 13, Max-associated protein 3, Max-interacting transcriptional repressor MAD3, Myx, bHLHc13

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

All lanes:

Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3882

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity).

See full target information MXD3

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of genomics 2025:7355595 PubMed40406509

2025

Assessment of MXD3 Expression as a Predictor of Survival in Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhi Cao,Ning Zhang,Tangbing Chen,Hong Jiang

Cells 12: PubMed36831305

2023

Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ao,Zhuping Wu,Zhiwei Liao,Juncong Lan,Jie Zhang,Pengfei Sun,Baohua Liu,Zimei Wang

Cells 10: PubMed34943942

2021

MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wen Tsai,Kuo-Shyang Jeng,Mu-Kuang He,Yang-Wen Hsieh,Hsin-Hung Lai,Chi-Yu Lai,Chun-Chieh Huang,Chiung-Fang Chang,Chung-Tsui Huang,Guor Mour Her

Frontiers in molecular biosciences 8:702206 PubMed34859046

2021

MXD3 as an Immunological and Prognostic Factor From Pancancer Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhang,Xiaoqin He,Yue Li,Yangtao Xu,Wenliang Chen,Xin Liu,Xinyao Hu,Lin Xiong,Ximing Xu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MXD3/MAD3 antibody [EPR3882]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (AB108525)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (4)

Product promise