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Mouse Monoclonal MXI1 antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MXI1.

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Images

Immunocytochemistry - Anti-MXI1 antibody [PCRP-MXI1-1A3] (AB277094), expandable thumbnail
  • Flow Cytometry - Anti-MXI1 antibody [PCRP-MXI1-1A3] (AB277094), expandable thumbnail
  • Protein Array - Anti-MXI1 antibody [PCRP-MXI1-1A3] (AB277094), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human MXI1. Database link P50539

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytProtein Array
Human
Tested
Tested
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX.

Alternative names

Recommended products

Mouse Monoclonal MXI1 antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MXI1.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human MXI1. Database link P50539
Clone number
PCRP-MXI1-1A3
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from bioreactor concentrate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry - Anti-MXI1 antibody [PCRP-MXI1-1A3] (ab277094), expandable thumbnail

    Immunocytochemistry - Anti-MXI1 antibody [PCRP-MXI1-1A3] (ab277094)

    Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling MXI1 with ab277094 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

  • Flow Cytometry - Anti-MXI1 antibody [PCRP-MXI1-1A3] (ab277094), expandable thumbnail

    Flow Cytometry - Anti-MXI1 antibody [PCRP-MXI1-1A3] (ab277094)

    Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling MXI1 using ab277094 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

  • Protein Array - Anti-MXI1 antibody [PCRP-MXI1-1A3] (ab277094), expandable thumbnail

    Protein Array - Anti-MXI1 antibody [PCRP-MXI1-1A3] (ab277094)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277094.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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