Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle (PMID : 26109061).The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the human skeletal muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the mouse skeletal muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the rat skeletal muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cardiac muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cardiac muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

This data was developed using 308619, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST. The identities of the lower MW bands between 150 kDa and 37 kDa are expected to be MYBPC3 degradation products. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 1 second

All lanes:

Western blot - Anti-MYBPC3 antibody [EPR25673-58] (<a href='/en-us/products/primary-antibodies/mybpc3-antibody-epr25673-58-ab308619'>ab308619</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 140 kDa

Observed band size: 150 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620)

This data was developed using 308619, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : skeletal muscle (PMID : 23936073). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-MYBPC3 antibody [EPR25673-58] (<a href='/en-us/products/primary-antibodies/mybpc3-antibody-epr25673-58-ab308619'>ab308619</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 140 kDa

Observed band size: 150 kDa

false

Exposure time: 37s