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Rabbit Recombinant Monoclonal MYBPC3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (AB308620), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIP
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Tested
Tested
Not recommended

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Mouse

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Target data

Function

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Alternative names

Cardiac MyBP-C, MYBPC3

Recommended products

Rabbit Recombinant Monoclonal MYBPC3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25673-58

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling MYBPC3 with Anti-MYBPC3 antibody [EPR25673-58] ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the rat skeletal muscle.The section was incubated with Anti-MYBPC3 antibody [EPR25673-58] ab308619 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling MYBPC3 with Anti-MYBPC3 antibody [EPR25673-58] ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cardiac muscle.The section was incubated with Anti-MYBPC3 antibody [EPR25673-58] ab308619 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labelling MYBPC3 with Anti-MYBPC3 antibody [EPR25673-58] ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the mouse skeletal muscle.The section was incubated with Anti-MYBPC3 antibody [EPR25673-58] ab308619 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling MYBPC3 with Anti-MYBPC3 antibody [EPR25673-58] ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cardiac muscle.The section was incubated with Anti-MYBPC3 antibody [EPR25673-58] ab308619 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labelling MYBPC3 with Anti-MYBPC3 antibody [EPR25673-58] ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the human skeletal muscle.The section was incubated with Anti-MYBPC3 antibody [EPR25673-58] ab308619 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling MYBPC3 with Anti-MYBPC3 antibody [EPR25673-58] ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle (PMID: 26109061).The section was incubated with Anti-MYBPC3 antibody [EPR25673-58] ab308619 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Western blot - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    This data was developed using 308619, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The identities of the lower MW bands between 150 kDa and 37 kDa are expected to be MYBPC3 degradation products.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

    Exposure time: 1 second

    All lanes: Western blot - Anti-MYBPC3 antibody [EPR25673-58] (Anti-MYBPC3 antibody [EPR25673-58] ab308619) at 1/1000 dilution

    Lane 1: Human heart tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 140 kDa

    Observed band size: 150 kDa

    Exposure time: 1s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The identities of the lower MW bands between 150 kDa and 37 kDa are expected to be MYBPC3 degradation products.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

    Exposure time: 1 second

  • Western blot - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620), expandable thumbnail

    Western blot - Anti-MYBPC3 antibody [EPR25673-58] - BSA and Azide free (ab308620)

    This data was developed using 308619, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. Negative control: skeletal muscle (PMID: 23936073).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-MYBPC3 antibody [EPR25673-58] (Anti-MYBPC3 antibody [EPR25673-58] ab308619) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 3: Rat heart tissue lysate at 20 µg

    Lane 4: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 140 kDa

    Observed band size: 150 kDa

    Exposure time: 37s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. Negative control: skeletal muscle (PMID: 23936073).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

    Exposure time: 37 seconds

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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