AB133499

Anti-MYBPC3 antibody [EPR3009(2)]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal MYBPC3 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 6 publications.

View Alternative Names

Cardiac MyBP-C, MYBPC3

2 Images

Lab

Western blot - Anti-MYBPC3 antibody [EPR3009(2)] (AB133499)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYBPC3 antibody [EPR3009(2)] (ab133499) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 15 µg

Lane 2:

Mouse heart tissue lysate at 15 µg

Lane 3:

Rat heart tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 141 kDa

Observed band size: 150 kDa

false

Unknown

Western blot - Anti-MYBPC3 antibody [EPR3009(2)] (AB133499)

All lanes:

Western blot - Anti-MYBPC3 antibody [EPR3009(2)] (ab133499) at 1/1000 dilution

Lane 1:

Human heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 141 kDa

Observed band size: 150 kDa

false

Carrier free

Anti-MYBPC3 antibody [EPR3009(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3009(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

See full target information MYBPC3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 12:e70012 PubMed39169429

2024

Alterations in cardiac contractile and regulatory proteins contribute to age-related cardiac dysfunction in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Young Soo Han,Madona Pakkam,Matthew J Fogarty,Gary C Sieck,Frank V Brozovich

GeroScience 46:4543-4561 PubMed38656649

2024

Sex-specific cardiovascular remodeling leads to a divergent sex-dependent development of heart failure in aged hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Árpád Kovács,Saltanat Zhazykbayeva,Melissa Herwig,Gábor Á Fülöp,Tamás Csípő,Nikolett Oláh,Roua Hassoun,Heidi Budde,Hersh Osman,Mustafa Kaçmaz,Kornelia Jaquet,Dániel Priksz,Béla Juhász,Ibrahim Akin,Zoltán Papp,Wolfgang E Schmidt,Andreas Mügge,Ibrahim El-Battrawy,Attila Tóth,Nazha Hamdani

Life sciences 324:121712 PubMed37100378

2023

Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L Fitch,Raihan Kabir,Obialunanma V Ebenebe,Nicole Taube,Haley Garbus,Prithvi Sinha,Nadan Wang,Sumita Mishra,Brian L Lin,Grace K Muller,Mark J Kohr

Nature communications 13:3107 PubMed35661110

2022

Establishment of mouse model of inherited PIGO deficiency and therapeutic potential of AAV-based gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ryoko Kuwayama,Keiichiro Suzuki,Jun Nakamura,Emi Aizawa,Yoshichika Yoshioka,Masahito Ikawa,Shin Nabatame,Ken-Ichi Inoue,Yoshiari Shimmyo,Keiichi Ozono,Taroh Kinoshita,Yoshiko Murakami

Frontiers in cellular and infection microbiology 11:689707 PubMed34621686

2021

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Charman,Steven McFarlane,Joanna K Wojtus,Elizabeth Sloan,Rebecca Dewar,Gail Leeming,Mohammed Al-Saadi,Laura Hunter,Miles W Carroll,James P Stewart,Paul Digard,Edward Hutchinson,Chris Boutell

Journal of cellular physiology 235:7870-7888 PubMed31943169

2020

Functional characterization of human myosin-binding protein C3 variants associated with hypertrophic cardiomyopathy reveals exon-specific cardiac phenotypes in zebrafish model.

Applications

Unspecified application

Species

Unspecified reactive species

Sahar I Da'as,Huseyin C Yalcin,Gheyath K Nasrallah,Iman A Mohamed,Michail Nomikos,Magdi H Yacoub,Khalid A Fakhro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYBPC3 antibody [EPR3009(2)]

Western blot - Anti-MYBPC3 antibody [EPR3009(2)] (AB133499)

Western blot - Anti-MYBPC3 antibody [EPR3009(2)] (AB133499)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

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