Mouse Monoclonal Myc tag antibody. Carrier free. Suitable for IP, Flow Cyt, ELISA, Purification, ICC/IF, IHC-Fr, WB and reacts with Tag samples.
Images
![Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (AB264603), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e10-bsa-and-azide-free-ab264603--western-blot-img170340.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (AB264603)")
![Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (AB264603), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e10-bsa-and-azide-free-ab264603--western-blot-img117300.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (AB264603)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ELISA | Purification | ICC/IF | IHC-Fr | WB | |
|---|---|---|---|---|---|---|---|
| Tag | Expected | Expected | Expected | Expected | Tested | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Purification
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes See Abreviews. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Version
14 products for Alternative Product
Target data
Alternative names
Myc Epitope Tag, c-myc tag
Recommended products
Mouse Monoclonal Myc tag antibody. Carrier free. Suitable for IP, Flow Cyt, ELISA, Purification, ICC/IF, IHC-Fr, WB and reacts with Tag samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 9E10
- Purity
- IgG fraction
- Epitope
- aa 410-419 of human Myc.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab264603 is the carrier-free version of Anti-Myc tag antibody [9E10] ab32.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e10-bsa-and-azide-free-ab264603--western-blot-img170340.jpg "Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)")
Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
False colour image of Western blot: Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Myc tag antibody [9E10] ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line Human MYC (c-Myc) knockout HEK-293T cell line ab256500 (CRISPR-Cas9 edited cell lysate Human MYC (c-Myc) knockout HEK-293T cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
Lanes 1 - 4: Western blot - Anti-Myc tag antibody [9E10] (Anti-Myc tag antibody [9E10] ab32) at 1/200 dilution
Lanes 1 - 4: Western blot - Anti-Myc tag antibody [9E10] (Anti-Myc tag antibody [9E10] ab206486) at 1/200 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: MYC CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (Human MYC (c-Myc) knockout HEK-293T cell line ab256500)
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 48 kDa, 49 kDa
![Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e10-bsa-and-azide-free-ab264603--western-blot-img117300.jpg "Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)")
Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide and Arginine (Anti-Myc tag antibody [9E10] ab32).
Lysate from E. coli recombinantly expressing 11 commonly used tags including myc tag.
All lanes: Western blot - Anti-Myc tag antibody [9E10] (Anti-Myc tag antibody [9E10] ab32) at 1 µg/mL
All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (E. coli Positive Control (Escherichia coli ) Whole Cell Lysate ab5395) at 10 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa, 49 kDa
Observed band size: 45 kDa
Exposure time: 1min
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