Mouse Monoclonal Myc tag antibody. Carrier free. Suitable for IP, WB and reacts with Tag - Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (AB264433), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e11-bsa-and-azide-free-ab264433--immunoprecipitation-img21704.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (AB264433)")
![Flow Cytometry (Intracellular) - Anti-Myc tag antibody [9E11] - BSA and Azide free (AB264433), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e11-bsa-and-azide-free-ab264433--flow-cytometry-intracellular-img57545.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Myc tag antibody [9E11] - BSA and Azide free (AB264433)")
Publications
Filter by
- Nature communications 11:7252020Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Lyu et. al.PubMed 32024833
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | WB | |
|---|---|---|---|
| Synthetic peptide | Not recommended | Not recommended | Not recommended |
| Tag - Human | Tested | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Tag - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag - Human | Dilution info - | Notes Additional non-specific bands observed at 75, 110, 140 kDa using mouse and human cells (see Abreview). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
1 products for Alternative Version
Target data
Alternative names
Myc Epitope Tag, c-myc tag
Recommended products
Mouse Monoclonal Myc tag antibody. Carrier free. Suitable for IP, WB and reacts with Tag - Human samples. Cited in 1 publication.
Alternative names
Myc Epitope Tag, c-myc tag
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
9E11
- Purification technique
Affinity purification Immunogen
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab264433 is the carrier-free version of Anti-Myc tag antibody [9E11] ab56.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e11-bsa-and-azide-free-ab264433--immunoprecipitation-img21704.jpg "Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)")
Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)
This image was produced using the same antibody clone in a different formulation Anti-Myc tag antibody [9E11] ab56.
Myc tag was immunoprecipitated using 0.5mg CHO overexpressing Stra8 whole cell lysate, 5μg of Mouse monoclonal to Myc tag and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, CHO overexpressing Stra8 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-Myc tag antibody [9E11] ab56.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 49KDa; Myc tag
All lanes: Immunoprecipitation - Anti-Myc tag antibody [9E11] (Anti-Myc tag antibody [9E11] ab56)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 20min
![Flow Cytometry (Intracellular) - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-9e11-bsa-and-azide-free-ab264433--flow-cytometry-intracellular-img57545.jpg "Flow Cytometry (Intracellular) - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)")
Flow Cytometry (Intracellular) - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)
This image was produced using the same antibody clone in a different formulation Anti-Myc tag antibody [9E11] ab56.
Overlay histogram showing HL60 cells stained with Anti-Myc tag antibody [9E11] ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Myc tag antibody [9E11] ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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