Rabbit Recombinant Monoclonal C-Myc/MYC antibody. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MYC aa 400 to C-terminus.
Images
![Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (AB314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--immunocytochemistry-img420693.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (AB314108)")
![Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (AB314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--immunocytochemistry-img420692.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (AB314108)")
![Immunoprecipitation - Anti-Myc Tag antibody [BLRE01G] (AB314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--immunoprecipitation-img420691.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Myc Tag antibody [BLRE01G] (AB314108)")
![Western blot - Anti-Myc Tag antibody [BLRE01G] (AB314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--western-blot-img420690.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myc Tag antibody [BLRE01G] (AB314108)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01106
Reactivity data
Select an application| ICC | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Alternative names
BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39
Recommended products
Rabbit Recombinant Monoclonal C-Myc/MYC antibody. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MYC aa 400 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01106
- Clone number
BLRE01G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
Up to 12 months
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (ab314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--immunocytochemistry-img420693.jpg "Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (ab314108)")
Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (ab314108)
Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged cytoplasmic protein by ICC. Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (A191-101). Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.
![Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (ab314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--immunocytochemistry-img420692.jpg "Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (ab314108)")
Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (ab314108)
Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged nuclear protein by ICC. Antibody: Rabbit antiMyc Tag recombinant monoclonal antibody [BLRE01G] (A191-101). Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.
![Immunoprecipitation - Anti-Myc Tag antibody [BLRE01G] (ab314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--immunoprecipitation-img420691.jpg "Immunoprecipitation - Anti-Myc Tag antibody [BLRE01G] (ab314108)")
Immunoprecipitation - Anti-Myc Tag antibody [BLRE01G] (ab314108)
Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (A191-101) used for IP at 20 µl/mg lysate. For blotting immunoprecipitated Myc Tag, A191-101 was used at 1:1000. Chemiluminescence with an exposure time of 1 seconds.
![Western blot - Anti-Myc Tag antibody [BLRE01G] (ab314108), expandable thumbnail](https://content.abcam.com/products/images/myc-tag-antibody-blre01g-ab314108--western-blot-img420690.jpg "Western blot - Anti-Myc Tag antibody [BLRE01G] (ab314108)")
Western blot - Anti-Myc Tag antibody [BLRE01G] (ab314108)
Antibody:Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (A191-101 lot 1) used at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.
All lanes: Western blot - Anti-Myc Tag antibody [BLRE01G] (ab314108) at 1/1000 dilution
Lane 1: non-transfected HEK293 lysate
Lane 2: HEK293 transfected with Met-Myc-IDO1 lysate
Lane 3: HEK293 transfected with IDO1-Myc (C-terminal Tag) lysate
Lane 4: HEK293 transfected with IDO1-Myc-HIS (Internal Tag) lysate
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Downloads
Product protocols
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