AB314108

Anti-Myc Tag antibody [BLRE01G]

RabMAb
Recombinant
KO Validated
Lab Essentials
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Rabbit Recombinant Monoclonal C-Myc/MYC antibody. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MYC aa 400 to C-terminus.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

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Supplier Data

Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (AB314108)

Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged cytoplasmic protein by ICC. Antibody : Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (A191-101). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-Myc Tag antibody [BLRE01G] (AB314108)

Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged nuclear protein by ICC. Antibody : Rabbit antiMyc Tag recombinant monoclonal antibody [BLRE01G] (A191-101). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-Myc Tag antibody [BLRE01G] (AB314108)

Antibody : Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (A191-101) used for IP at 20 µl/mg lysate. For blotting immunoprecipitated Myc Tag, A191-101 was used at 1 : 1000. Chemiluminescence with an exposure time of 1 seconds.

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Supplier Data

Western blot - Anti-Myc Tag antibody [BLRE01G] (AB314108)

Antibody : Rabbit anti-Myc Tag recombinant monoclonal antibody [BLRE01G] (A191-101 lot 1) used at 1 : 1000. Secondary : HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-Myc Tag antibody [BLRE01G] (ab314108) at 1/1000 dilution

Lane 1:

non-transfected HEK293 lysate

Lane 2:

HEK293 transfected with Met-Myc-IDO1 lysate

Lane 3:

HEK293 transfected with IDO1-Myc (C-terminal Tag) lysate

Lane 4:

HEK293 transfected with IDO1-Myc-HIS (Internal Tag) lysate

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLRE01G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IP

applications

Immunogen

Synthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01106

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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You may be interested in:

AB277452

Human c-Myc ELISA Kit

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com