AB9132

Anti-Myc tag antibody - ChIP Grade

(7 Reviews)

(171 Publications)

Goat Polyclonal Myc tag antibody. Suitable for ICC, IP, ChIP, ELISA, WB and reacts with Tag samples. Cited in 171 publications.

2 Images

ChIP - Anti-Myc tag antibody - ChIP Grade (AB9132)

ChIP

Unknown

ChIP - Anti-Myc tag antibody - ChIP Grade (AB9132)

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

Supplier Data

Western blot - Anti-Myc tag antibody - ChIP Grade (AB9132)

All lanes:

Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/mL

Lane 1:

E. coli whole cell lysate at 0.2 µg

Lane 2:

E. coli whole cell lysate at 0.1 µg

Lane 3:

E. coli whole cell lysate at 0.05 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

WB, ELISA, ICC, ChIP, IP

applications

Specificity

Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "Also PubMed: 20224777", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Myc tag

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Publications (171)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:422 PubMed40998798

2025

TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xintian Zhang,Han Yao,Yichao Hou,Kun Zhou,Yu Liang,Lidan Hou,Xingming Zhang,Wenfeng Wang,Leilei Du,Mengfei Yao,Jianhua Wang,Xiangjun Meng

Oncology letters 30:446 PubMed40740985

2025

circEFR3A promotes breast cancer progression by sponging miR-590-3p to upregulate androgen receptor expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhe Mi,Xinle Wang,Han Song,Zhenyu Wu,Sainan Li,Fei Liu,Wei Liu,Meixiang Sang,Cuizhi Geng

Development (Cambridge, England) 152: PubMed40326503

2025

The Rho GEF Trio functions in contact inhibition of locomotion of neural crest cells by interacting with Ptk7.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Till,Annette Borchers

Nature 637:496-503 PubMed39633052

2024

Mis-splicing of a neuronal microexon promotes CPEB4 aggregation in ASD.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Garcia-Cabau,Anna Bartomeu,Giulio Tesei,Kai Chit Cheung,Julia Pose-Utrilla,Sara Picó,Andreea Balaceanu,Berta Duran-Arqué,Marcos Fernández-Alfara,Judit Martín,Cesare De Pace,Lorena Ruiz-Pérez,Jesús García,Giuseppe Battaglia,José J Lucas,Rubén Hervás,Kresten Lindorff-Larsen,Raúl Méndez,Xavier Salvatella

Signal transduction and targeted therapy 9:337 PubMed39617783

2024

Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Lin,Ling Li,Bin Yuan,Fei Luo,Xiujuan Zhang,Yuanjun Yang,Shaliu Luo,Jing Lin,Tianxing Ye,Youzhi Zhang,Shan Gao,Qinong Ye

Molecular biology of the cell 36:ar1 PubMed39565681

2024

A defined tubby domain β-barrel surface region of TULP3 mediates ciliary trafficking of diverse cargoes.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Reddy Palicharla,Hemant B Badgandi,Sun-Hee Hwang,Emilie Legué,Karel F Liem,Saikat Mukhopadhyay

The Plant journal : for cell and molecular biology 119:1558-1569 PubMed38865085

2024

Integration of C3H15-mediated transcriptional and post-transcriptional regulation confers plant thermotolerance in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Guohua Chai,Huanhuan Liu,Yang Zhang,Congpeng Wang,Hua Xu,Guo He,Jie Meng,Xianfeng Tang,Dian Wang,Gongke Zhou

iScience 27:109768 PubMed38711441

2024

Molecular clues unveiling spinocerebellar ataxia type-12 pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Kumar,Shweta Sahni,Vivekanand A,Deepak Kumar,Neetu Kushwah,Divya Goel,Himanshi Kapoor,Achal K Srivastava,Mohammed Faruq

eLife 13: PubMed38289024

2024

Heat stress-induced activation of MAPK pathway attenuates Atf1-dependent epigenetic inheritance of heterochromatin in fission yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Libo Liu,Chunlin Song,Yamei Wang,Quan-Wen Jin

Cells 13: PubMed38247840

2024

Is Involved in Congenital Chylothorax.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan B Whitchurch,Sophia Schneider,Alina C Hilger,Ricarda Köllges,Jil D Stegmann,Lea Waffenschmidt,Laura Dyer,Holger Thiele,Bhanupriya Dhabhai,Tikam Chand Dakal,Andreas Müller,Dominic P Norris,Heiko M Reutter

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myc tag antibody - ChIP Grade

ChIP - Anti-Myc tag antibody - ChIP Grade (AB9132)

Western blot - Anti-Myc tag antibody - ChIP Grade (AB9132)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (171)

Product promise