Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric)

5 product images

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  Immunocytochemistry - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells transfected with CRISPR-Cas9 (S. Aureas) expression vector containing a myc tag, labeling myc-tag with ab289980 at 1/500 (2.202 μg/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with CRISPR-Cas9 (S. Aureas) expression vector containing a myc tag.
  

  Nulcear counterstain is DAPI. Cells were also counterstained with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 Anti-CRISPR-Cas9 rabbit monoclonal antibody at 1/500 (2 μg/ml) dilution, followed by secondary antibody Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 Anti-CRISPR-Cas9 rabbit monoclonal antibody at 1/200 (2.5 μg/ml) dilution.

  -ve control 1: ab289980 at 1/500 (2.202 μg/ml) dilution, followed by ab15088 1/200 (2.5 μg/ml)

  -ve control 2: ab15088 1/200 (2 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1/1000 (2 μg/ml)

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  Western blot - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980)

  Blocking and dilution buffer: 5% NFDM/TBST
  

  ab289980 detects a ranged of myc tagged proteins in WB.

  All lanes: Western blot - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980) at 1/5000 dilution

  Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 1 µg

  Lane 2: HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

  Lane 3: HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

  Lane 4: HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

  Lane 5: HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

  Lane 6: HEK-293T transfected with human Alphasynuclein expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

  Secondary

  All lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (Goat Anti-Mouse IgG Fc (HRP) preadsorbed ab98717) at 1/10000 dilution

  Predicted band size: 49 kDa

  Exposure time: 3s

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  Immunoprecipitation - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980)

  Myc-tag was immunoprecipitated from HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate (2μg). Western blot was performed using immunopreciptate, ab289980 was used at 1/30 dilution (2μg in 0.35mg lysates). Secondary antibody used was Veriblot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution.
  

  Lane 1 (Input): HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate, 2μg.

  Lane 2 (+): HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate

  Lane 3 (-): Human IgG instead of ab289980 in HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate

  Blocking and dilution buffer: 5% NFDM/TBST.

  ab289980 detects a ranged of myc tagged proteins in WB.

  Lane 1: Immunoprecipitation - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980)

  Lane 1: Immunoprecipitation - Anti-Myc tag antibody [Hyper-myc] (Anti-Myc tag antibody [Hyper-myc] ab289981)

  Predicted band size: 49 kDa

  Observed band size: 70 kDa

  Exposure time: 6s

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  Flow Cytometry (Intracellular) - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980)

  Intracellular flow cytometric analysis of 293T (human embryonic kidney) transfected with TMEM-119 expression vector containing a myc-His-tag®, fixed with 2% paraformaldehyde and permeabelized 0.1% Tween-20. Cells were surface stained with TMEM-119 conjugated with Alexa Fluor® 647 (Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker ab225494). Then fixed with 2% PFA for 10min followed by intracellularly stained with mouse IgG or ab289980. ab289980 was used at 1:1000 dilution (0.1μg)(Right) compared with Mouse monoclonal IgG (Left). Secondary antibody used was Goat anti-Mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution.

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  ELISA - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (ab289980)

  Indirect ELISA antibody dose-response curve of ab289980 for Human myc-tag antigen, at a concentration of 1000ng/ml. Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution.