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AB51804

Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46]

5

(1 Review)

|

(1 Publication)

Mouse Monoclonal DHA antibody. Suitable for ELISA, WB and reacts with Mycobacterium tuberculosis samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Mycobacterium L-alanine dehydrogenase protein.

View Alternative Names

Rv2780, MTV002.45, ald, Alanine dehydrogenase, 40 kDa antigen, TB43

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WHO no. IT-46

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mycobacterium tuberculosis

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mycobacterium tuberculosis": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mycobacterium L-alanine dehydrogenase also known as AlaDH is an enzyme that catalyzes the reversible oxidative deamination of L-alanine to pyruvate and ammonia. This reaction involves NAD+ as a coenzyme. The protein has a molecular mass of approximately 46 kDa. AlaDH is expressed in Mycobacterium species including Mycobacterium tuberculosis. It plays an important role in the metabolic processes of these bacteria particularly in maintaining nitrogen balance.
Biological function summary

L-alanine dehydrogenase participates in nitrogen metabolism and is essential for the survival of Mycobacterium in diverse environments. It does not form part of a larger protein complex but functions independently to facilitate the conversion of amino acids. The activity of AlaDH aids in providing energy and building blocks necessary for cell survival especially when the bacteria are under stress or nutrient-limiting conditions.

Pathways

L-alanine dehydrogenase plays an important role in the alanine and aspartate metabolism pathways. These pathways are critical for energy production and biosynthesis in Mycobacterium species. AlaDH connects closely with other enzymes in the amino acid metabolism network influencing processes such as gluconeogenesis and nitrogen assimilation. Its activity affects the levels of related metabolites impacting the balance of these interconnected metabolic pathways.

L-alanine dehydrogenase has relevance in the study of tuberculosis. The enzyme's function and expression can influence the pathogenicity of Mycobacterium tuberculosis. AlaDH may interact with other proteins involved in Mycobacterium virulence making it a potential target for anti-tuberculosis drugs. Researchers investigate its role in mycobacterial persistence within human hosts contributing to the understanding of chronic infection dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible reductive amination of pyruvate to L-alanine. However, since the physiological environment of M.tuberculosis has a neutral pH, it can be assumed that the enzyme catalyzes exclusively the formation of L-alanine. May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.
See full target information ald
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gene 212:21-9 PubMed9661660

1998

Host vector system for high-level expression and purification of recombinant, enzymatically active alanine dehydrogenase of Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

B Hutter,M Singh
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