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AB36731

Anti-Mycobacterium tuberculosis Ag85 antibody [HYT27]

2

(2 Reviews)

|

(10 Publications)

Mouse Monoclonal TB Ag85A antibody. Suitable for ELISA, WB and reacts with Mycobacterium tuberculosis samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing Mycobacterium tuberculosis Ag85 protein.

View Alternative Names

mpt44, Rv3804c, MTV026.09c, fbpA, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex A, Fibronectin-binding protein A, 85A, Ag85A, Fbps A

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HYT27

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mycobacterium tuberculosis

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Epitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).

Specificity

This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1/400", "ELISA-species-notes": "<p>Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mycobacterium tuberculosis": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/400", "ELISA-species-notes": "<p>Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

WHO no. IT-49
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Mycobacterium tuberculosis Ag85 also called Ag85 complex or Ag-85 consists of a series of related proteins secreted by Mycobacterium tuberculosis including Ag85A Ag85B and Ag85C. This protein family plays a central role in the pathogen's ability to synthesize mycolic acids. The Ag85 complex has a molecular mass of around 30 to 32 kDa and is abundantly expressed on the surface of the Mycobacterium tuberculosis cell wall.
Biological function summary

The Ag85 complex facilitates the synthesis and transfer of mycolic acids essential constituents of the mycobacterial cell wall's lipid-rich structure. The Ag85 proteins participate in forming this barrier allowing Mycobacterium tuberculosis to resist host immune defenses. This functionality is linked to its involvement in the mycolyltransferase enzyme system which operates in collaboration with several other proteins necessary for full cell wall biosynthesis.

Pathways

The Ag85 complex significantly contributes to the biosynthetic route of mycolic acids an integral component of mycobacterial cell walls. This pathway includes interactions with other proteins like Pks13 and Mycobacterial arabinosyltransferase (EmbB) both important for the complex architectural integrity and viability of the mycobacterial cell wall. This biosynthesis pathway ensures proper assembly and maintenance of the cell envelope enhancing the persistence of the bacteria.

The Ag85 proteins are critical in the pathogenesis of tuberculosis where their activity supports the survival of the bacteria within host macrophages. Ag85 complexes are considered potential targets for diagnostic and therapeutic applications against tuberculosis. Additionally in leprosy another disease caused by mycobacteria proteins of the Ag85 complex have shown cross-reactivity emphasizing their significant role in bacterial viability and immune response evasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26 : 0-CoA over C18 : 1-CoA.
See full target information fbpA

Additional targets

fbpB,fbpC
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 8:803-818 PubMed36959508

2023

ATG7 and ATG14 restrict cytosolic and phagosomal Mycobacterium tuberculosis replication in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Beren Aylan,Elliott M Bernard,Enrica Pellegrino,Laure Botella,Antony Fearns,Natalia Athanasiadi,Claudio Bussi,Pierre Santucci,Maximiliano G Gutierrez

Nature communications 14:1430 PubMed36932056

2023

A mycobacterial effector promotes ferroptosis-dependent pathogenicity and dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Qiang,Yong Zhang,Zehui Lei,Zhe Lu,Shasha Tan,Pupu Ge,Qiyao Chai,Mengyuan Zhao,Xinwen Zhang,Bingxi Li,Yu Pang,Lingqiang Zhang,Cui Hua Liu,Jing Wang

Advanced healthcare materials 11:e2200589 PubMed35678244

2022

Biofabrication of Multiplexed Electrochemical Immunosensors for Simultaneous Detection of Clinical Biomarkers in Complex Fluids.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjay S Timilsina,Mohanraj Ramasamy,Nolan Durr,Rushdy Ahmad,Pawan Jolly,Donald E Ingber

Molecular medicine reports 24: PubMed34558650

2021

Exosomes in serum‑free cultures of THP‑1 macrophages infected with .

Applications

Unspecified application

Species

Unspecified reactive species

Fantahun Biadglegne,Phil Rademacher,Yarúa Gabriela Jaimes De Sulbaran,Brigitte König,Arne C Rodloff,Ulrike Zedler,Anca Dorhoi,Ulrich Sack

PLoS pathogens 17:e1009410 PubMed33720986

2021

Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation.

Applications

Unspecified application

Species

Unspecified reactive species

Christophe J Queval,Antony Fearns,Laure Botella,Alicia Smyth,Laura Schnettger,Morgane Mitermite,Esen Wooff,Bernardo Villarreal-Ramos,Waldo Garcia-Jimenez,Tiaan Heunis,Matthias Trost,Dirk Werling,Francisco J Salguero,Stephen V Gordon,Maximiliano G Gutierrez

Nature communications 10:1973 PubMed31036822

2019

A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyao Chai,Xudong Wang,Lihua Qiang,Yong Zhang,Pupu Ge,Zhe Lu,Yanzhao Zhong,Bingxi Li,Jing Wang,Lingqiang Zhang,Dawang Zhou,Wei Li,Wenzhu Dong,Yu Pang,George Fu Gao,Cui Hua Liu

PLoS pathogens 10:e1004183 PubMed24874799

2014

The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis.

Applications

WB

Species

Unspecified reactive species

Luis Solans,Jesús Gonzalo-Asensio,Claudia Sala,Andrej Benjak,Swapna Uplekar,Jacques Rougemont,Christophe Guilhot,Wladimir Malaga,Carlos Martín,Stewart T Cole

Proceedings of the National Academy of Sciences of 105:264-9 PubMed18172199

2008

Mycobacterium tuberculosis Rv2224c modulates innate immune responses.

Applications

WB

Species

Unspecified reactive species

Jyothi Rengarajan,Elissa Murphy,Arnold Park,Cassandra L Krone,Erik C Hett,Barry R Bloom,Laurie H Glimcher,Eric J Rubin

Infection and immunity 55:2922-7 PubMed3119491

1987

Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs.

Applications

Unspecified application

Species

Unspecified reactive species

A Worsaae,L Ljungqvist,K Hasløv,I Heron,J Bennedsen

Journal of clinical microbiology 23:446-51 PubMed2420818

1986

Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay.

Applications

Unspecified application

Species

Unspecified reactive species

A B Andersen,Z L Yuan,K Hasløv,B Vergmann,J Bennedsen
View all publications
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