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AB43019

Anti-Mycobacterium tuberculosis Ag85B antibody

4

(4 Reviews)

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(21 Publications)

Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) is a rabbit polyclonal antibody detecting Mycobacterium tuberculosis Ag85B in Western Blot, IHC-P, ELISA.

- Over 10 publications
- Trusted since 2006
2 Images
Western blot - Anti-Mycobacterium tuberculosis Ag85B antibody (AB43019)
  • WB

AbReview8117****

Western blot - Anti-Mycobacterium tuberculosis Ag85B antibody (AB43019)

All lanes:

Western blot - Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) at 1/5000 dilution

All lanes:

E coli, expressing his-tagged Ag85b

Secondary

Lane 1:

HRP conjugated goat anti-rabbit IgG

Lane 2:

HRP conjugated goat anti-rat IgG

Predicted band size: 34 kDa

Observed band size: 28 kDa,34 kDa

false

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mycobacterium tuberculosis Ag85B antibody (AB43019)
  • IHC-P

AbReview35377****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mycobacterium tuberculosis Ag85B antibody (AB43019)

ab17976 staining Mycobacterium tuberculosis Ag85B in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 25°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mycobacterium tuberculosis

Applications

IHC-P, WB, ELISA

applications

Specificity

This antibody is specific for the Ag85B protein in sera, plasma or cell culture supernatant.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA. What is the molecular weight of Mycobacterium tuberculosis Ag85B?
Anti-Mycobacterium tuberculosis Ag85B (ab43019) specifically detects a band for Mycobacterium tuberculosis Ag85B (UniProt: P9WQP1) at a molecular weight of 34kDa.

Trusted by the scientific community
Anti-Mycobacterium tuberculosis Ag85B (ab43019) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 1.2114% Sodium citrate, 0.6057% Tris, 0.05% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mycobacterium tuberculosis Ag85B protein also known as Antigen 85B or FBPA is a component of the Ag85 complex. This protein has a molecular mass of approximately 31 kDa. The Ag85B protein plays an important mechanical role by catalyzing the formation of the cord factor a major glycolipid in the cell wall of Mycobacterium tuberculosis. It is expressed in the mycobacterium's cell wall contributing to the virulence of this pathogen by promoting the invasion and survival within the host.
Biological function summary

The Ag85B protein serves in the assembly and maintenance of the mycobacterial cell wall by synthesizing mycolate. It is a part of the Ag85 complex which includes Ag85A Ag85B and Ag85C ensuring accurate synthesis and export of essential components of the cell wall. Through its actions Ag85B is involved in interacting with the host immune system and is a target for vaccine development due to its immunogenic properties. Its presence is detected during histological examinations of infected tissues where it shows the interaction with host cells.

Pathways

The Ag85B protein functions within the mycolic acid biosynthesis pathway essential for maintaining the structure of the mycobacterial cell envelope. This protein interacts with Ag85A and Ag85C to orchestrate complex lipid synthesis required for Mycobacterium tuberculosis' survival. Ag85B's role in lipid metabolism makes it important in the pathogen's adaptation to the host environment by altering the cell wall to evade immune detection.

Ag85B is highly relevant to respiratory diseases like tuberculosis. This protein plays a role in the pathogen's ability to resist destruction by the host's immune system contributing to the chronic nature of tuberculosis. Additionally Ag85B has been investigated in the context of its potential utility in vaccines and diagnostic tests for Mycobacterium tuberculosis infections. Its interactions with other proteins in the Ag85 complex make it a focus of research for developing therapeutics to treat and manage tuberculosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Ag85B
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 105:105196 PubMed38880068

2024

Spatial distribution of Mycobacterium tuberculosis mRNA and secreted antigens in acid-fast negative human antemortem and resected tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Kievershen Nargan,Joel N Glasgow,Sajid Nadeem,Threnesan Naidoo,Gordon Wells,Robert L Hunter,Anneka Hutton,Kapongo Lumamba,Mpumelelo Msimang,Paul V Benson,Adrie J C Steyn

Microbiology spectrum 12:e0412623 PubMed38700329

2024

Genomic and phenotypic characterization of closest-related non-tuberculous mycobacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Sous,Wafa Frigui,Alexandre Pawlik,Fadel Sayes,Laurence Ma,Thomas Cokelaer,Roland Brosch

Frontiers in immunology 13:1025931 PubMed36569899

2022

B21 DNA vaccine expressing ag85b, rv2029c, and rv1738 confers a robust therapeutic effect against latent infection.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Weng,Jinyi Zhang,Huixia Ma,Jingyu Zhou,Liqiu Jia,Yanmin Wan,Peng Cui,Qiaoling Ruan,Lingyun Shao,Jing Wu,Honghai Wang,Wenhong Zhang,Ying Xu

Frontiers in immunology 12:760468 PubMed34804048

2021

A Dual Marker for Monitoring MDR-TB Treatment: Host-Derived miRNAs and -Derived RNA Sequences in Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Carranza,María Teresa Herrera,Silvia Guzmán-Beltrán,Manuel Guadalupe Salgado-Cantú,Ivan Salido-Guadarrama,Elizabeth Santiago,Leslie Chávez-Galán,Luis Horacio Gutiérrez-González,Yolanda González

EMBO reports 22:e52175 PubMed33938130

2021

Mycobacterium tuberculosis protein kinase G acts as an unusual ubiquitinating enzyme to impair host immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Pupu Ge,Zehui Lei,Zhe Lu,Lihua Qiang,Qiyao Chai,Yong Zhang,Dongdong Zhao,Bingxi Li,Jiaqi Su,Ruchao Peng,Yu Pang,Yi Shi,Yu Zhang,George Fu Gao,Xiao-Bo Qiu,Cui Hua Liu

Frontiers in cellular and infection microbiology 11:634915 PubMed33791241

2021

Aptamer Detection of Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zhou,Huan Xiong,Rong Chen,Lixia Wan,Ying Kong,Jianwei Rao,Yan Xie,Chaolin Huang,Xiao-Lian Zhang

Frontiers in immunology 11:607957 PubMed33391278

2020

Bacille-Calmette-Guérin Infection Aggravates Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Moises A Huaman,Joseph E Qualls,Shinsmon Jose,Stephanie M Schmidt,Anissa Moussa,David G Kuhel,Eddy Konaniah,Ravi K Komaravolu,Carl J Fichtenbaum,George S Deepe,David Y Hui

mSystems 5: PubMed33323416

2020

Role of VapBC12 Toxin-Antitoxin Locus in Cholesterol-Induced Mycobacterial Persistence.

Applications

Unspecified application

Species

Unspecified reactive species

Sakshi Talwar,Manitosh Pandey,Chandresh Sharma,Rintu Kutum,Josephine Lum,Daniel Carbajo,Renu Goel,Michael Poidinger,Debasis Dash,Amit Singhal,Amit Kumar Pandey

Nature communications 11:3062 PubMed32546788

2020

Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Neharika Jain,Haroon Kalam,Lakshyaveer Singh,Vartika Sharma,Saurabh Kedia,Prasenjit Das,Vineet Ahuja,Dhiraj Kumar

Journal of bacteriology 202: PubMed31843799

2020

Polarly Localized EccE Is Required for ESX-1 Function and Stabilization of ESX-1 Membrane Proteins in Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Paloma Soler-Arnedo,Claudia Sala,Ming Zhang,Stewart T Cole,Jérémie Piton
View all publications
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