JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB107585

Anti-MyD88 antibody [4D6]

0

(1 Review)

|

(4 Publications)

Mouse Monoclonal MYD88 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MYD88 aa 50-100.

View Alternative Names

Myeloid differentiation primary response protein MyD88, MYD88

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (AB107585)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (AB107585)

Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 μg/ml; bottom).

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [4D6] (AB107585)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [4D6] (AB107585)

Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.

Western blot - Anti-MyD88 antibody [4D6] (AB107585)
  • WB

Lab

Western blot - Anti-MyD88 antibody [4D6] (AB107585)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MyD88 knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : MOLT4 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 μg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MyD88 antibody [4D6] (ab107585)

Predicted band size: 33 kDa

false

Western blot - Anti-MyD88 antibody [4D6] (AB107585)
  • WB

Unknown

Western blot - Anti-MyD88 antibody [4D6] (AB107585)

All lanes:

Western blot - Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/mL

Lane 1:

Human ovary cell lysate

Lane 2:

Human prostate cell lysate

Lane 3:

Jurkat cell lysate

Lane 4:

Jurkat cell lysate with Immunizing peptide

Secondary

All lanes:

Goat anti-mouse Ig HRP

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D6

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human MYD88 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q99836

Specificity

Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.

style
left-column

Alternative Products

Primary Antibodies

AB133739

Anti-MyD88 antibody [EPR590(N)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [EPR590(N)] (AB133739)

  • 5
  • 1
Primary Antibodies

AB218705

HRP Anti-MyD88 antibody [EPR590(N)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MyD88 antibody [EPR590(N)] (AB218705)

  • 0
  • 0
Primary Antibodies

AB219413

Anti-MyD88 antibody [EPR21824]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (AB219413)

  • 0
  • 0
Primary Antibodies

AB199247

Anti-MyD88 antibody [EPR590(N)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [EPR590(N)] - BSA and Azide free (AB199247)

  • 0
  • 0
Primary Antibodies

AB236766

Anti-MyD88 antibody [EPR21824] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-MyD88 antibody [EPR21824] - BSA and Azide free (AB236766)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB16502

Anti-NF-kB p65 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody (AB16502)

  • 4
  • 40
Primary Antibodies

AB22048

Anti-TLR4 antibody [76B357.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody [76B357.1] (AB22048)

  • 4
  • 29
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5-7 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.5 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.
Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed : 15361868, PubMed : 18292575, PubMed : 33718825, PubMed : 37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 15361868, PubMed : 19506249, PubMed : 24316379, PubMed : 40638072). Increases IL-8 transcription (PubMed : 9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed : 33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
See full target information MYD88
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 20:1579-1589 PubMed40654486

2025

A novel NUTM2A-AS1/miR-769-5p axis regulates LPS-evoked damage in human dental pulp cells via the TLR4/MYD88/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Zeru Wang

Experimental and therapeutic medicine 22:1397 PubMed34650645

2021

Heparin alleviates LPS-induced endothelial injury by regulating the TLR4/MyD88 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxun Liu,Yan Li,Zhaozhao Wu,Kerong Hai,Yun Wang,Xiaohong Zhou,Qingshan Ye

Experimental and therapeutic medicine 20:3733-3739 PubMed32855724

2020

polysaccharides prevent LPS-induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Yin Liu,Li-Li Zhao,Shi-Biao Chen,Ben-Chao Hou,Jian Huang,Xiu Hong,Lian Qing,Yu Fang,Zhe Tao

Experimental and therapeutic medicine 16:2922-2930 PubMed30233666

2018

Exercise intervention alleviates nerve injury by the suppression of inflammatory mediator expression via the TLR4/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Jie Qiong Liu,Feng Tian
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com