Anti-MyD88 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (AB131071)

Immunohistochemical analysis of paraffin-embedded section of human colon cancer tissue labelling MyD88 with ab131071 at 2µg/mL dilution, incubated overnight at 4°C

Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.

Positive staining on human colon cancer tissue.

The tissue section was developed using a HRP Conjugated Rabbit IgG Assay Kit with DAB as the chromogen

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution)

The tissue section was blocked with 10% goat serum.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (AB131071)

Immunohistochemical analysis of paraffin-embedded section of human tonsil tissue labelling MyD88 with ab131071 at 2µg/mL dilution, incubated overnight at 4°C

Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.

Positive staining on human tonsil tissue.

The tissue section was developed using a HRP Conjugated Rabbit IgG Assay Kit with DAB as the chromogen

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution)

The tissue section was blocked with 10% goat serum.

• IP

Supplier Data

Immunoprecipitation - Anti-MyD88 antibody (AB131071)

MYD88 was immunoprecipitated from HepG2 whole cell lysate using ab131071 at 0.5 µg/mL (2 µg in 0.5 mg lysate).

Western blot analysis of MYD88 was performed using ab131071.

Lane 1 : HepG2 whole cell lysate (30 µg).

Lane 2 : Rabbit control IgG instead of ab131071 in HepG2 whole cell lysate.

Lane 3 : ab131071 (2 µg) + HepG2 whole cell lysate (500 µg).

After electrophoresis, proteins were transferred to a membrane. The membrane was then incubated with ab131071 at a dilution of 0.5 µg/mL and probed with a goat anti rabbit IgG HRP secondary antibody. The signal was developed using ECL Plus Western Blotting Substrate.

All lanes:

Immunoprecipitation - Anti-MyD88 antibody (ab131071)

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

Rabbit control IgG

Lane 3:

HepG2 whole cell lysate at 500 µg

Secondary

All lanes:

Goat anti Rabbit IgG HRP

Predicted band size: 33 kDa

Observed band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-MyD88 antibody (AB131071)

All lanes:

Western blot - Anti-MyD88 antibody (ab131071) at 0.5 µg/mL

Lane 1:

Human Jurkat whole cell lysates at 30 µg

Lane 2:

Human HepG2 whole cell lysates at 30 µg

Lane 3:

Human MOLT-4 whole cell lysates at 30 µg

Lane 4:

Human K562 whole cell lysates at 30 µg

Lane 5:

Rat liver tissue lysates at 30 µg

Lane 6:

Mouse liver tissue lysates at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 36 kDa

false