Anti-MyD88 antibody (ab2064) is a rabbit polyclonal antibody detecting MyD88 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.
- KO validated for confirmed specificity
- Over 140 publications
- Trusted since 2000
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- Molecular neurobiology 61:2606-26192023Acupuncture Ameliorates Depression-Like Behaviors Through Modulating the Neuroinflammation Mediated by TLR4 Signaling Pathway in Rats Exposed to Chronic Restraint Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuili Jiang,Xianqi Long,Yu Wang,Xuhui Zhang,Lu Chen,Xinjing Yang,Bingcong Zhao,Ye Zhang,Yemao Chai,Tuya BaoPubMed 37917302
- Folia neuropathologica 61:273-2902023Astragaloside IV inhibits experimental autoimmune encephalomyelitis by modulating the polarization of both microglia/macrophages and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwen Yu,Bingtao Mu,Minfang Guo,Chunyun Liu,Tao Meng,Yuqing Yan,Lijuan Song,Jiezhong Yu,Gajendra Kumar,Cungen MaPubMed 37818688
- Frontiers in pharmacology 14:10584882023Regulation by Nrf2 of IL-1β-induced inflammatory and oxidative response in VSMC and its relationship with TLR4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoe González-Carnicero,Raquel Hernanz,Marta Martínez-Casales,María Teresa Barrús,Ángela Martín,María Jesús AlonsoPubMed 36937865
- Chinese medical journal 136:451-4602023Electroacupuncture-induced activation of GABAergic system alleviates airway inflammation in asthma model by suppressing TLR4/MyD88/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuisong Gong,Xiaowen Liu,Jing ZhaoPubMed 36867547
- Brain, behavior, and immunity 108:204-2202022Adaptor protein MyD88 confers the susceptibility to stress via amplifying immune danger signals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia-Ping Yao,Jian Ye,Ting Feng,Feng-Chao Jiang,Ping Zhou,Fang Wang,Jian-Guo Chen,Peng-Fei WuPubMed 36496170
- Phytotherapy research : PTR 37:731-7422022Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Huang,Tiantian Wu,Youbao Zhong,Jiaqi Huang,Zengping Kang,Bugao Zhou,Haimei Zhao,Duanyong LiuPubMed 36196887
- Evidence-based complementary and alternative medicine : eCAM 2022:61322892022Sishen Pill Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis with Spleen-Kidney Yang Deficiency Syndromes: Role of Gut Microbiota, Fecal Metabolites, Inflammatory Dendritic Cells, and TLR4/NF-B Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Ge,Bu-Gao Zhou,You-Bao Zhong,Su-Qing Liu,Jia-Qi Huang,Wang-Yuan Yuan,Chang-Ying Xie,Duan-Yong Liu,Hai-Yan Wang,Zheng-Yun ZuoPubMed 36310616
- Frontiers in pharmacology 13:9527752022Triptolide in the treatment of systemic lupus erythematosus - regulatory effects on miR-146a in B cell TLR7 signaling pathway in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang,FengQi Zhang,YiNi Gao,MeiJiao Wang,Yan Gao,HaiChang Li,Jing Sun,ChengPing Wen,ZhiJun XiePubMed 36210830
- Evidence-based complementary and alternative medicine : eCAM 2022:16377012022Fuzi-Lizhong Decoction Alleviates Nonalcoholic Fatty Liver Disease by Blocking TLR4/MyD88/TRAF6 Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayao Yang,Ying Zhang,Hongfeng Yi,Yan Liao,Lei Shu,Shu Zhang,Chenyu Li,Liu An,Nianlong Du,Zhaohong Shi,Wei MaPubMed 36065267
- Bioengineered 13:12863-128752022Circular RNA_0001187 participates in the regulation of ulcerative colitis development via upregulating myeloid differentiation factor 88.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Ouyang,Min Wu,Anshan Wu,Heng XiaoPubMed 35609334
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MYD88 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99836
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10-20 µg/mL | Notes - |
Species Mouse | Dilution info 10-20 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825, PubMed:37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:19506249, PubMed:24316379). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
Alternative names
Myeloid differentiation primary response protein MyD88, MYD88
Recommended products
Anti-MyD88 antibody (ab2064) is a rabbit polyclonal antibody detecting MyD88 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.
- KO validated for confirmed specificity
- Over 140 publications
- Trusted since 2000
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MYD88 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99836
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MyD88 antibody (ab2064) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of MyD88?
Anti-MyD88 (ab2064) specifically detects a band for MyD88 (UniProt: Q99836) at a molecular weight of 33kDa.
Trusted by the scientific community
Anti-MyD88 (ab2064) was first used in a scientific publication in 2000 and has been cited over 140 times in peer-reviewed journals.
Reviewed by scientists
Anti-MyD88 (ab2064) has over 10 independent reviews from customers.
Specificity confirmed
The specificity of Anti-MyD88 antibody (ab2064) has been confirmed by Western blot testing in MYD88 Knockout A549 cell line, Human MYD88 knockout A549 cell line ab286715.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.
- Biological function summary
MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.
- Pathways
MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.
- Associated diseases and disorders
MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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10 product images
Western blot - Anti-MyD88 antibody (ab2064)
Western blot: Anti-MYD88 antibody (ab2064) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab2064 was shown to bind specifically to MYD88. A band was observed at 35 kDa in wild-type A549 cell lysates with no signal observed at this size in MYD88 knockout cell line Human MYD88 knockout A549 cell line ab286715 (knockout cell lysate ab290793). To generate this image, wild-type and MYD88 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-MyD88 antibody (ab2064) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: MYD88 knockout A549 cell lysate at 20 µg
Lane 3: HEK-293 cell lysate at 20 µg
Performed under reducing conditions.
Observed band size: 35 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-MyD88 antibody (ab2064)
All lanes: Western blot - Anti-MyD88 antibody (ab2064) at 1 µg/mL
Lanes 1 - 2: Wild type MEFs whole cell lysate
Lanes 3 - 4: MyD88 knockout MEFs whole cell lysate
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 15s
Western blot - Anti-MyD88 antibody (ab2064)
All lanes: Western blot - Anti-MyD88 antibody (ab2064) at 2 µg/mL
Lane 1: A549 cell lysate at 15 µg
Lane 2: HepG2 cell lysate at 15 µg
Lane 3: K562 cell lysate at 15 µg
Lane 4: HT29 cell lysate at 15 µg
Lane 5: HEK293 cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed Jurkat cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (ab2064)
MyD88 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Colon tissue using rabbit Anti-MyD88 antibody
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody (ab2064) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (ab2064)
MyD88 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse heart tissue using rabbit Anti-MyD88 antibody
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody (ab2064) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-MyD88 antibody (ab2064)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-MyD88 antibody (ab2064) at 2 µg/mL
Lane 1: WT HeLa Cells at 10 µg
Lane 2: MyD88 knockout HeLa Cells at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa
Western blot - Anti-MyD88 antibody (ab2064)
1 h incubation at RT in 5% NFDM/TBST.
beta-actin was used as a loading control at 1 µg/mL.
All lanes: Western blot - Anti-MyD88 antibody (ab2064) at 2 µg/mL
Lane 1: HeLa WT cell lysate at 10 µg
Lane 2: MyD88 KO HeLa cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa
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