Goat Polyclonal MYD88 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MYD88 aa 250 to C-terminus.
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- Gut microbes 14:20913692022Intestinal accumulation of microbiota-produced succinate caused by loss of microRNAs leads to diarrhea in weanling piglets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXihong Zhou,Yonghui Liu,Xia Xiong,Jingqing Chen,Wenjie Tang,Liuqin He,Zhigang Zhang,Yulong Yin,Fengna LiPubMed 35758253
- Kidney diseases (Basel, Switzerland) 8:231-2452022Long Noncoding RNA MEG3-205/Let-7a/MyD88 Axis Promotes Renal Inflammation and Fibrosis in Diabetic Nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQimei Luo,Xi Xia,Qingqing Luo,Yue Qiu,Lan Dong,Chen Zhao,Fenfen Peng,Jing Yu,Fengxian Huang,Feng HePubMed 35702702
- International journal of molecular sciences 23:2022Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Yu Chang,Hsuan-Miao Liu,Yann-Lii Leu,Chung-Hua Hsu,Tzung-Yan LeePubMed 35269806
- Frontiers in pharmacology 12:6932982021Glycoproteins From var. Regulate Macrophage Polarization and Alleviate Lipopolysaccharide-Induced Acute Lung Injury in Mice TLR4/NF-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn-Qi Ren,Hui-Jun Wang,Hai-Yan Zhu,Guan Ye,Kun Li,Dao-Feng Chen,Tao Zeng,Hong LiPubMed 34366849
- Stem cells international 2020:21345652020Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Yang et. al.PubMed 32300366
- European journal of oral sciences 118:333-412010MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDawen Liu,Shaomian Yao,Gary E WisePubMed 20662905
- Oncogene 28:4353-632009TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer.Applications:IHC-PReactive species:HumanM Szajnik,M J Szczepanski,M Czystowska,E Elishaev,M Mandapathil,E Nowak-Markwitz,M Spaczynski,T L WhitesidePubMed 19826413
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human MYD88 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99836
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Reactivity data
Select an application| ICC | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Dog | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-0.3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4-6 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Dog | Dilution info - | Notes - |
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Target data
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:24316379, PubMed:19506249). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
Alternative names
Myeloid differentiation primary response protein MyD88, MYD88
Recommended products
Goat Polyclonal MYD88 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MYD88 aa 250 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human MYD88 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99836
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Flow Cytometry - Anti-MyD88 antibody (ab28763)
Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton using ab28763 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 1ug/ml. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (ab28763)
ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab28763)
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab28763)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Western blot - Anti-MyD88 antibody (ab28763)
Lysates in RIPA buffer. Detected by chemiluminescence.
All lanes: Western blot - Anti-MyD88 antibody (ab28763) at 0.5 µg/mL
All lanes: Human Spleen lysate at 35 µg
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