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AB28763

Anti-MyD88 antibody

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(11 Publications)

Goat Polyclonal MYD88 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human MYD88 aa 250-300.

View Alternative Names

Myeloid differentiation primary response protein MyD88, MYD88

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (AB28763)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (AB28763)

ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Flow Cytometry - Anti-MyD88 antibody (AB28763)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-MyD88 antibody (AB28763)

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton using ab28763 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 1ug/ml. IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB28763)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB28763)

Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB28763)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB28763)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western blot - Anti-MyD88 antibody (AB28763)
  • WB

Supplier Data

Western blot - Anti-MyD88 antibody (AB28763)

Lysates in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-MyD88 antibody (ab28763) at 0.5 µg/mL

All lanes:

Human Spleen lysate at 35 µg

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MYD88 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q99836

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.
Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed : 15361868, PubMed : 18292575, PubMed : 33718825, PubMed : 37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 15361868, PubMed : 19506249, PubMed : 24316379, PubMed : 40638072). Increases IL-8 transcription (PubMed : 9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed : 33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
See full target information MYD88
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:133-148 PubMed39735680

2024

CAF-derived exosome-miR-3124-5p promotes malignant biological processes in NSCLC via the TOLLIP/TLR4-MyD88-NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Sun,Qinghua Song,Hua Liu

Gut microbes 16:2313769 PubMed38353638

2024

Gut microbiota regulates host melatonin production through epithelial cell MyD88.

Applications

Unspecified application

Species

Unspecified reactive species

Bingnan Liu,Lijuan Fan,Youxia Wang,Hao Wang,Yuqi Yan,Shuai Chen,Ifen Hung,Chunxue Liu,Hong Wei,Liangpeng Ge,Wenkai Ren

Gut microbes 14:2091369 PubMed35758253

2022

Intestinal accumulation of microbiota-produced succinate caused by loss of microRNAs leads to diarrhea in weanling piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Xihong Zhou,Yonghui Liu,Xia Xiong,Jingqing Chen,Wenjie Tang,Liuqin He,Zhigang Zhang,Yulong Yin,Fengna Li

iScience 25:104561 PubMed35769880

2022

The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Chen,Runliu Wu,Jiao Liu,Rui Kang,Jinbao Li,Daolin Tang

Kidney diseases (Basel, Switzerland) 8:231-245 PubMed35702702

2022

Long Noncoding RNA MEG3-205/Let-7a/MyD88 Axis Promotes Renal Inflammation and Fibrosis in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qimei Luo,Xi Xia,Qingqing Luo,Yue Qiu,Lan Dong,Chen Zhao,Fenfen Peng,Jing Yu,Fengxian Huang,Feng He

Frontiers in pharmacology 13:838261 PubMed35370734

2022

Mechanistic Studies of Gypenosides in Microglial State Transition and its Implications in Depression-Like Behaviors: Role of TLR4/MyD88/NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hua Cao,Yuan-Yuan Zhao,Ming Bai,David Geliebter,Jan Geliebter,Raj Tiwari,Hong-Juan He,Zhen-Zhen Wang,Xing-Yuan Jia,Jin Li,Xiu-Min Li,Ming-San Miao

International journal of molecular sciences 23: PubMed35269806

2022

Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Yu Chang,Hsuan-Miao Liu,Yann-Lii Leu,Chung-Hua Hsu,Tzung-Yan Lee

Frontiers in pharmacology 12:693298 PubMed34366849

2021

Glycoproteins From var. Regulate Macrophage Polarization and Alleviate Lipopolysaccharide-Induced Acute Lung Injury in Mice TLR4/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

An-Qi Ren,Hui-Jun Wang,Hai-Yan Zhu,Guan Ye,Kun Li,Dao-Feng Chen,Tao Zeng,Hong Li

Stem cells international 2020:2134565 PubMed32300366

2020

Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Yang,Shengnan Sun,Qi Zhang,Jia Guo,Tengfei Wu,Ying Liu,Min Yang,Yan Zhang,Yinghua Peng

European journal of oral sciences 118:333-41 PubMed20662905

2010

MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption.

Applications

Unspecified application

Species

Unspecified reactive species

Dawen Liu,Shaomian Yao,Gary E Wise
View all publications
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