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Anti-MyD88 antibody [EPR21824] (ab219413) is a rabbit monoclonal antibody that is used to detect MyD88 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with MyD88 knockout cell line validation

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Images

Western blot - Anti-MyD88 antibody [EPR21824] (AB219413), expandable thumbnail
  • Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (AB219413), expandable thumbnail
  • Western blot - Anti-MyD88 antibody [EPR21824] (AB219413), expandable thumbnail
  • Western blot - Anti-MyD88 antibody [EPR21824] (AB219413), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

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Target data

Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825, PubMed:37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:19506249, PubMed:24316379). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).

Alternative names

Myeloid differentiation primary response protein MyD88, MYD88

Recommended products

Anti-MyD88 antibody [EPR21824] (ab219413) is a rabbit monoclonal antibody that is used to detect MyD88 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with MyD88 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21824
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-MyD88 antibody [EPR21824] (ab219413) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

What is the molecular weight of MyD88?


Anti-MyD88 [EPR21824] (ab219413) specifically detects a band for MyD88 (UniProt: P22366) at a molecular weight of 33kDa.

Trusted by the scientific community


Anti-MyD88 [EPR21824] (ab219413) was first used in a scientific publication in 2018 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-MyD88 antibody [EPR21824] (ab219413) has been confirmed by Western blot testing in MYD88 Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR21824] also available for your convenience:
ab219413, Carrier free - Anti-MyD88 antibody [EPR21824] - BSA and Azide free ab236766



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.

Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

Associated diseases and disorders

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413), expandable thumbnail

    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique

    All lanes: Western blot - Anti-MyD88 antibody [EPR21824] (ab219413) at 1/100000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: MyD88 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413), expandable thumbnail

    Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)

    MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.


    Lane 1: RAW 264.7 whole cell lysate 10 μg (Input).
    Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)

    Predicted band size: 33 kDa

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413), expandable thumbnail

    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1: Rat liver lysate at 20 µg

    Lane 2: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 3: A20 (Mouse reticulum sarcoma B lymphocyte) cell lysate at 20 µg

    Lane 4: Mouse liver lysate at 20 µg

    Lane 5: Mouse lung lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Exposure time: 3min

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413), expandable thumbnail

    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)

    False colour image of Western blot: Anti-MyD88 antibody [EPR21824] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab219413 was shown to bind specifically to MyD88. A band was observed at 35 kDa in wild-type A549 cell lysates with no signal observed at this size in MYD88 knockout cell line Human MYD88 knockout A549 cell line ab286715 (knockout cell lysate ab290793). To generate this image, wild-type and MYD88 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: MYD88 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human MYD88 knockout A549 cell line (Human MYD88 knockout A549 cell line ab286715)

    Lane 3: HEK-293 cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD

    Lanes 1 - 3: Goat anti-Rabbit IgG H&L 800CW

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 35 kDa

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Product protocols

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