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AB119048

Anti-MyD88 antibody [OTI1B4]

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(2 Publications)

Mouse Monoclonal MYD88 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYD88.

View Alternative Names

Myeloid differentiation primary response protein MyD88, MYD88

6 Images
Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)

ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)

ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)

ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).

Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048)
  • WB

Lab

Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MyD88 knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : Molt-4 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.

ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab119048 and ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 andGoat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)

Predicted band size: 33 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (AB119048)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (AB119048)

ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.

Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048)
  • WB

Unknown

Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048)

All lanes:

Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048) at 1/2000 dilution

Lane 1:

HEK293T lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA at 5 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1B4

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MYD88.

Q99836

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.
Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed : 15361868, PubMed : 18292575, PubMed : 33718825, PubMed : 37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 15361868, PubMed : 19506249, PubMed : 24316379, PubMed : 40638072). Increases IL-8 transcription (PubMed : 9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed : 33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
See full target information MYD88
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMJ open diabetes research & care 11: PubMed37085277

2023

Ghrelin attenuates inflammation in diabetic lung disease by TLR4 pathway in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Liu,Dong-Guang Wei,Rong-Shan Li

Cellular physiology and biochemistry : internation 42:91-102 PubMed28494464

2017

Immunoregulatory Role of MicroRNA-21 in Macrophages in Response to Bacillus Calmette-Guerin Infection Involves Modulation of the TLR4/MyD88 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xue,Yi Qiu,Hong-Li Yang
View all publications
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Product promise

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