Mouse Monoclonal MYD88 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYD88.
Images
![Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--western-blot-img99292.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048)")
![Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--western-blot-img141999.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MyD88 antibody [OTI1B4] (AB119048)")
![Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (AB119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--immunocytochemistry-immunofluorescence-img3025.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (AB119048)")
![Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--flow-cytometry-intracellular-img58571.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)")
![Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--flow-cytometry-intracellular-img52262.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (AB119048)")
Publications
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- BMJ open diabetes research & care 11:2023Ghrelin attenuates inflammation in diabetic lung disease by TLR4 pathway in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Yan Liu,Dong-Guang Wei,Rong-Shan LiPubMed 37085277
- Cellular physiology and biochemistry : internation 42:91-1022017Immunoregulatory Role of MicroRNA-21 in Macrophages in Response to Bacillus Calmette-Guerin Infection Involves Modulation of the TLR4/MyD88 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Xue et. al.PubMed 28494464
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MYD88. Database link Q99836
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Transfected cell line - Human | Not recommended | Tested | Tested |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/100 | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/100 | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:24316379, PubMed:19506249). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
Alternative names
Myeloid differentiation primary response protein MyD88, MYD88
Recommended products
Mouse Monoclonal MYD88 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYD88.
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MYD88. Database link Q99836
- Clone number
OTI1B4
- Purification technique
Affinity purification
- Concentration
- Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
![Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--western-blot-img99292.jpg "Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)")
Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 μg)
Lane 3: Jurkat cell lysate (20 μg)
Lane 4: Molt-4 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 52 kDa.ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab119048 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 andGoat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)
Predicted band size: 33 kDa
![Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--western-blot-img141999.jpg "Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)")
Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048)
All lanes: Western blot - Anti-MyD88 antibody [OTI1B4] (ab119048) at 1/2000 dilution
Lane 1: HEK293T lysate transfected with pCMV6-ENTRY control cDNA at 5 µg
Lane 2: HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA at 5 µg
Predicted band size: 33 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--immunocytochemistry-immunofluorescence-img3025.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (ab119048)")
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [OTI1B4] (ab119048)
ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.
![Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--flow-cytometry-intracellular-img58571.jpg "Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)")
Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)
ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).
![Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--flow-cytometry-intracellular-img52262.jpg "Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)")
Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)
ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
![Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--flow-cytometry-intracellular-img80999.jpg "Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)")
Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)
ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
![Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048), expandable thumbnail](https://content.abcam.com/products/images/myd88-antibody-oti1b4-ab119048--flow-cytometry-intracellular-img80999.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MyD88 antibody [OTI1B4] (ab119048)")
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