Rat Recombinant Monoclonal Myelin Basic Protein antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182225.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)")
![Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--western-blot-img185952.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176160.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)")
![Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--western-blot-img166978.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)")
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--immunocytochemistry-immunofluorescence-img428726.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Tested | Not recommended | Tested |
| Rat | Tested | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Alternative names
Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Recommended products
Rat Recombinant Monoclonal Myelin Basic Protein antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 12
- Purification technique
- Ion exchange chromatography
- Epitope
- Amino acids 82-87 (DENPVV).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab277483 is the carrier-free version of Anti-Myelin Basic Protein antibody [12] ab7349.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.
- Biological function summary
MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.
- Pathways
MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.
- Associated diseases and disorders
MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182225.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with Anti-Myelin Basic Protein antibody [12] ab7349 at 1/4000 dilution (0.259 μg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with Anti-Myelin Basic Protein antibody [12] ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--western-blot-img185952.jpg "Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)")
Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
This data was developed using Anti-Myelin Basic Protein antibody [12] ab7349, the same antibody clone in a different buffer formulation.
The molecular weight observed was in consistency with the literature (PMID: 9299539).
Blocking buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483) at 1/5000 dilution
All lanes: Human cerebellum tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 18.5 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176160.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with Anti-Myelin Basic Protein antibody [12] ab7349 at 1/4000 dilution (0.259 μg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with Anti-Myelin Basic Protein antibody [12] ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with DAPI.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
![Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--western-blot-img166978.jpg "Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)")
Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
This data was developed using the Anti-Myelin Basic Protein antibody [12] ab7349, the same antibody clone in a different buffer formulation.
The molecular weight observed was in consistency with the literature (PMID: 9299539).
Blocking buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lane 2: 1 second.
All lanes: Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483) at 1/5000 dilution
Lane 1: Mouse brain tissue lysate at 10 µg
Lane 2: Rat brain tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 14-21.5 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483--immunocytochemistry-immunofluorescence-img428726.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)")
Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with Anti-Myelin Basic Protein antibody [12] ab7349 at a 1/200 dilution, followed by Goat anti-Rat (Alexa Fluor® 488) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Counterstained with Anti-MAP2 antibody (Anti-MAP2 antibody [EPR19691] ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at a 1/1000 dilution (shown in magenta).
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The negative controls are as follows:
-ve control 1: Myelin Basic Protein (Anti-Myelin Basic Protein antibody [12] ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at a 1/1000 dilution.
-ve control 2: Anti-MAP2 (Anti-MAP2 antibody [EPR19691] ab183830) at a 1/1000 dilution, followed by Goat anti-Rat (Alexa Fluor® 488) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at a 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Myelin Basic Protein antibody [12] ab7349).
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