Anti-Myelin Basic Protein antibody [12] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)

Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 μg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)

Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 μg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with DAPI.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with ab7349 at a 1/200 dilution, followed by Goat anti-Rat (Alexa Fluor^® 488) (ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Counterstained with Anti-MAP2 antibody (ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor^® 594) (ab150080) at a 1/1000 dilution (shown in magenta).

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The negative controls are as follows :

-ve control 1 : Myelin Basic Protein (ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor^® 594) (ab150080) at a 1/1000 dilution.

-ve control 2 : Anti-MAP2 (ab183830) at a 1/1000 dilution, followed by Goat anti-Rat (Alexa Fluor^® 488) (ab150157) at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7349).

• WB

Supplier Data

Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)

This data was developed using ab7349, the same antibody clone in a different buffer formulation.

The molecular weight observed was in consistency with the literature (PMID : 9299539).

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483) at 1/5000 dilution

All lanes:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 18.5 kDa

true

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (AB277483)

This data was developed using the ab7349, the same antibody clone in a different buffer formulation.

The molecular weight observed was in consistency with the literature (PMID : 9299539).

Blocking buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 seconds; Lane 2 : 1 second.

All lanes:

Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 14-21.5 kDa

false