AB216590

Anti-Myelin Basic Protein antibody

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(5 Publications)

Rabbit Polyclonal Myelin Basic Protein antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Guinea pig MBP aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Shi, Mbp, Myelin basic protein, MBP, Myelin A1 protein

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB216590)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB216590)

Immunofluorescence analysis of formalin-fixed rat brain tissue, labeling Myelin Basic Protein using ab216590 at a 1/200 dilution; followed by secondary antibody PE-conjugated goat anti-rabbit lgG at a 1/200 dilution. Cell nuclei were stained with 5 μL/mL DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB216590)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB216590)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling Myelin Basic Protein using ab216590 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB216590)

Supplier Data

Western blot - Anti-Myelin Basic Protein antibody (AB216590)

All lanes:

Western blot - Anti-Myelin Basic Protein antibody (ab216590) at 1/300 dilution

All lanes:

mouse brain lysate

Predicted band size: 33 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Guinea pig MBP aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25188

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.

Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.

See full target information Mbp

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:343 PubMed41022714

2025

Deep brain stimulation of medial forebrain bundle modulates noradrenergic activity and feedforward inhibition in rodent model of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Duan,Yixin Tong,Volker A Coenen,Máté D Döbrössy

Journal of nanobiotechnology 22:106 PubMed38468300

2024

Nanoscale myelinogenesis image in developing brain via super-resolution nanoscopy by near-infrared emissive curcumin-BODIPY derivatives.

Applications

Unspecified application

Species

Unspecified reactive species

Junyang Chen,Yifan Yu,Siyou Wang,Yu Shen,Yupeng Tian,Loris Rizzello,Kui Luo,Xiaohe Tian,Tinghua Wang,Liulin Xiong

International journal of molecular sciences 24: PubMed38069032

2023

Attenuation of Vanadium-Induced Neurotoxicity in Rat Hippocampal Slices (In Vitro) and Mice (In Vivo) by ZA-II-05, a Novel NMDA-Receptor Antagonist.

Applications

Unspecified application

Species

Unspecified reactive species

Amany Digal Ladagu,Funmilayo Eniola Olopade,Paul Chazot,Ademola A Oyagbemi,Samuel Ohiomokhare,Oluwabusayo Racheal Folarin,Taidinda Tashara Gilbert,Madison Fuller,Toan Luong,Adeboye Adejare,James O Olopade

Cell death & disease 14:736 PubMed37952053

2023

FKBP5 activates mitophagy by ablating PPAR-γ to shape a benign remyelination environment.

Applications

Unspecified application

Species

Unspecified reactive species

Xingzong Sun,Menghan Qian,Hongliang Li,Lei Wang,Yunjie Zhao,Min Yin,Lili Dai,Hongkun Bao

eLife 7: PubMed29972353

2018

Loss of from neurons regulating hypoxic and hypercapnic chemoresponses causes neonatal respiratory failure in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Meike E van der Heijden,Huda Y Zoghbi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Myelin Basic Protein antibody

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB216590)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB216590)

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB216590)

Western blot - Anti-Myelin Basic Protein antibody (AB216590)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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