AB28541

Anti-Myelin Basic Protein antibody

Lab Essentials
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(3 Publications)

Rabbit Polyclonal Myelin Basic Protein antibody. Suitable for sELISA and reacts with Pig samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow MBP.

View Alternative Names

Myelin basic protein, MBP

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Sandwich ELISA - Anti-Myelin Basic Protein antibody (AB28541)

sELISA

Unknown

Sandwich ELISA - Anti-Myelin Basic Protein antibody (AB28541)

Immobilized Anti-MBP (ab28541) : range from 0.125 to 2 μg/ml, 100 μl/well
Fixed Captured Antigen : Swine MBP, 500 ng/mL
Detection : anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt

sELISA

Unknown

Sandwich ELISA - Anti-Myelin Basic Protein antibody (AB28541)

Fixed Immobilized Anti-MBP (ab28541) : 2 ug/mL, 100 ul/well
Captured Antigen : Swine MBP , range from 1 to 90 ng/mL
Detection : anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Pig

Applications

sELISA

applications

Immunogen

Native Full Length Protein corresponding to Cow MBP.

P02687

Specificity

This antibody is specific to myelin basic proteins. It may cross react with other isoforms of myelin basic proteins.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification notes

The antibody was affinity purified on agarose with an immuno dominant peptide (PRTPPPSQGKGRGL), which is a universal peptide sequence for all isoforms of mammalian myelin basic proteins.

Storage buffer

pH: 6 - 8 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.01% Ammonium Acetate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.

Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity).

See full target information MBP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:19529 PubMed37945646

2023

Artificial axons as a biomimetic 3D myelination platform for the discovery and validation of promyelinating compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Jagielska,Kristin Radzwill,Daniela Espinosa-Hoyos,Mingyu Yang,Kavin Kowsari,Jonathan E Farley,Stefanie Giera,Ann Byrne,Guoqing Sheng,Nicholas X Fang,James C Dodge,Carlos E Pedraza,Krystyn J Van Vliet

Reproductive sciences (Thousand Oaks, Calif.) 29:1296-1304 PubMed34462873

2021

Identification of Adrenomedullin-Induced S-Nitrosylated Proteins in JEG-3 Placental Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingting Li,Liuying Zhong,Cheuk-Lun Lee,Philip C N Chiu,Min Chen

Science advances 6: PubMed33328221

2020

Spatiotemporal cellular movement and fate decisions during first pharyngeal arch morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yuan,Yong-Hwee Eddie Loh,Xia Han,Jifan Feng,Thach-Vu Ho,Jinzhi He,Junjun Jing,Kimberly Groff,Alan Wu,Yang Chai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myelin Basic Protein antibody

Sandwich ELISA - Anti-Myelin Basic Protein antibody (AB28541)

Sandwich ELISA - Anti-Myelin Basic Protein antibody (AB28541)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise