Anti-Myelin Basic Protein antibody

• WB

Supplier Data

Western blot - Anti-Myelin Basic Protein antibody (AB65988)

Western blot analysis of Myelin Basic Protein using Anti-Myelin Basic Protein antibody.

Blocked with 5% non-fat milk/TBS.

The signal was developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system.

A specific band was detected for MBP at approximately 15-22 kDa, MBP can appear as multiple bands around 18-22 kDa due to alternative splicing.. The expected band size for MBP is at 33 kDa.

All lanes:

Western blot - Anti-Myelin Basic Protein antibody (ab65988) at 0.5 µg/mL

Lane 1:

Rat brain tissue lysates, at 30 µg

Lane 2:

Mouse brain tissue lysates at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody

Observed band size: 15-22 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of human brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB65988)

Immunofluorescence analysis of Myelin Basic Protein using Anti-Myelin Basic Protein antibody. Myelin Basic Protein was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL ab65988 overnight at 4°C. HRP Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 570 reagent at 1 : 200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 570 and DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB65988)

Immunofluorescence analysis of Myelin Basic Protein using Anti-Myelin Basic Protein antibody. Myelin Basic Protein was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL ab65988 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB65988)

Immunofluorescence analysis of Myelin Basic Protein using Anti-Myelin Basic Protein antibody. Myelin Basic Protein was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL ab65988 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody (AB65988)

Immunofluorescence analysis of Myelin Basic Protein using Anti-Myelin Basic Protein antibody. Myelin Basic Protein was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL ab65988 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of human brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of pig brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of mouse brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of pig brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of mouse brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (AB65988)

Immunohistochemical analysis of paraffin-embedded section of rat brain tissue stained for Myelin Basic Protein with ab65988 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).