JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB324098

Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Myelin Basic Protein antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.

View Alternative Names

Shi, Myelin basic protein, MBP, Myelin A1 protein

7 Images
Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Sandwich ELISA with the capture antibody ab324098 dilution at 2 μg/mL and detector antibody ab324376 dilution at 0.5 μg/mL.

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Interpolated concentration of native Myelin Basic Protein isoform 5 was measured at different sample concentrations. Undiluted sample is as follows : WM-266-4 cell extract (250 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Sandwich ELISA with the capture antibody ab324097 dilution at 2 ug/mL and detector antibody ab324098 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Interpolated concentration of native Myelin Basic Protein was measured at different sample concentrations. Undiluted sample is as follows : mouse brain tissue extract (0.63 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Interpolated concentration of native Myelin Basic Protein was measured at different sample concentrations. Undiluted sample is as follows : rat brain tissue extract (0.16 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Interpolated concentration of native Myelin Basic Protein isoform 5 was measured at different sample concentrations. Undiluted samples are as follows : mouse brain supernatant 2% and rat brain supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myelin Basic Protein antibody [EPR29339-55] - BSA and Azide free (Capture/Detector) (AB324098)

Interpolated concentration of native Myelin Basic Protein was measured at different sample concentrations. Undiluted samples are as follows : mouse brain supernatant 0.5% and rat brain supernatant 0.063%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29339-55

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab324098 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse, Rat Myelin Basic Protein. The recommended pair for sandwich ELISA is:

Capture: ab324097, Mouse, Rat Myelin Basic Protein Capture Antibody (unconjugated)

Detector: ab324098, Human, Mouse, Rat Myelin Basic Protein Detector Antibody (unconjugated)

The reference range value is 15.6-1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.
See full target information Mbp
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB150675

Luxol Fast Blue Stain Kit (Myelin Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

  • 3
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com