Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)

Rabbit Recombinant Monoclonal Myelin Basic Protein antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Mouse Mbp conjugated to Keyhole Limpet Haemocyanin.

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Images

Immunohistochemistry (Frozen sections) - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (AB313827), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Mouse Mbp conjugated to Keyhole Limpet Haemocyanin. Database link P04370

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	IHC-Fr
Human	Tested	Expected	Expected	Expected
Mouse	Tested	Tested	Expected	Tested
Rat	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000.00000 - 1/500000.00000	Notes -
Species Mouse	Dilution info 1/5000.00000 - 1/500000.00000	Notes -
Species Rat	Dilution info 1/5000.00000 - 1/500000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/200	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/250.00000 - 1/500.00000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information Mbp

Function

The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.

Additional Targets

Mbp, MBP

Alternative names

Shi, Mbp, Myelin basic protein, MBP, Myelin A1 protein

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Mouse Mbp conjugated to Keyhole Limpet Haemocyanin. Database link P04370
Carrier free: Yes
Clone number: HL1033
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.

Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

Associated diseases and disorders

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunohistochemistry (Frozen sections) - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunohistochemical analysis of Frozen-sectioned mouse cerebellum staining Myelin basic protein with ab313827 at a dilution of 1/100 (Green).
Immunohistochemistry (Frozen sections) - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunohistochemical analysis of Frozen-sectioned mouse cerebellum staining Myelin basic protein with ab313827 at a dilution of 1/100 (Green). The nuclear counterstaining was Hoechst 33342 (Blue).
Immunohistochemistry (Frozen sections) - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunohistochemical analysis of Frozen-sectioned mouse hippocampus staining Myelin basic protein with ab313827 at a dilution of 1/100 (Green). The nuclear counterstaining was Hoechst 33342 (Blue).
Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Samples were seperated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827) at 1/500000 dilution
Lane 1: Mouse brain tissue at 50 µg
Lane 2: Rat brain tissue at 50 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 27 kDa
Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Samples were seperated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827) at 1/10000 dilution
All lanes: Human brain tissue extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 27 kDa
Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
All lanes: Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827) at 1/500000 dilution
Lane 1: Mouse brain tissue extract at 50 µg
Lane 2: Rat brain tissue extract at 50 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
All lanes: Western blot - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827) at 1/10000 dilution
All lanes: Human brain tissue extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeleing Myelin basic protein using ab313827 at a 1/200 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeleing Myelin basic protein using ab313827 at a 1/200 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunofluorescent analysis of DIV9 rat cortical neurons and glia labeling Myelin basic Protein using ab313827 at a 1/250 dilution (green). Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1/500. Blue: Fluoroshield with DAPI.

Cells were fixed with 4% paraformaldehyde at RT for 15 min.
Immunocytochemistry/ Immunofluorescence - Anti-Myelin basic protein antibody [HL1033] - BSA and Azide free (ab313827)
Immunofluorescent analysis of DIV10 rat cortical neuron-no AraC cells (at oligodendrocyte) labeling Myelin basic Protein using ab313827 at a 1/250 dilution (green). Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1/500.

Cells were fixed with 4% paraformaldehyde at RT for 15 min.