Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Myelin Basic Protein in Flow Cytometry. Suitable for Human, Rat.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 100 publications
- Trusted since 2008
Images
![Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (AB62631), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-mbp101-bsa-and-azide-free-ab62631--immunocytochemistry-img41290.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (AB62631)")
![Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (AB62631), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-mbp101-bsa-and-azide-free-ab62631--flow-cytometry-img112907.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (AB62631)")
Publications
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- Communications medicine 3:1702023AAV-mediated editing of PMP22 rescues Charcot-Marie-Tooth disease type 1A features in patient-derived iPS Schwann cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Yoshioka,Juliana Bosso Taniguchi,Hidenori Homma,Takuya Tamura,Kyota Fujita,Maiko Inotsume,Kazuhiko Tagawa,Kazuharu Misawa,Naomichi Matsumoto,Masanori Nakagawa,Haruhisa Inoue,Hikari Tanaka,Hitoshi OkazawaPubMed 38017287
- Neural regeneration research 18:1976-19822023C-X-C chemokine receptor type 7 antibody enhances neural plasticity after ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Qian Zhang,Xiao-Yin Wang,Bing-Chao Dong,Mei-Xuan Li,Yu Wang,Ting Xiao,Shan-Shan ZhaoPubMed 36926722
- Scientific reports 13:22332023Engineering of an electrically charged hydrogel implanted into a traumatic brain injury model for stepwise neuronal tissue reconstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Tanikawa,Yuki Ebisu,Tomáš Sedlačík,Shingo Semba,Takayuki Nonoyama,Takayuki Kurokawa,Akira Hirota,Taiga Takahashi,Kazushi Yamaguchi,Masamichi Imajo,Hinako Kato,Takuya Nishimura,Zen-Ichi Tanei,Masumi Tsuda,Tomomi Nemoto,Jian Ping Gong,Shinya TanakaPubMed 36788295
- Viruses 15:2023Early Permissiveness of Central Nervous System Cells to Measles Virus Infection Is Determined by Hyperfusogenicity and Interferon Pressure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Ferren,Alexandre Lalande,Mathieu Iampietro,Lola Canus,Didier Decimo,Denis Gerlier,Matteo Porotto,Cyrille MathieuPubMed 36680268
- Frontiers in neuroscience 16:9260232022High phenylalanine concentrations induce demyelination and microglial activation in mouse cerebellar organotypic slices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOrli Thau-Zuchman,Patrick N Pallier,Paul J M Savelkoul,Almar A M Kuipers,J Martin Verkuyl,Adina T Michael-TitusPubMed 36248632
- Cells 11:2022Dexras1 Induces Dysdifferentiation of Oligodendrocytes and Myelin Injury by Inhibiting the cAMP-CREB Pathway after Subarachnoid Hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanjun Xin,Jie Chen,Hongxia Zhang,Robert P Ostrowski,Yidan Liang,Jun Zhao,Xiang Xiang,Fuming Liang,Wenqiao Fu,Hao Huang,Xintong Wu,Jun Su,Jiewen Deng,Zhaohui HePubMed 36230939
- Cell death & disease 13:7592022Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingting Jin,Jingjing Zhang,Xin Hua,Xingxing Xu,Jia Li,Jiaojiao Wang,Mianxian Wang,Huitao Liu,Haoyu Qiu,Man Chen,Xu Zhang,Ying Wang,Zhihui HuangPubMed 36055989
- CNS neuroscience & therapeutics 28:2183-21942022Lomitapide ameliorates middle cerebral artery occlusion-induced cerebral ischemia/reperfusion injury by promoting neuronal autophagy and inhibiting microglial migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangmin Zheng,Yue Hu,Ziping Han,Feng Yan,Sijia Zhang,Zhenhong Yang,Fangfang Zhao,Lingzhi Li,Junfen Fan,Rongliang Wang,Yumin LuoPubMed 36052650
- The Korean journal of pain 35:160-1722022Insulin enhances neurite extension and myelination of diabetic neuropathy neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVuong M Pham,Nitish ThakorPubMed 35354679
- PLoS biology 20:e30015902022Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica L Gorenberg,Sofia Massaro Tieze,Betül Yücel,Helen R Zhao,Vicky Chou,Gregory S Wirak,Susumu Tomita,TuKiet T Lam,Sreeganga S ChandraPubMed 35358180
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human MBP. Database link P02686
Reactivity data
Select an application| ICC | Flow Cyt | |
|---|---|---|
| Human | Expected | Tested |
| Rat | Tested | Expected |
| Primates | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Alternative names
Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Recommended products
Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Myelin Basic Protein in Flow Cytometry. Suitable for Human, Rat.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 100 publications
- Trusted since 2008
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human MBP. Database link P02686
- Carrier free
- Yes
- Clone number
- MBP101
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified antibody
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human, Rat samples.
Trusted by the scientific community
Anti-Myelin Basic Protein [MBP101] - BSA and Azide free (ab62631) was first used in a scientific publication in 2008 and has been cited over 100 times in peer-reviewed journals.
Reviewed by scientists
Anti-Myelin Basic Protein [MBP101] - BSA and Azide free (ab62631) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.
- Biological function summary
MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.
- Pathways
MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.
- Associated diseases and disorders
MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-mbp101-bsa-and-azide-free-ab62631--immunocytochemistry-img41290.jpg "Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)")
Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)
ab62631 staining Myelin Basic Protein in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab62631 at 1μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
![Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631), expandable thumbnail](https://content.abcam.com/products/images/myelin-basic-protein-antibody-mbp101-bsa-and-azide-free-ab62631--flow-cytometry-img112907.jpg "Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)")
Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)
Overlay histogram showing SH-SY5Y cells stained with ab62631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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