AB11159

Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker

BOND RX™ Validated
Recombinant
Lab Essentials
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(8 Publications)

Mouse Recombinant Monoclonal Myelin Basic Protein antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myelin Basic Protein with ab11159 at 1 : 1000 (1.125 μg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control : No staining on human spleen (PMID : 9518636). The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at /10 (112.5 ug/ml) dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor 488) at 1000 dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor 488)at 1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Supplier Data

Western blot - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (ab11159) at 1/1000 dilution

All lanes:

human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/50000 dilution

Predicted band size: 33 kDa

false

Exposure time: 26s

Carrier free

Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MBP2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/10", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/10", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)." } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.

Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

See full target information MBP

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroengineering and rehabilitation 22:170 PubMed40691625

2025

Transcutaneous auricular vagus nerve stimulation enhances structural and functional remodeling in sensorimotor networks following intracerebral hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Zengyu Zhang,Lichao Wei,Dewen Ru,Meihua Wang,Pengfei Fu,Weijian Yang,Gang Wu,Lei Yang,Caihua Xi,Xiangru Ye,Yuwen Zhang,He Wang,Jin Hu,Qiang Yuan

IBRO neuroscience reports 18:627-637 PubMed40584426

2025

Neural stem cell changes and spatial distribution of AQP4 expression in a fetal goat model of obstructive hydrocephalus.

Applications

Unspecified application

Species

Unspecified reactive species

Xinting Ji,Qin Zhang,Yuantao Yang,Yan Li,Lihong Li,Shibin Lin,Qi Dong,Zhenling Wan,Julan Wu,Jiadong Bao,Xiaoman Su,Pengzhi Chen,Chenyuan Liu,Weian Song,Zechen Wang,Kang Feng,Xiaoying Li,Shaojiang Zheng,Shuo Gu

Cell biochemistry and biophysics 83:1771-1783 PubMed39681812

2024

METTL3 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells Under the Inflammatory Microenvironment Through the miR-141-3p/ZEB1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weijia Li,Adili Alimujiang

Neurochemical research 50:41 PubMed39613948

2024

Induction of Neural Differentiation and Protection by a Novel Slow-Release Nanoparticle Estrogen Construct in a Rat Model of Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Azizul Haque,Vandana Zaman,Kelsey P Drasites,Denise Matzelle,Sushant Sawant,Alexey Vertegel,Abhay Varma,Naren L Banik

Frontiers in pharmacology 15:1290128 PubMed38384299

2024

Neuroprotective effects of a novel peptide through the Rho-integrin-Tie2 and PI3K/Akt pathways in experimental autoimmune encephalomyelitis model.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Han Qu,Xiao-Xiao Fu,Miao-Miao Xu,Qiang Li,Yuan Jiang,Shu Han

Neural regeneration research 18:1990-1998 PubMed36926724

2023

Low-temperature 3D-printed collagen/chitosan scaffolds loaded with exosomes derived from neural stem cells pretreated with insulin growth factor-1 enhance neural regeneration after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yin Liu,Yin-He Feng,Qing-Bo Feng,Jian-Yong Zhang,Lin Zhong,Peng Liu,Shan Wang,Yan-Ruo Huang,Xu-Yi Chen,Liang-Xue Zhou

Biomolecules 12: PubMed36551293

2022

Eosinophils Infiltration in Esophageal Muscularis Propria Induces Achalasia-like Esophageal Motility Disorder in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Bin Wang,Lili Zhang,Hong Jin

Autophagy 18:2711-2730 PubMed35263212

2022

RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Peiyi Xiao,Jinmin Gu,Wei Xu,Xingyang Niu,Jian Zhang,Jingjing Li,Yicong Chen,Zhong Pei,Jinsheng Zeng,Shihui Xing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

Western blot - Anti-Myelin Basic Protein antibody [MBP2] - Oligodendrocyte Marker (AB11159)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise