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AB249911

Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Myelin expression factor 2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

KIAA1341, MYEF2, Myelin expression factor 2, MEF-2, MyEF-2, MST156

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)

This data was developed using ab176335, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)

This data was developed using ab176335, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of SH-SY5Y cells labeling Myelin expression factor 2 with ab176335 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)

This data was developed using ab176335, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)
  • WB

Supplier Data

Western blot - Anti-Myelin expression factor 2 antibody [EPR10775(B)] - BSA and Azide free (AB249911)

This data was developed using ab176335, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (<a href='/en-us/products/primary-antibodies/myelin-expression-factor-2-antibody-epr10775b-ab176335'>ab176335</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

MCF7 lysate at 10 µg

Lane 4:

fetal brain lysate at 10 µg

Lane 5:

fetal liver lysate at 10 µg

Lane 6:

Raw 264.7 lysate at 10 µg

Predicted band size: 64 kDa

false

  • Unconjugated

    Anti-Myelin expression factor 2 antibody [EPR10775(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10775(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Anti-TSG101 antibody [EPR7130(B)]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab249911 is the carrier-free version of ab176335.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin expression factor 2 also known as MYEF2 is a transcription repressor that regulates the transcription of myelin-specific genes. It plays an important role in the control of myelin production and is primarily expressed in the nervous system especially in oligodendrocytes. The factor weighs approximately 100 kDa and its expression is critical at the periods of neuronal development. MYEF2 demonstrates its activity within the cell nucleus where it functions directly on DNA to modulate gene expression.
Biological function summary

MYEF2 influences the differentiation and maturation of oligodendrocytes. These cells are responsible for forming the myelin sheath in the central nervous system which is essential for rapid impulse conduction along nerve fibers. MYEF2 does not act alone but integrates into complexes with other proteins such as histone deacetylases to exert its repressive function. This interaction fine-tunes oligodendrocyte activity ensuring proper neuronal insulation and function.

Pathways

MYEF2 interacts with the signaling cascades of myelination and neural development. In myelination pathways MYEF2 collaborates closely with proteins like SOX10 and OLIG1 both of which are vital for oligodendrocyte differentiation and maturation. Additionally MYEF2 associates indirectly with the Notch pathway affecting neural progenitor cell fate decisions. The integration of these pathways guarantees the precise regulation of myelin gene expression and hence normal nervous system function.

MYEF2's dysregulation links to demyelinating diseases such as multiple sclerosis and certain leukodystrophies. In multiple sclerosis improper MYEF2 activity can lead to defective myelin production exacerbating disease symptoms and progression. MYEF2's function could also relate to disturbances in proteins like myelin basic protein (MBP) which is important for myelin integrity. Understanding MYEF2’s role in these disorders may offer insights for therapeutic targets to manage and treat neurological conditions involving myelin damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA (By similarity).
See full target information MYEF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com