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Rabbit Recombinant Monoclonal Myelin oligodendrocyte glycoprotein antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 18 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (AB109746), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (AB109746), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (AB109746), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (AB109746), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (AB109746), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

In Western blot analysis, ab109746 exhibited lower sensitivity compared to Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505 and Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab233549, for which reason we recommend the latter two as superior alternatives.

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if ab109746 must be used.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

In Western blot analysis, ab109746 exhibited lower sensitivity compared to Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505 and Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab233549, for which reason we recommend the latter two as superior alternatives.

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if ab109746 must be used.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. (Microbial infection) Acts as a receptor for rubella virus.

Alternative names

Myelin-oligodendrocyte glycoprotein, MOG

Recommended products

Rabbit Recombinant Monoclonal Myelin oligodendrocyte glycoprotein antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 18 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP4281
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Myelin oligodendrocyte glycoprotein (MOG) also known as MOG protein or MOG glycoprotein is a lesser-known but important component of the central nervous system myelin. The MOG protein has a molecular mass of approximately 26 to 28 kDa. You will find it expressed on the surface of myelin sheaths and oligodendrocytes. This protein plays a role in the myelination process acting as a potential adhesive molecule or signaling molecule contributing to the stability and integrity of the myelin structure. Oligodendrocyte staining techniques can help visualize the distribution and expression patterns of MOG making it important for research purposes.

Biological function summary

MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.

Pathways

MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.

Associated diseases and disorders

MOG plays a critical role in conditions such as multiple sclerosis and neuromyelitis optica. In multiple sclerosis MOG is a target of autoantibodies leading to demyelination and loss of neural function. In neuromyelitis optica MOG antibodies may contribute to severe inflammation and damage in optic nerves and spinal cord. The connection between MOG and these diseases highlights associations with other proteins like aquaporin-4 which are similarly targeted in associated autoimmune responses. Understanding these relationships is key for developing diagnostics or therapies involving MOG ELISA methods to detect MOG-specific antibodies in patient samples.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    ab109746 showing negative staining in Breast carcinoma tissue. This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    ab109746 showing negative staining in Normal breast tissue. This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    ab109746 showing negative staining in Colonic adenocarcinoma tissue. This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    ab109746 showing negative staining in Normal tonsil tissue. This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    ab109746 showing negative staining in Normal colon tissue. This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    ab109746 showing negative staining in Benign prostatic hyperplasia tissue. This image was produced using unpurified antibody.

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Blocking buffer concentrations: 5% NFDM/TBST

    Diluting buffer concentrations: 5% NFDM/TBST

    Exposure equipment: iBright CL 1000 Imaging System

    All lanes: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/1000 dilution

    All lanes: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa

    Observed band size: 25 kDa, 15 kDa

    Exposure time: 3s

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Exposure time: Lane 1-3&5-7:20 seconds; Lane 4&8: 180 seconds

    In Western blot analysis, ab109746 exhibited lower sensitivity compared to Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505 and Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab233549, for which reason we recommend the latter two as superior alternatives

    Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if ab109746 must be used.

    Lanes 1, 4, 5 and 8: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/1000 dilution

    Lanes 2 and 6: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] (Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505) at 1/1000 dilution

    Lanes 3 and 7: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab233549) at 1/1000 dilution

    Lanes 1 - 4: Mouse brain tissue lysate at 20 µg

    Lanes 5 - 8: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 25 kDa

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The bands beneath the target band (25 kDa) are likely to be degraded target fragments.

    In lanes 1-2, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In lanes 3-4, this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Exposure time:

    Lanes 1-2: 180 seconds,

    Lanes 3-4: 92 seconds.

    Exposure equipment: iBright CL 1000 Imaging System.

    All lanes: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/1000 dilution

    Lanes 1 and 3: Mouse brain tissue lysate at 20 µg

    Lanes 2 and 4: Rat brain tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Lanes 3 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa

    Observed band size: 25 kDa, 15 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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