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Rabbit Recombinant Monoclonal Myelin oligodendrocyte glycoprotein antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Human
Expected
Tested
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.(Microbial infection) Acts as a receptor for rubella virus.

Alternative names

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Rabbit Recombinant Monoclonal Myelin oligodendrocyte glycoprotein antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22629-310

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.

Activity summary

Myelin oligodendrocyte glycoprotein (MOG) also known as MOG protein or MOG glycoprotein is a lesser-known but important component of the central nervous system myelin. The MOG protein has a molecular mass of approximately 26 to 28 kDa. You will find it expressed on the surface of myelin sheaths and oligodendrocytes. This protein plays a role in the myelination process acting as a potential adhesive molecule or signaling molecule contributing to the stability and integrity of the myelin structure. Oligodendrocyte staining techniques can help visualize the distribution and expression patterns of MOG making it important for research purposes.

Pathways

MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.

Associated diseases and disorders

MOG plays a critical role in conditions such as multiple sclerosis and neuromyelitis optica. In multiple sclerosis MOG is a target of autoantibodies leading to demyelination and loss of neural function. In neuromyelitis optica MOG antibodies may contribute to severe inflammation and damage in optic nerves and spinal cord. The connection between MOG and these diseases highlights associations with other proteins like aquaporin-4 which are similarly targeted in associated autoimmune responses. Understanding these relationships is key for developing diagnostics or therapies involving MOG ELISA methods to detect MOG-specific antibodies in patient samples.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 μg (Input).
    Lane 2: ab233549 IP in mouse brain tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233549 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549)

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 26 seconds; Lane 2: 10 seconds.

    All lanes: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549) at 1/1000 dilution

    All lanes: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

    Exposure time: 3min

  • Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of mouse cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of human cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

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Product protocols

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