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Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) is a rabbit monoclonal antibody that is used to detect Myelin oligodendrocyte glycoprotein in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549), expandable thumbnail
  • Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549), expandable thumbnail
  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549), expandable thumbnail
  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB233549), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-FrIHC-P
Human
Tested
Expected
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Rat
Tested
Expected
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

In Western blot analysis, Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 exhibited lower sensitivity compared to Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505 and ab233549, for which reason we recommend the latter two as superior alternatives.

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 must be used.

Species
Rat
Dilution info
1/1000
Notes

In Western blot analysis, Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 exhibited lower sensitivity compared to Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505 and ab233549, for which reason we recommend the latter two as superior alternatives.

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 must be used.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
1/500
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. (Microbial infection) Acts as a receptor for rubella virus.

Alternative names

Recommended products

Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) is a rabbit monoclonal antibody that is used to detect Myelin oligodendrocyte glycoprotein in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22629-310
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Human, Mouse, Rat samples.

What is the molecular weight of Myelin oligodendrocyte glycoprotein?


Anti-Myelin oligodendrocyte glycoprotein [EPR22629-310] (ab233549) specifically detects a band for Myelin oligodendrocyte glycoprotein (UniProt: Q61885) at a molecular weight of 28kDa.

Recommended positive controls


WB: Human brain tissue lysate; mouse brain tissue lysate; rat brain tissue lysate.IP: Mouse brain tissue lysate.IHC-P: Human cerebellum tissue; mouse cerebellum tissue; rat cerebellum tissue.IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR22629-310] also available for your convenience:
ab233549, Carrier free - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free ab255266, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab305372, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab306598, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab306602, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab307127



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Myelin oligodendrocyte glycoprotein (MOG) also known as MOG protein or MOG glycoprotein is a lesser-known but important component of the central nervous system myelin. The MOG protein has a molecular mass of approximately 26 to 28 kDa. You will find it expressed on the surface of myelin sheaths and oligodendrocytes. This protein plays a role in the myelination process acting as a potential adhesive molecule or signaling molecule contributing to the stability and integrity of the myelin structure. Oligodendrocyte staining techniques can help visualize the distribution and expression patterns of MOG making it important for research purposes.

Biological function summary

MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.

Pathways

MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.

Associated diseases and disorders

MOG plays a critical role in conditions such as multiple sclerosis and neuromyelitis optica. In multiple sclerosis MOG is a target of autoantibodies leading to demyelination and loss of neural function. In neuromyelitis optica MOG antibodies may contribute to severe inflammation and damage in optic nerves and spinal cord. The connection between MOG and these diseases highlights associations with other proteins like aquaporin-4 which are similarly targeted in associated autoimmune responses. Understanding these relationships is key for developing diagnostics or therapies involving MOG ELISA methods to detect MOG-specific antibodies in patient samples.

Product promise

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9 product images

  • Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 μg (Input).
    Lane 2: ab233549 IP in mouse brain tissue lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab233549 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 26 seconds; Lane 2: 10 seconds.

    All lanes: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on white matter of rat cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution

    All lanes: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

    Exposure time: 3min

  • Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on white matter of mouse cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on white matter of human cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    The section was incubated with ab233549 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549), expandable thumbnail

    Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

    Exposure time: Lane 1-3&5-7:20 seconds; Lane 4&8: 180 seconds

    In Western blot analysis, Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 exhibited lower sensitivity compared to Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505 and ab233549, for which reason we recommend the latter two as superior alternatives

    Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 must be used.

    Lanes 1, 4, 5 and 8: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746) at 1/1000 dilution

    Lanes 2 and 6: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] (Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] ab108505) at 1/1000 dilution

    Lanes 3 and 7: Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution

    Lanes 1 - 4: Mouse brain tissue lysate at 20 µg

    Lanes 5 - 8: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 25 kDa

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