Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of human cerebellum (PMID : 25421634) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab233549 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233549).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of mouse cerebellum (PMID : 25421634) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab233549 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233549).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID : 26347141) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233549).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of rat cerebellum (PMID : 25421634) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab233549 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233549).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID : 26347141) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233549).

• IP

Unknown

Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 μg (Input).
Lane 2 : ab233549 IP in mouse brain tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233549 in mouse brain tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233549).

All lanes:

Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-oligodendrocyte-marker-ab233549'>ab233549</a>)

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

• WB

Lab

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free (AB255266)

This data was developed using ab233549, the same antibody clone in a different buffer formulation.

Exposure time : Lane 1-3&5-7 : 20 seconds; Lane 4&8 : 180 seconds

In Western blot analysis, ab109746 exhibited lower sensitivity compared to ab108505 and ab233549, for which reason we recommend the latter two as superior alternatives

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1 : 500), and fg-grade ECL substrates are suggested if ab109746 must be used.

Lanes 1, 4, 5 and 8:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-ep4281-oligodendrocyte-marker-ab109746'>ab109746</a>) at 1/1000 dilution

Lanes 2 and 6:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-epr4282-oligodendrocyte-marker-ab108505'>ab108505</a>) at 1/1000 dilution

Lanes 3 and 7:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-oligodendrocyte-marker-ab233549'>ab233549</a>) at 1/1000 dilution

Lanes 1 - 4:

Mouse brain tissue lysate at 20 µg

Lanes 5 - 8:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25 kDa

false