Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker
- BOND RX™ Validated
- RabMAb
- Recombinant
- What is this?
5
(3 Reviews)
|
(6 Publications)
Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) is a rabbit monoclonal antibody detecting Myelin oligodendrocyte glycoprotein in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
Myelin-oligodendrocyte glycoprotein, MOG
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of human cerebellum (PMID : 25421634) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab233549 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of mouse cerebellum (PMID : 25421634) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab233549 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID : 26347141) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of rat cerebellum (PMID : 25421634) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab233549 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID : 26347141) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
- IP
Unknown
Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1 : Mouse brain tissue lysate 10 μg (Input).
Lane 2 : ab233549 IP in mouse brain tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233549 in mouse brain tissue lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.
All lanes:
Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (ab233549)
Predicted band size: 28 kDa
Observed band size: 28 kDa
false
- WB
Unknown
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (ab233549) at 1/1000 dilution
All lanes:
Human brain tissue lysate at 20 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
false
Exposure time: 3min
- WB
Lab
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Exposure time : Lane 1-3&5-7 : 20 seconds; Lane 4&8 : 180 seconds
In Western blot analysis, ab109746 exhibited lower sensitivity compared to ab108505 and ab233549, for which reason we recommend the latter two as superior alternatives
Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1 : 500), and fg-grade ECL substrates are suggested if ab109746 must be used.
Lanes 1, 4, 5 and 8:
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-ep4281-oligodendrocyte-marker-ab109746'>ab109746</a>) at 1/1000 dilution
Lanes 2 and 6:
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-epr4282-oligodendrocyte-marker-ab108505'>ab108505</a>) at 1/1000 dilution
Lanes 3 and 7:
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (ab233549) at 1/1000 dilution
Lanes 1 - 4:
Mouse brain tissue lysate at 20 µg
Lanes 5 - 8:
Rat brain tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 25 kDa
false
- WB
Unknown
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (AB233549)
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure times.
Lane 1 : 26 seconds; Lane 2 : 10 seconds.
All lanes:
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (ab233549) at 1/1000 dilution
Lane 1:
Mouse brain tissue lysate at 20 µg
Lane 2:
Rat brain tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
false
Related conjugates and formulations (6)
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Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - BSA and Azide free
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker
Reactivity data
Product details
What is this antibody validated in?
Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of Myelin oligodendrocyte glycoprotein?
Anti-Myelin oligodendrocyte glycoprotein [EPR22629-310] (ab233549) specifically detects a band for Myelin oligodendrocyte glycoprotein (UniProt: Q61885) at a molecular weight of 28kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR22629-310] also available for your convenience: ab233549, Carrier free - ab255266, Alexa Fluor® 594 - ab305372, Alexa Fluor® 647 - ab306598, Alexa Fluor® 488 - ab306602, Alexa Fluor® 555 - ab307127
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.
Pathways
MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.
Product protocols
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Target data
Publications (6)
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Molecular psychiatry : PubMed40993340
2025
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Nature methods 22:1911-1922 PubMed40954300
2025
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Frontiers in pharmacology 16:1590624 PubMed40843375
2025
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Signal transduction and targeted therapy 10:167 PubMed40425583
2025
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Glia 73:1452-1466 PubMed40105030
2025
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Oxidative medicine and cellular longevity 2022:3800004 PubMed36092158
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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