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AB105784

Anti-Myelin PLP antibody

5

(10 Reviews)

|

(18 Publications)

Rabbit Polyclonal Myelin PLP antibody. Suitable for WB, IHC-Fr and reacts with Rat, Mouse samples. Cited in 18 publications.

View Alternative Names

Plp, Plp1, Myelin proteolipid protein, PLP, Lipophilin

2 Images
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody (AB105784)
  • IHC-Fr

AbReview35537****

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody (AB105784)

IHC-Fr image of myelin PLP staining on Mouse sciatic nerve sections using ab105784 (1 : 3000). Fresh frozen sections (10μM) and dried in fumehood for 2 hours before fixing with 10% formalin for 10 min. Sections were permeablized in 0.1% Triton X-100 in TBS for 10min. Sections were then blocked using 1% BSA for 10 mins at 21°C. ab105784 was diluted 1 : 2000 in TBS buffer (containing BSA) and sections were then incubated with ab105784 for 16 hours at 21°C. The secondary antibody used was Alexa Fluor® 594 conjugated Goat polyclonal to Rabbit IgG (1 : 1000).

Image courtesy of Carl Hobbs (Imperial College London, United Kingdom)

Western blot - Anti-Myelin PLP antibody (AB105784)
  • WB

Unknown

Western blot - Anti-Myelin PLP antibody (AB105784)

All lanes:

Western blot - Anti-Myelin PLP antibody (ab105784) at 1 µg/mL

Lane 1:

Brain (Rat) Tissue Lysate at 10 µg

Lane 2:

Spinal Cord (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 30 kDa

Observed band size: 34 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Common marmoset": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin proteolipid protein or PLP is an integral membrane protein with important roles in the central nervous system. It has an alternate name lipophilin and its molecular weight is approximately 25 kDa. PLP is most abundant in myelin the protective sheath covering nerve fibers and is expressed in oligodendrocytes and Schwann cells. It represents one of the major protein components of central nervous system myelin and facilitates the compact structure of myelin via its structural properties.
Biological function summary

The myelin proteolipid protein contributes to the stability and functionality of the myelin sheath impacting nervous impulse conduction. It plays a structural role in the formation and maintenance of myelin which is essential for rapid signal transmission in the nervous system. PLP can form a complex with DM20 a related isoform which aids in the regulatory processes of myelination. The protein is critical for the compaction and shear resistance of the myelin sheath ensuring efficient neuronal communication.

Pathways

The myelin proteolipid protein is involved in myelination and cell adhesion processes that are part of central nervous system development. It participates in the myelin assembly pathway and interacts with other key proteins like 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) to modulate myelin biogenesis and homeostasis. This interaction is important for the formation and repair of myelin ensuring that signal transduction remains unobstructed in the nervous system.

Mutations or misregulations of the myelin proteolipid protein are linked to conditions such as Pelizaeus-Merzbacher disease (PMD) and multiple sclerosis (MS). In PMD altered PLP expression leads to abnormal myelin formation resulting in severe neurological dysfunction. In cases of MS the autoimmune response may involve components of the myelin such as PLP initiating demyelination and impairing nerve conductivity. Other proteins connected to these disorders include myelin basic protein (MBP) which alongside PLP forms the bulk of myelin structure and both are targets in autoimmune attack scenarios.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
See full target information Plp1
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:75 PubMed39824932

2025

Prenatal inflammation impairs early CD11c-positive microglia induction and delays myelination in neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Fuma,Yukako Iitani,Kenji Imai,Takafumi Ushida,Sho Tano,Kosuke Yoshida,Akira Yokoi,Rika Miki,Hiroyuki Kidokoro,Yoshiaki Sato,Yuichiro Hara,Tomoo Ogi,Kohei Nomaki,Makoto Tsuda,Okiru Komine,Koji Yamanaka,Hiroaki Kajiyama,Tomomi Kotani

Translational psychiatry 14:68 PubMed38296969

2024

Amelioration of the brain structural connectivity is accompanied with changes of gut microbiota in a tuberous sclerosis complex mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Chin-Jung Hsieh,Yu-Chun Lo,Hsin-Hui Wang,Hsin-Ying Shen,You-Yin Chen,Yi-Chao Lee

Neural plasticity 2023:8938674 PubMed37006814

2023

Deficiency of MicroRNA-23-27-24 Clusters Exhibits the Impairment of Myelination in the Central Nervous System.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Tsuchikawa,Naosuke Kamei,Yohei Sanada,Toshio Nakamae,Takahiro Harada,Kazunori Imaizumi,Takayuki Akimoto,Shigeru Miyaki,Nobuo Adachi

Heliyon 9:e14361 PubMed36938412

2023

Development of a new surgical technique to infuse kynurenic acid to optic nerves in chickens for studying loss of myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Akshay Gurdita,Jacek M Kwiecien,Vivian Choh

Molecular neurobiology 60:1675-1689 PubMed36550333

2022

A Disintegrin and Metalloproteinase 10 (ADAM10) Is Essential for Oligodendrocyte Precursor Development and Myelination in the Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Dazhi Guo,Fei Huang,Ruijun Xue,Yuehong Ma,Lin Xiao,Huifang Lou,Shuyi Pan

The Journal of experimental medicine 219: PubMed34846535

2021

Protection of ischemic white matter and oligodendrocytes in mice by 3K3A-activated protein C.

Applications

Unspecified application

Species

Unspecified reactive species

Mikko T Huuskonen,Yaoming Wang,Angeliki Maria Nikolakopoulou,Axel Montagne,Zhonghua Dai,Divna Lazic,Abhay P Sagare,Zhen Zhao,Jose A Fernandez,John H Griffin,Berislav V Zlokovic

eLife 10: PubMed33942715

2021

Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Seula Shin,Chenxi He,Qiang Zhang,Matthew N Rasband,Jiangong Ren,Congxin Dai,Rocío I Zorrilla-Veloz,Takashi Shingu,Liang Yuan,Yunfei Wang,Yiwen Chen,Fei Lan,Jian Hu

Lipids 55:627-637 PubMed32537944

2020

Myelin and Lipid Composition of the Corpus Callosum in Mucopolysaccharidosis Type I Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Steven Q Le,Igor Nestrasil,Shih-Hsin Kan,Martin Egeland,Jonathan D Cooper,David Elashoff,Rong Guo,Jakub Tolar,Jennifer K Yee,Patricia I Dickson

The Journal of veterinary medical science 82:827-835 PubMed32321871

2020

Immunohistochemical analysis for acetylcholinesterase and choline acetyltransferase in mouse cerebral cortex after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoyo Horio,Aisa Ozawa,Junichi Kamiie,Motoharu Sakaue

The Journal of clinical investigation 130:2220-2236 PubMed32202512

2020

Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα-mediated lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Chenxi He,Jiangong Ren,Congxin Dai,Sharon R Stevens,Qianghu Wang,Daniel Zamler,Takashi Shingu,Liang Yuan,Chythra R Chandregowda,Yunfei Wang,Visweswaran Ravikumar,Arvind Uk Rao,Feng Zhou,Hongwu Zheng,Matthew N Rasband,Yiwen Chen,Fei Lan,Amy B Heimberger,Benjamin M Segal,Jian Hu
View all publications
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