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AB250654

Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Myelin PLP antibody. Carrier free. Suitable for IP, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

PLP, PLP1, Myelin proteolipid protein, Lipophilin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (AB250654)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (AB250654)

This data was developed using ab183493, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Myelin PLP with ab183493 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (AB250654)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (AB250654)

This data was developed using ab183493, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Myelin PLP with ab183493 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (AB250654)
  • IP

Supplier Data

Immunoprecipitation - Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (AB250654)

This data was developed using ab183493, the same antibody clone in a different buffer formulation.

Western blot analysis of Myelin PLP in Mouse brain lysate immunoprecipitated using ab183493 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of mouse brain lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Myelin PLP antibody [EPR12675(2)(B)] (<a href='/en-us/products/primary-antibodies/myelin-plp-antibody-epr126752b-ab183493'>ab183493</a>)

Predicted band size: 30 kDa

false

  • Unconjugated

    Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 578 PE

    PE Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 660 APC

    APC Anti-Myelin PLP antibody [EPR12675(2)(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Myelin PLP antibody [EPR12675(2)(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12675(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250654 is the carrier-free version of ab183493.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin proteolipid protein or PLP is an integral membrane protein with important roles in the central nervous system. It has an alternate name lipophilin and its molecular weight is approximately 25 kDa. PLP is most abundant in myelin the protective sheath covering nerve fibers and is expressed in oligodendrocytes and Schwann cells. It represents one of the major protein components of central nervous system myelin and facilitates the compact structure of myelin via its structural properties.
Biological function summary

The myelin proteolipid protein contributes to the stability and functionality of the myelin sheath impacting nervous impulse conduction. It plays a structural role in the formation and maintenance of myelin which is essential for rapid signal transmission in the nervous system. PLP can form a complex with DM20 a related isoform which aids in the regulatory processes of myelination. The protein is critical for the compaction and shear resistance of the myelin sheath ensuring efficient neuronal communication.

Pathways

The myelin proteolipid protein is involved in myelination and cell adhesion processes that are part of central nervous system development. It participates in the myelin assembly pathway and interacts with other key proteins like 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) to modulate myelin biogenesis and homeostasis. This interaction is important for the formation and repair of myelin ensuring that signal transduction remains unobstructed in the nervous system.

Mutations or misregulations of the myelin proteolipid protein are linked to conditions such as Pelizaeus-Merzbacher disease (PMD) and multiple sclerosis (MS). In PMD altered PLP expression leads to abnormal myelin formation resulting in severe neurological dysfunction. In cases of MS the autoimmune response may involve components of the myelin such as PLP initiating demyelination and impairing nerve conductivity. Other proteins connected to these disorders include myelin basic protein (MBP) which alongside PLP forms the bulk of myelin structure and both are targets in autoimmune attack scenarios.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
See full target information PLP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 33:451-462 PubMed35775321

2022

Notoginsenoside R1 alleviates spinal cord injury by inhibiting oxidative stress, neuronal apoptosis, and inflammation via activating the nuclear factor erythroid 2 related factor 2/heme oxygenase-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Luo,Zhangli Bao,Mingjian Zhou,Yuxin Chen,Zhaoxi Huang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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