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AB254363

Anti-Myelin PLP antibody [EPR23504-106]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Advanced Validation
  • Recombinant
  • What is this?

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(13 Publications)

Anti-Myelin PLP antibody [EPR23504-106] (ab254363) is a rabbit monoclonal antibody detecting Myelin PLP in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, mIHC. Suitable for Human, Mouse, Rat.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

PLP, PLP1, Myelin proteolipid protein, Lipophilin

16 Images
Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-P2Y12 (ab254347, red; Opal™570) on human cerebrum. Panel B : anti-P2Y12 stained on microglial cells. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Fluorescence multiplex immunohistochemical analysis of the human cerebellum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-P2Y12 (ab254347 red; Opal™570) on human cerebellum. Panel B : anti-P2Y12 stained on microglial cells. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Myelin PLP with ab254363 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID : 29081415). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Fluorescence multiplex immunohistochemical analysis of human cerebrum tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-MAP2 (ab254263, red; Opal™570) on human cerebrum tissue. Panel B : anti-MAP2 stained cell body and dendrites of neurons. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND®RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254263 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

Image acquisition was performed with Leica SP8 confocal microscope.

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Fluorescence multiplex immunohistochemical analysis of human cerebellum tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-MAP2 (ab254263, red; Opal™570) on human cerebellum tissue. Panel B : anti-MAP2 stained cell body and dendrites of neurons. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND®RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254263 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab254363 at 1/500 (1.042 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Myelin PLP with ab254363 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Myelin PLP with ab254363 at 1/500 (1.042 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Myelin PLP with ab254363 at 1/500 (1.042 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Myelin PLP with ab254363 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID : 28066178). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunocytochemistry analysis of mouse primary neural/glia cells labelling Myelin PLP with ab254363 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 mouse monoclonal antibody (ab11267) at 1/200 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Confocal image showing cytoplasmic staining in mouse primary glia cells.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunocytochemistry analysis of rat primary neural/glia cells labelling Myelin PLP with ab254363 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Myelin Basic Protein rat monoclonal antibody at 1/100 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Confocal image showing cytoplasmic staining in rat primary glia cells.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab254363 at 1/500 (1.042 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • IP

Unknown

Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Myelin PLP was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab254363 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 5 ug

Lane 2 : ab254363 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254363 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 6 seconds.

All lanes:

Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] (ab254363)

Predicted band size: 30 kDa

Observed band size: 20 kDa,23 kDa

false

Western blot - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • WB

Lab

Western blot - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9247276)

Exposure time : 1 second.

All lanes:

Western blot - Anti-Myelin PLP antibody [EPR23504-106] (ab254363) at 1/5000 dilution

All lanes:

Human brain tissue lysate at 10 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 20 kDa,23 kDa

false

Western blot - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)
  • WB

Lab

Western blot - Anti-Myelin PLP antibody [EPR23504-106] (AB254363)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9247276).

Negative control : liver (PMID : 2414013).

Exposure time : 37 seconds.

All lanes:

Western blot - Anti-Myelin PLP antibody [EPR23504-106] (ab254363) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 30 kDa

Observed band size: 20 kDa,23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23504-106

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, IP, IHC-Fr, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Myelin PLP antibody [EPR23504-106] (ab254363) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

What is the molecular weight of Myelin PLP?
Anti-Myelin PLP [EPR23504-106] (ab254363) specifically detects a band for Myelin PLP (UniProt: P60201) at a molecular weight of 30kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23504-106] also available for your convenience: ab254363, Carrier free - ab275751, Alexa Fluor® 594 - ab311737, Alexa Fluor® 568 - ab313016, Alexa Fluor® 555 - ab313220, Alexa Fluor® 488 - ab319182, Alexa Fluor® 750 - ab321506

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin proteolipid protein or PLP is an integral membrane protein with important roles in the central nervous system. It has an alternate name lipophilin and its molecular weight is approximately 25 kDa. PLP is most abundant in myelin the protective sheath covering nerve fibers and is expressed in oligodendrocytes and Schwann cells. It represents one of the major protein components of central nervous system myelin and facilitates the compact structure of myelin via its structural properties.
Biological function summary

The myelin proteolipid protein contributes to the stability and functionality of the myelin sheath impacting nervous impulse conduction. It plays a structural role in the formation and maintenance of myelin which is essential for rapid signal transmission in the nervous system. PLP can form a complex with DM20 a related isoform which aids in the regulatory processes of myelination. The protein is critical for the compaction and shear resistance of the myelin sheath ensuring efficient neuronal communication.

Pathways

The myelin proteolipid protein is involved in myelination and cell adhesion processes that are part of central nervous system development. It participates in the myelin assembly pathway and interacts with other key proteins like 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) to modulate myelin biogenesis and homeostasis. This interaction is important for the formation and repair of myelin ensuring that signal transduction remains unobstructed in the nervous system.

Mutations or misregulations of the myelin proteolipid protein are linked to conditions such as Pelizaeus-Merzbacher disease (PMD) and multiple sclerosis (MS). In PMD altered PLP expression leads to abnormal myelin formation resulting in severe neurological dysfunction. In cases of MS the autoimmune response may involve components of the myelin such as PLP initiating demyelination and impairing nerve conductivity. Other proteins connected to these disorders include myelin basic protein (MBP) which alongside PLP forms the bulk of myelin structure and both are targets in autoimmune attack scenarios.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
See full target information PLP1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular histology 56:313 PubMed40944759

2025

Neuroprotection in neonatal Hypoxia-ischaemia: melatonin targets NCX1 to inhibit mitochondrial autophagy via the PINK1-Parkin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tongfei Cheng,Shanlong Du,Yi Cao,Ziyan Lu,Yingjun Xu

Acta neuropathologica 150:23 PubMed40906043

2025

TDP-43 dysregulation impairs cholesterol metabolism linked with myelination defects.

Applications

Unspecified application

Species

Unspecified reactive species

Irene García-Toledo,Juan M Godoy-Corchuelo,Luis C Fernández-Beltrán,Zeinab Ali,Ariadna Guindo-Arroyo,Irene Jiménez-Coca,Jesús Jiménez-Rodríguez,Karen Javaloyes-García,Marcos Viñuela,Ulises Gómez-Pinedo,Laura Saiz-Aúz,Alberto Rábano,Estela Área-Gómez,Thomas J Cunningham,Silvia Corrochano

Nature communications 16:7338 PubMed40781079

2025

Early Locus Coeruleus noradrenergic axon loss drives olfactory dysfunction in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Meyer,Theresa Niedermeier,Paul L C Feyen,Felix L Strübing,Boris-Stephan Rauchmann,Katerina Karali,Johanna Gentz,Yannik E Tillmann,Nicolas F Landgraf,Svenja-Lotta Rumpf,Katharina Ochs,Karin Wind-Mark,Gloria Biechele,Jessica Wagner,Selim Guersel,Carolin I Kurz,Meike Schweiger,Danilo Prtvar,Yuan Shi,Richard B Banati,Guo-Jun Liu,Ryan J Middleton,Gerda Mitteregger-Kretzschmar,Robert Perneczky,Thomas Koeglsperger,Jonas J Neher,Sabina Tahirovic,Matthias Brendel,Jochen Herms,Lars Paeger

ASN neuro 17:2535963 PubMed40692140

2025

Identifying Lanthionine Ketimine Derivatives for Maturation and Proliferative Effects in Oligodendrocyte Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary McDonald,Ankit Tandon,Travis T Denton,Mehek Taneja,Jacqueline Rocha,Jeffrey L Dupree,Pablo M Paez,Veronica T Cheli,Swathi G Tumuluri,Douglas L Feinstein

Theranostics 15:4763-4784 PubMed40225581

2025

Microglial Lcn2 knockout enhances chronic intracerebral hemorrhage recovery by restoring myelin and reducing inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Lei Zhang,Kai Wang,Jun He,Liang Yuan,Yangang Wang,Weihao Lv,Zehan Zhang,Yuan Feng,Hongchen Zhang,Min Zhang,Rui Lv,Ya-Nan Dou,Xiaowei Fei,Jialiang Wei

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 44:1487-1500 PubMed38853430

2024

Agomelatine promotes differentiation of oligodendrocyte precursor cells and preserves white matter integrity after cerebral ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shisi Wang,Chunyi Li,Xinmei Kang,Xiaotao Su,Yuxin Liu,Yuge Wang,Sanxin Liu,Xiaohui Deng,Huipeng Huang,Tiemei Li,Danli Lu,Wei Cai,Zhengqi Lu,Lei Wei,Tingting Lu

Endocrine journal 71:233-244 PubMed38233122

2024

Protective effect of alirocumab, a PCSK9 inhibitor, on the sciatic nerve of rats with diabetic peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Na Cui,Yonghao Feng,Ming Wang,Xiuyan Lu,Yongmei Huang,Yinghui Chen,Xiaohong Shi

Nature 619:801-810 PubMed37438528

2023

Spatially resolved multiomics of human cardiac niches.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumasa Kanemaru,James Cranley,Daniele Muraro,Antonio M A Miranda,Siew Yen Ho,Anna Wilbrey-Clark,Jan Patrick Pett,Krzysztof Polanski,Laura Richardson,Monika Litvinukova,Natsuhiko Kumasaka,Yue Qin,Zuzanna Jablonska,Claudia I Semprich,Lukas Mach,Monika Dabrowska,Nathan Richoz,Liam Bolt,Lira Mamanova,Rakeshlal Kapuge,Sam N Barnett,Shani Perera,Carlos Talavera-López,Ilaria Mulas,Krishnaa T Mahbubani,Liz Tuck,Lu Wang,Margaret M Huang,Martin Prete,Sophie Pritchard,John Dark,Kourosh Saeb-Parsy,Minal Patel,Menna R Clatworthy,Norbert Hübner,Rasheda A Chowdhury,Michela Noseda,Sarah A Teichmann

Molecular brain 16:44 PubMed37217969

2023

Comprehensive behavioral analyses of mice with a glycine receptor alpha 4 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Darwish,Satoko Hattori,Hirofumi Nishizono,Tsuyoshi Miyakawa,Nozomu Yachie,Keizo Takao

Frontiers in genetics 13:1045395 PubMed36386836

2022

PLP1 may serve as a potential diagnostic biomarker of uterine fibroids.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Cai,Zhiqi Liao,Shiyu Li,Ruxing Wu,Jie Li,Fang Ren,Hanwang Zhang
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Complementary Products

Primary Antibodies

AB7349

Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker

BOND RX™ Validated|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (AB7349)

  • 4
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Primary Antibodies

AB6319

Anti-CNPase antibody [11-5B]

BOND RX™ Validated|KO Validated

Western blot - Anti-CNPase antibody [11-5B] (AB6319)

  • 5
  • 39
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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