Rabbit Recombinant Monoclonal Myelin PLP antibody. Carrier free. Suitable for ICC/IF, IP, WB, mIHC, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunocytochemistry-immunofluorescence-img94462.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62965.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751)")
![Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--western-blot-img16357.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751)")
![Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunoprecipitation-img3008.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751)")
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunocytochemistry-immunofluorescence-img68757.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (AB275751)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | mIHC | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested | Expected | Tested |
| Mouse | Tested | Tested | Tested | Expected | Tested | Tested |
| Rat | Tested | Expected | Tested | Expected | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
Alternative names
Myelin proteolipid protein, PLP, Lipophilin, PLP, PLP1
Recommended products
Rabbit Recombinant Monoclonal Myelin PLP antibody. Carrier free. Suitable for ICC/IF, IP, WB, mIHC, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.
Alternative names
Myelin proteolipid protein, PLP, Lipophilin, PLP, PLP1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23504-106
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab275751 is the carrier-free version of Anti-Myelin PLP antibody [EPR23504-106] ab254363.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myelin proteolipid protein or PLP is an integral membrane protein with important roles in the central nervous system. It has an alternate name lipophilin and its molecular weight is approximately 25 kDa. PLP is most abundant in myelin the protective sheath covering nerve fibers and is expressed in oligodendrocytes and Schwann cells. It represents one of the major protein components of central nervous system myelin and facilitates the compact structure of myelin via its structural properties.
- Biological function summary
The myelin proteolipid protein contributes to the stability and functionality of the myelin sheath impacting nervous impulse conduction. It plays a structural role in the formation and maintenance of myelin which is essential for rapid signal transmission in the nervous system. PLP can form a complex with DM20 a related isoform which aids in the regulatory processes of myelination. The protein is critical for the compaction and shear resistance of the myelin sheath ensuring efficient neuronal communication.
- Pathways
The myelin proteolipid protein is involved in myelination and cell adhesion processes that are part of central nervous system development. It participates in the myelin assembly pathway and interacts with other key proteins like 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) to modulate myelin biogenesis and homeostasis. This interaction is important for the formation and repair of myelin ensuring that signal transduction remains unobstructed in the nervous system.
- Associated diseases and disorders
Mutations or misregulations of the myelin proteolipid protein are linked to conditions such as Pelizaeus-Merzbacher disease (PMD) and multiple sclerosis (MS). In PMD altered PLP expression leads to abnormal myelin formation resulting in severe neurological dysfunction. In cases of MS the autoimmune response may involve components of the myelin such as PLP initiating demyelination and impairing nerve conductivity. Other proteins connected to these disorders include myelin basic protein (MBP) which alongside PLP forms the bulk of myelin structure and both are targets in autoimmune attack scenarios.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
16 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunocytochemistry-immunofluorescence-img94462.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
Immunocytochemistry analysis of rat primary neural/glia cells labelling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Myelin Basic Protein rat monoclonal antibody at 1/100 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in rat primary glia cells.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62965.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum (PMID: 28066178). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--western-blot-img16357.jpg "Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9247276).
Negative control: liver (PMID: 2414013).
Exposure time: 37 seconds.
All lanes: Western blot - Anti-Myelin PLP antibody [EPR23504-106] (Anti-Myelin PLP antibody [EPR23504-106] ab254363) at 1/2000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 20 kDa, 23 kDa
![Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunoprecipitation-img3008.jpg "Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Myelin PLP was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 5 ug
Lane 2: Anti-Myelin PLP antibody [EPR23504-106] ab254363 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Myelin PLP antibody [EPR23504-106] ab254363 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
All lanes: Immunoprecipitation - Anti-Myelin PLP antibody [EPR23504-106] (Anti-Myelin PLP antibody [EPR23504-106] ab254363)
Predicted band size: 30 kDa
Observed band size: 20 kDa, 23 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunocytochemistry-immunofluorescence-img68757.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
Immunocytochemistry analysis of mouse primary neural/glia cells labelling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 mouse monoclonal antibody (Anti-MAP2 antibody [HM-2] ab11267) at 1/200 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in mouse primary glia cells.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.![Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--western-blot-img29712.jpg "Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9247276)
Exposure time: 1 second.
All lanes: Western blot - Anti-Myelin PLP antibody [EPR23504-106] (Anti-Myelin PLP antibody [EPR23504-106] ab254363) at 1/5000 dilution
All lanes: Human brain tissue lysate at 10 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 20 kDa, 23 kDa
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img98491.jpg "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/500 (1.042 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img1706.jpg "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/500 (1.042 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21277.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img60495.jpg "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/500 (1.042 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img117903.jpg "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/500 (1.042 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104780.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Myelin PLP with Anti-Myelin PLP antibody [EPR23504-106] ab254363 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum (PMID: 29081415). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img403015.jpg "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
Fluorescence multiplex immunohistochemical analysis of the human cerebellum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-GFAP (Anti-GFAP antibody [EPR1034Y] ab68428, gray; Opal™690), anti-Myelin PLP (Anti-Myelin PLP antibody [EPR23504-106] ab254363, green; Opal™520) and anti-P2Y12 (Anti-P2Y12 antibody [EPR23511-72] ab254347 red; Opal™570) on human cerebellum. Panel B: anti-P2Y12 stained on microglial cells. Panel C: anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D: anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428 (1/50 dilution), Anti-Myelin PLP antibody [EPR23504-106] ab254363 (1/2000 dilution), and Anti-P2Y12 antibody [EPR23511-72] ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img403012.jpg "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-GFAP (Anti-GFAP antibody [EPR1034Y] ab68428, gray; Opal™690), anti-Myelin PLP (Anti-Myelin PLP antibody [EPR23504-106] ab254363, green; Opal™520) and anti-P2Y12 (Anti-P2Y12 antibody [EPR23511-72] ab254347, red; Opal™570) on human cerebrum. Panel B: anti-P2Y12 stained on microglial cells. Panel C: anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D: anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428 (1/50 dilution), Anti-Myelin PLP antibody [EPR23504-106] ab254363 (1/2000 dilution), and Anti-P2Y12 antibody [EPR23511-72] ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img402675.jpg "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
Fluorescence multiplex immunohistochemical analysis of human cerebellum tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-GFAP (Anti-GFAP antibody [EPR1034Y] ab68428, gray; Opal™690), anti-Myelin PLP (Anti-Myelin PLP antibody [EPR23504-106] ab254363, green; Opal™520) and anti-MAP2 (Anti-MAP2 antibody [EPR22641-106] ab254263, red; Opal™570) on human cerebellum tissue. Panel B: anti-MAP2 stained cell body and dendrites of neurons. Panel C: anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D: anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND®RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428 (1/50 dilution), Anti-Myelin PLP antibody [EPR23504-106] ab254363 (1/2000 dilution), and Anti-MAP2 antibody [EPR22641-106] ab254263 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img402667.jpg "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)
Fluorescence multiplex immunohistochemical analysis of human cerebrum tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-GFAP (Anti-GFAP antibody [EPR1034Y] ab68428, gray; Opal™690), anti-Myelin PLP (Anti-Myelin PLP antibody [EPR23504-106] ab254363, green; Opal™520) and anti-MAP2 (Anti-MAP2 antibody [EPR22641-106] ab254263, red; Opal™570) on human cerebrum tissue. Panel B: anti-MAP2 stained cell body and dendrites of neurons. Panel C: anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D: anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND®RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428 (1/50 dilution), Anti-Myelin PLP antibody [EPR23504-106] ab254363 (1/2000 dilution), and Anti-MAP2 antibody [EPR22641-106] ab254263 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
The data was developed using Anti-Myelin PLP antibody [EPR23504-106] ab254363, the same antibody clone in a different buffer formulation.
![Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--western-blot-img29712.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img98491.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img1706.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21277.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img60495.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-frozen-sections-img117903.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104780.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img403015.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img403012.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img402675.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
![Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751), expandable thumbnail](https://content.abcam.com/products/images/myelin-plp-antibody-epr23504-106-bsa-and-azide-free-ab275751--multiplex-immunohistochemistry-img402667.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Myelin PLP antibody [EPR23504-106] - BSA and Azide free (ab275751)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com