AB134439

Anti-Myelin Protein Zero antibody

Name: Anti-Myelin Protein Zero antibody (ab134439) | Abcam
Brand: Abcam Limited
SKU: AB134439
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Chicken Polyclonal Myelin Protein Zero antibody. Suitable for WB, IHC-FoFr and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MPZ conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Myelin protein P0, Myelin peripheral protein, Myelin protein zero, MPP, MPZ

3 Images

Unknown

Western blot - Anti-Myelin Protein Zero antibody (AB134439)

All lanes:

Western blot - Anti-Myelin Protein Zero antibody (ab134439) at 1/2000 dilution

All lanes:

Adult mouse (C57 Black) sciatic nerve

Predicted band size: 28 kDa

false

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Protein Zero antibody (AB134439)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Protein Zero antibody (AB134439)

Immunohistochemical analysis of an adult sciatic nerve labelling Myelin Protein Zero in the myelin and Schwann cell processes surrounding the nodes of Ranvier with ab134439 at 1/1000 dilution (green staining). Antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Protein Zero antibody (AB134439)

Immunohistochemical analysis of an adult sciatic nerve labelling Myelin Protein Zero in myelinating Schwann cells with ab134439 at 1/1000 dilution (brown staining).

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Mouse, Human

Applications

IHC-FoFr, WB

applications

Immunogen

Synthetic Peptide within Human MPZ conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25189

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/1000 - 1/2000", "IHCFoFr-species-notes": "<p>Use 2% paraformaldehyde-fixed tissues.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" } } }

style

left-column

Product details

Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin protein zero sometimes called MPZ or P0 is a structural protein in the peripheral nervous system. It has a molecular mass of approximately 30 kDa. Myelin protein zero is mostly found in Schwann cells where it plays an important role in the formation of the myelin sheath. This protein facilitates the adhesion between layers of the myelin membrane contributing to the compact structure of the myelin sheath surrounding nerve fibers.

Biological function summary

Several functions of nerve insulating layers involve myelin protein zero. It forms part of the myelin sheath critical for effective nerve signal transmission. The protein is associated with the Ig superfamily and interacts with other myelin components to maintain integrity and functionality. Myelin protein zero also affects the clustering of specific proteins and lipids within the myelin sheath making it an important component in maintaining the stability and function of peripheral nerves.

Pathways

Several important signal transmission processes involve the interactions of myelin protein zero. It is involved in the myelination pathway which is essential for insulating nerve fibers and speeding up action potential propagation. The protein is closely related to connexin 32 in this pathway another protein involved in maintaining the proper function of myelinated neurons. This coordination helps ensure efficient communication in the peripheral nervous system.

Several neurological conditions link to abnormalities in myelin protein zero. Mutations in the gene encoding this protein can lead to Charcot-Marie-Tooth disease type 1B a hereditary neuropathy characterized by muscle weakness and sensory loss. Alterations in myelin protein zero are also associated with Dejerine-Sottas syndrome a severe neuropathy involving early-onset muscle weakness. The protein's relation to other myelin proteins like PMP22 often signifies its role in the molecular mechanisms underlying these demyelinating disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Is an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.

See full target information MPZ

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 41:e108780 PubMed35815410

2022

Schwann cell precursors represent a neural crest-like state with biased multipotency.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eleni Kastriti,Louis Faure,Dorothea Von Ahsen,Thibault Gerald Bouderlique,Johan Boström,Tatiana Solovieva,Cameron Jackson,Marianne Bronner,Dies Meijer,Saida Hadjab,Francois Lallemend,Alek Erickson,Marketa Kaucka,Viacheslav Dyachuk,Thomas Perlmann,Laura Lahti,Jan Krivanek,Jean-Francois Brunet,Kaj Fried,Igor Adameyko

The Journal of biological chemistry 298:101718 PubMed35151688

2022

Exosomal miR-673-5p from fibroblasts promotes Schwann cell-mediated peripheral neuron myelination by targeting the TSC2/mTORC1/SREBP2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yahong Zhao,Yunyun Liang,Zhixin Xu,Jina Liu,Xiaoyu Liu,Jinyu Ma,Cheng Sun,Yumin Yang

Neuroscience bulletin 37:1542-1554 PubMed34519993

2021

Neuroinflammation Mediates Faster Brachial Plexus Regeneration in Subjects with Cerebral Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Su,Guobao Wang,Tie Li,Su Jiang,Aiping Yu,Xiaomin Wang,Wendong Xu

Health science reports 1:e86 PubMed30623049

2018

A multi-layered nerve guidance conduit design adapted to facilitate surgical implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla Belanger,Guy Schlatter,Anne Hébraud,Frédéric Marin,Sylvie Testelin,Stéphanie Dakpé,Bernard Devauchelle,Christophe Egles

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myelin Protein Zero antibody

Western blot - Anti-Myelin Protein Zero antibody (AB134439)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Protein Zero antibody (AB134439)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Protein Zero antibody (AB134439)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise