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AB183868

Anti-Myelin Protein Zero antibody [EPR20383]

5

(2 Reviews)

|

(16 Publications)

Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) is a rabbit monoclonal antibody detecting Myelin Protein Zero in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Myelin protein P0, Myelin peripheral protein, Myelin protein zero, MPP, MPZ

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunohistochemical analysis of paraffin-embedded human peripheral nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on Schwann cells of human peripheral nerves (PMID : 21057508).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • IP

Lab

Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Myelin Protein Zero was immunoprecipitated from Human Sciatic Nerve lysate with ab183868 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab183868 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Human Sciatic Nerve lysate 10ug (Input).

Lane 2 : ab183868 IP in Human Sciatic Nerve lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab183868 in Human Sciatic Nerve lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)

Predicted band size: 28 kDa

false

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunohistochemical analysis of paraffin-embedded rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on Schwann cells of rat sciatic nerves (PMID : 21057508).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on Schwann cells of mouse sciatic nerves (PMID : 21057508).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunohistochemical analysis of paraffin-embedded mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on Schwann cells of mouse sciatic nerves (PMID : 21057508).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunocytochemistry/ Immunofluorescence analysis of S16 (rat Sciatic nerve epithelial-like) labeling Myelin Protein Zero with ab183868 at 1/100 dilution, followed by ab150081 at 1/1000 dilution. ab195889 at 1/200 was used as the counterstain antibody. Nuclear counterstain was DAPI (blue). Cells were fixed wiht 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Confocal image showing membranous and cytoplasmic staining in S16 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on Schwann cells of rat sciatic nerves (PMID : 21057508).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Immunocytochemistry/ Immunofluorescence analysis of S42 (rat sciatic nerve epithelial-like cell) labeling Myelin Protein Zero with ab183868 at 1/100 dilution, followed by ab150081 at 1/1000 dilution. ab195889 at 1/200 was used as the counterstain antibody. Nuclear counterstain was DAPI (blue). Cells were fixed wiht 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Confocal image showing membranous and cytoplasmic staining in S42 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • WB

Supplier Data

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Blocking/Dilution buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).

Negative control : liver tissue (PMID 2578885).

All lanes:

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/1000 dilution

Lane 1:

Human sciatic nerve lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa,30 kDa,54 kDa

Observed band size: 26-28 kDa,33 kDa,36 kDa,40 kDa,50 kDa

false

Exposure time: 30s

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)
  • WB

Supplier Data

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868)

Blocking/Dilution buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).

Negative control : liver, brain tissue (PMID 2578885, PMID 25288117).

All lanes:

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/5000 dilution

Lane 1:

Mouse sciatic nerve lysate at 4 µg

Lane 2:

Mouse liver lysate at 4 µg

Lane 3:

Mouse brain lysate at 4 µg

Lane 4:

Rat sciatic nerve lysate at 4 µg

Lane 5:

Rat liver lysate at 4 µg

Lane 6:

Rat brain lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 26-28 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-Myelin Protein Zero antibody [EPR20383] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20383

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-Fr, IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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AB134439

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Secondary Antibodies

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Myelin Protein Zero?
Anti-Myelin Protein Zero [EPR20383] (ab183868) specifically detects a band for Myelin Protein Zero (UniProt: P25189) at a molecular weight of 28kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20383] also available for your convenience: ab183868, Carrier free - ab240279

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin protein zero sometimes called MPZ or P0 is a structural protein in the peripheral nervous system. It has a molecular mass of approximately 30 kDa. Myelin protein zero is mostly found in Schwann cells where it plays an important role in the formation of the myelin sheath. This protein facilitates the adhesion between layers of the myelin membrane contributing to the compact structure of the myelin sheath surrounding nerve fibers.
Biological function summary

Several functions of nerve insulating layers involve myelin protein zero. It forms part of the myelin sheath critical for effective nerve signal transmission. The protein is associated with the Ig superfamily and interacts with other myelin components to maintain integrity and functionality. Myelin protein zero also affects the clustering of specific proteins and lipids within the myelin sheath making it an important component in maintaining the stability and function of peripheral nerves.

Pathways

Several important signal transmission processes involve the interactions of myelin protein zero. It is involved in the myelination pathway which is essential for insulating nerve fibers and speeding up action potential propagation. The protein is closely related to connexin 32 in this pathway another protein involved in maintaining the proper function of myelinated neurons. This coordination helps ensure efficient communication in the peripheral nervous system.

Several neurological conditions link to abnormalities in myelin protein zero. Mutations in the gene encoding this protein can lead to Charcot-Marie-Tooth disease type 1B a hereditary neuropathy characterized by muscle weakness and sensory loss. Alterations in myelin protein zero are also associated with Dejerine-Sottas syndrome a severe neuropathy involving early-onset muscle weakness. The protein's relation to other myelin proteins like PMP22 often signifies its role in the molecular mechanisms underlying these demyelinating disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.
See full target information MPZ
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40149554

2025

Reversing Pathology in an Aggravated Fabry Mouse Model Using Low-Dose Engineered Human Alpha-Galactosidase A AAV Gene Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Wanida Ruangsiriluk,Mugdha Deshpande,Natalia Boukharov,Girija Rajarshi,Shreya Mukherji,Shipeng Yuan,Jennifer Wiseman,Nancy Chen,Eric Park,Hyelim Cho,Rizwana Islam

Cell reports 44:115091 PubMed39709602

2024

Transcriptional profile of the rat cardiovascular system at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Arduini,Stephen J Fleming,Ling Xiao,Amelia W Hall,Amer-Denis Akkad,Mark D Chaffin,Kayla J Bendinelli,Nathan R Tucker,Irinna Papangeli,Helene Mantineo,Patricio Flores-Bringas,Mehrtash Babadi,Christian M Stegmann,Guillermo García-Cardeña,Mark E Lindsay,Carla Klattenhoff,Patrick T Ellinor

Cell biology international 48:1698-1713 PubMed39080995

2024

Overexpression of EGFL7 promotes angiogenesis and nerve regeneration in peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zengtao Hao,Zhiqi Huo,Qicheng Aixin-Jueluo,Tao Wu,Yihong Chen

Stem cell research & therapy 15:215 PubMed39020413

2024

Biological characteristics of tissue engineered-nerve grafts enhancing peripheral nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangling Li,Hang Xu,Chaochao Li,Yanjun Guan,Yuli Liu,Tieyuan Zhang,Fanqi Meng,Haofeng Cheng,Xiangyu Song,Zhibo Jia,Ruichao He,Jinjuan Zhao,Shengfeng Chen,Congcong Guan,Shi Yan,Jinpeng Wang,Yu Wei,Jian Zhang,Jinshu Tang,Jiang Peng,Yu Wang

American journal of human genetics 111:309-322 PubMed38272032

2024

CRISPR activation to characterize splice-altering variants in easily accessible cells.

Applications

Unspecified application

Species

Unspecified reactive species

Thorkild Terkelsen,Nanna Steengaard Mikkelsen,Ebbe Norskov Bak,Johan Vad-Nielsen,Jenny Blechingberg,Simone Weiss,Simon Opstrup Drue,Henning Andersen,Brage Storstein Andresen,Rasmus O Bak,Uffe Birk Jensen

JCI insight 9: PubMed38175707

2024

Unbalancing cAMP and Ras/MAPK pathways as a therapeutic strategy for cutaneous neurofibromas.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Mazuelas,Miriam Magallón-Lorenz,Itziar Uriarte-Arrazola,Alejandro Negro,Inma Rosas,Ignacio Blanco,Elisabeth Castellanos,Conxi Lázaro,Bernat Gel,Meritxell Carrió,Eduard Serra

Neuropathology and applied neurobiology 49:e12898 PubMed36868780

2023

Schwann cells and myelin in human peripheral nerve: Major protein components vary with age, axon size and pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Alan Pestronk,Robert E Schmidt,Robert Bucelli,Julia Sim

Neural regeneration research 18:1591-1600 PubMed36571367

2022

The circ_0002538/miR-138-5p/plasmolipin axis regulates Schwann cell migration and myelination in diabetic peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Tian Liu,Zhao Xu,Wei Liu,Sen Ren,He-Wei Xiong,Tao Jiang,Jing Chen,Yu Kang,Qian-Yun Li,Zi-Han Wu,Hans-GüNther Machens,Xiao-Fan Yang,Zhen-Bing Chen

Biological procedures online 24:13 PubMed36117173

2022

Single-cell and WGCNA uncover a prognostic model and potential oncogenes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Di,Sicheng Zhou,Gaoran Xu,Lian Ren,Chengxin Li,Zheyu Ding,Kaixin Huang,Leilei Liang,Yihang Yuan

Frontiers in veterinary science 9:936620 PubMed36046506

2022

Transcription factor SS18L1 regulates the proliferation, migration and differentiation of Schwann cells in peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tianmei Qian,Pingping Qiao,Yingnan Lu,Hongkui Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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