Name: Anti-Myelin Protein Zero antibody [EPR20383]
SKU: ab183868
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal Myelin Protein Zero antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Images

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (AB183868), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-Fr	IHC-P	ICC/IF
Human	Tested	Tested	Expected	Tested	Not recommended
Mouse	Expected	Tested	Tested	Tested	Not recommended
Rat	Expected	Tested	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Rat	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information MPZ

Function

Is an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.

Alternative names

Myelin protein P0, Myelin peripheral protein, Myelin protein zero, MPP, MPZ

Recommended products

Rabbit Recombinant Monoclonal Myelin Protein Zero antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20383
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Myelin protein zero sometimes called MPZ or P0 is a structural protein in the peripheral nervous system. It has a molecular mass of approximately 30 kDa. Myelin protein zero is mostly found in Schwann cells where it plays an important role in the formation of the myelin sheath. This protein facilitates the adhesion between layers of the myelin membrane contributing to the compact structure of the myelin sheath surrounding nerve fibers.

Biological function summary

Several functions of nerve insulating layers involve myelin protein zero. It forms part of the myelin sheath critical for effective nerve signal transmission. The protein is associated with the Ig superfamily and interacts with other myelin components to maintain integrity and functionality. Myelin protein zero also affects the clustering of specific proteins and lipids within the myelin sheath making it an important component in maintaining the stability and function of peripheral nerves.

Pathways

Several important signal transmission processes involve the interactions of myelin protein zero. It is involved in the myelination pathway which is essential for insulating nerve fibers and speeding up action potential propagation. The protein is closely related to connexin 32 in this pathway another protein involved in maintaining the proper function of myelinated neurons. This coordination helps ensure efficient communication in the peripheral nervous system.

Associated diseases and disorders

Several neurological conditions link to abnormalities in myelin protein zero. Mutations in the gene encoding this protein can lead to Charcot-Marie-Tooth disease type 1B a hereditary neuropathy characterized by muscle weakness and sensory loss. Alterations in myelin protein zero are also associated with Dejerine-Sottas syndrome a severe neuropathy involving early-onset muscle weakness. The protein's relation to other myelin proteins like PMP22 often signifies its role in the molecular mechanisms underlying these demyelinating disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Blocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).
Negative control: liver, brain tissue (PMID 2578885, PMID 25288117).
All lanes: Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/5000 dilution
Lane 1: Mouse sciatic nerve lysate at 4 µg
Lane 2: Mouse liver lysate at 4 µg
Lane 3: Mouse brain lysate at 4 µg
Lane 4: Rat sciatic nerve lysate at 4 µg
Lane 5: Rat liver lysate at 4 µg
Lane 6: Rat brain lysate at 4 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 26-28 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunohistochemical analysis of paraffin-embedded human peripheral nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on Schwann cells of human peripheral nerves (PMID: 21057508).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Myelin Protein Zero was immunoprecipitated from Human Sciatic Nerve lysate with ab183868 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab183868 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Human Sciatic Nerve lysate 10ug (Input).
Lane 2: ab183868 IP in Human Sciatic Nerve lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183868 in Human Sciatic Nerve lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Predicted band size: 28 kDa
Exposure time: 3min
Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Membranous staining on Schwann cells of mouse sciatic nerves (PMID: 21057508).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunohistochemical analysis of paraffin-embedded mouse sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on Schwann cells of mouse sciatic nerves (PMID: 21057508).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Blocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature (PMID 10704451; PMID 1384988).
Negative control: liver tissue (PMID 2578885).
All lanes: Western blot - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868) at 1/1000 dilution
Lane 1: Human sciatic nerve lysate at 20 µg
Lane 2: Human fetal liver lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 28 kDa, 30 kDa, 54 kDa
Observed band size: 26-28 kDa, 33 kDa, 36 kDa, 40 kDa, 50 kDa
Exposure time: 30s
Immunohistochemistry (Frozen sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Membranous staining on Schwann cells of rat sciatic nerves (PMID: 21057508).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunohistochemical analysis of paraffin-embedded rat sciatic nerves labeling Myelin Protein Zero with ab183868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on Schwann cells of rat sciatic nerves (PMID: 21057508).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunocytochemistry/ Immunofluorescence analysis of S42 (rat sciatic nerve epithelial-like cell) labeling Myelin Protein Zero with ab183868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/200 was used as the counterstain antibody. Nuclear counterstain was DAPI (blue). Cells were fixed wiht 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.
Confocal image showing membranous and cytoplasmic staining in S42 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Anti-Myelin Protein Zero antibody [EPR20383] (ab183868)
Immunocytochemistry/ Immunofluorescence analysis of S16 (rat Sciatic nerve epithelial-like) labeling Myelin Protein Zero with ab183868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/200 was used as the counterstain antibody. Nuclear counterstain was DAPI (blue). Cells were fixed wiht 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.
Confocal image showing membranous and cytoplasmic staining in S16 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).