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AB229750

Anti-Myeloid leukemia factor 1 antibody

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(1 Publication)

Rabbit Polyclonal Myeloid leukemia factor 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLF1.

View Alternative Names

Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody (AB229750)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody (AB229750)

Paraffin-embedded human colon cancer tissue stained for Myeloid leukemia factor 1 using ab229750 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody (AB229750)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody (AB229750)

Paraffin-embedded human testis tissue stained for Myeloid leukemia factor 1 using ab229750 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MLF1.

P58340

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloid Leukemia Factor 1 (MLF1) sometimes referred to by the alternate name MLF1-MLL fusion protein is encoded by the MLF1 gene. It weighs approximately 36 kDa. This target functions mechanically as a nuclear protein involved in the regulation of gene expression. MLF1 predominantly expresses in hematopoietic tissues and influences the differentiation of myeloid progenitor cells by modulating transcriptional activity. It acts as an important player in cellular processes that govern cell cycle regulation.
Biological function summary

Binding activities associated with transcriptional regulation define Myeloid Leukemia Factor 1's critical functions. As part of the transcriptional machinery it modulates pathways that dictate hematopoietic lineage commitment and development. MLF1 does not operate distinctly but affects cellular processes that integrate into larger protein complexes. Through interactions with its associated protein partners MLF1 influences several downstream signaling pathways highlighting its role in hematopoiesis.

Pathways

The influence of MLF1 within hematopoietic signaling stands out. This protein plays an integral role in the hematopoietic cell lineages pathway and influences regulatory steps within apoptosis-related pathways. MLF1 interacts with several proteins involved in these pathways such as cyclin-dependent kinase subunits which are important for cell cycle regulation. This association ensures that MLF1 remains a strategic point within cellular signaling and decision-making processes related to cell differentiation and viability.

The aberrant expression or mutation of MLF1 has strong links with particular hematological malignancies such as acute myeloid leukemia (AML). AML features MLF1 interactions which can disrupt normal gene expression patterns contributing to oncogenesis. MLF1 has been closely associated with other dysregulated proteins in AML like the MLL (Mixed-Lineage Leukemia) fusion proteins which often result from chromosomal translocations. Additionally MLF1's role in certain bone marrow disorders highlights its relevance in the pathology of hematopoietic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
See full target information MLF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 56:102381 PubMed40199156

2025

Tumor-derived immunoglobulin-like transcript 3 inhibition reshapes the immunosuppressive tumor microenvironment and potentiates programmed cell death ligand 1 blockade immunotherapy in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Leirong Wang,Qing Li,Yanxin Sun,Shuyun Wang,Xuebing Fu,Xiufen Wang,Yan Zheng,Aiqin Gao,Yuping Sun,Juan Li
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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