AB90810

Anti-Myeloperoxidase antibody [2D4]

Name: Anti-Myeloperoxidase antibody [2D4] Mouse monoclonal (ab90810) | Abcam
Brand: Abcam Limited
SKU: AB90810
Price: 595 USD
Availability: InStock

(1 Review)

(39 Publications)

Mouse Monoclonal Myeloperoxidase antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 39 publications.

View Alternative Names

Myeloperoxidase, MPO

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (AB90810)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (AB90810)

ab90810 at 1/50 dilution staining Myeloperoxidase in rat white blood cells by Immunocytochemistry/ Immunofluorescence. Image shows green staining with ab90810 in neutrophils and monocytes.

Biotin

Biotin Anti-Myeloperoxidase antibody [2D4]
519 FITC

FITC Anti-Myeloperoxidase antibody [2D4]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D4

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab90810 is provided as a 0.2 µm filtered solution.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed41011190

2025

Nagilactone C from the Seeds of May Protect Against LPS-Induced Acute Lung Injury via STAT Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Chen,Jing Tang,Shijie Zhan,Yixian Qiu,Jing Li,Weiguang Shan,Youmin Ying

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e11873 PubMed40832885

2025

Succinate Dehydrogenase Subunit A (SDHA) Mediated Microglia Extracellular Traps Formation Participating in Cerebral Ischemic Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhao,Tao Li,Meijuan Dang,Ye Li,Jialiang Lu,Ziwei Lu,Zhiyang Chen,Qiao Huang,Yujie Chen,Yang Yang,Yuxuan Feng,Xiaoya Wang,Yating Jian,Heying Wang,Yingying Guo,Lei Zhang,Yu Jiang,Songhua Fan,Shengxi Wu,Hong Fan,Fang Kuang,Guilian Zhang

Journal of translational medicine 23:841 PubMed40713829

2025

Platelet membrane-coated nanoparticles inhibit platelet activation and neutrophil extracellular traps formation in acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Li,Zhaoxia Tang,Liangjian Kuang,Yongjian Wu,Xi Huang

Cell communication and signaling : CCS 23:181 PubMed40217343

2025

NETs activate the GAS6-AXL-NLRP3 axis in macrophages to drive morphine tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyan Tian,Haiyue Guo,Mengyao Zhang,Kunmao Jiang,Fan Hu,Yan Xu,Li Wan,Xiaokai Zhou,Yinbing Pan,Wentao Liu,Chun-Yi Jiang

Acta neuropathologica communications 13:33 PubMed39966968

2025

Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Song-I Seol,Sang-A Oh,Dashdulam Davaanyam,Ja-Kyeong Lee

Bioactive materials 47:181-197 PubMed39906647

2025

Targeting and reprogramming microglial phagocytosis of neutrophils by ginsenoside Rg1 nanovesicles promotes stroke recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Kaichao Hu,Junrui Ye,Pinglong Fan,Ruifang Zheng,Shasha Wang,Ye Peng,Yuan Ruan,Xu Yan,Zhao Zhang,Shifeng Chu,Naihong Chen

ACS nano 19:2944-2960 PubMed39772506

2025

Microemulsion-Inspired Polysaccharide Nanoparticles for an Advanced Targeted Thrombolytic Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Thibault de La Taille,Pierre Sarfati,Rachida Aid,Louise Fournier,Graciela Pavon-Djavid,Frédéric Chaubet,Cédric Chauvierre

Translational stroke research 16:1461-1473 PubMed39733198

2024

Neutrophil Extracellular Traps Induce Brain Edema Around Intracerebral Hematoma via ERK-Mediated Regulation of MMP9 and AQP4.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Tang,Jianhe Yue,Yihao Tao,Guanjian Zhao,Xiaoyao Yi,Maoxin Zhang,Ning Huang,Yuan Cheng

Experimental & molecular medicine 56:1717-1735 PubMed39085350

2024

Neutrophil extracellular traps mediate the crosstalk between plaque microenvironment and unstable carotid plaque formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Cao,Minghui Chen,Xinyu Jiao,Shuijie Li,Dong Wang,Yongxuan Zhan,Jiaju Li,Zhongfei Hao,Qingbin Li,Yang Liu,Yan Feng,Ruiyan Li,Hongjun Wang,Mingli Liu,Qiang Fu,Yongli Li

Scientific reports 14:16457 PubMed39014129

2024

Neutrophil extracellular traps promote erectile dysfunction in rats with diabetes mellitus by enhancing NLRP3-mediated pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xu,Yan Ren,Wenli Zou,Shuiyu Ji,Wei Shen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody [2D4]

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [2D4] (AB90810)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

Product promise