AB16886

Anti-Myeloperoxidase antibody [8F4]

Name: Anti-Myeloperoxidase antibody [8F4] (ab16886) | Abcam
Brand: Abcam Limited
SKU: AB16886
Price: 500 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal Myeloperoxidase antibody. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

Myeloperoxidase, MPO

1 Images

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [8F4] (AB16886)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [8F4] (AB16886)

Detection of MPO in Wehi3BD+ cells. Red, black and blue line represent the isotype control, cells only and ab16886 at 10 μg/ml, respectively.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8F4

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

0.2 µm filtered

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 10: PubMed34206992

2021

New Insights on NETosis Induced by : Dependence on ROS from Amoebas and Extracellular MPO Activity.

Applications

Unspecified application

Species

Unspecified reactive species

César Díaz-Godínez,Joshue Fabián Jorge-Rosas,Mario Néquiz,Santiago Martínez-Calvillo,Juan P Laclette,Carlos Rosales,Julio C Carrero

Oncogene 38:6461-6477 PubMed31332288

2019

Hepatoma-derived growth factor participates in Helicobacter Pylori-induced neutrophils recruitment, gastritis and gastric carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Huei Chu,Shih-Tsung Huang,Sheau-Fang Yang,Chia-Jung Li,Hung-Wei Lin,Bi-Chuang Weng,Shih-Ming Yang,Shih-Chung Huang,Jian-Ching Wu,Yi-Chen Chang,Zhi-Hong Wen,Yi-Ming Arthur Chen,Wen-Jeng Wu,Mei-Lang Kung,Po-Han Tai,Deng-Chyang Wu,Ming-Hong Tai

PloS one 14:e0214150 PubMed30889221

2019

Myeloperoxidase inhibition in mice alters atherosclerotic lesion composition.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel J Roth Flach,Chunyan Su,Eliza Bollinger,Christian Cortes,Andrew W Robertson,Alan C Opsahl,Timothy M Coskran,Kevin P Maresca,Edmund J Keliher,Phillip D Yates,Albert M Kim,Amit S Kalgutkar,Leonard Buckbinder

PloS one 6:e23915 PubMed21912650

2011

HIV-1 Tat-induced microgliosis and synaptic damage via interactions between peripheral and central myeloid cells.

Applications

IHC-FoFr

Species

Mouse

Shao-Ming Lu,Marie-Ève Tremblay,Irah L King,Jin Qi,Holly M Reynolds,Daniel F Marker,John J P Varrone,Ania K Majewska,Stephen Dewhurst,Harris A Gelbard

Laboratory investigation; a journal of technical m 88:1192-203 PubMed18794850

2008

Activation of hepatic stellate cells is associated with cytokine expression in thioacetamide-induced hepatic fibrosis in mice.

Applications

IHC-Fr

Species

Mouse

Rebeca Salguero Palacios,Martin Roderfeld,Stefanie Hemmann,Timo Rath,Srebrena Atanasova,Annette Tschuschner,Olav A Gressner,Ralf Weiskirchen,Jürgen Graf,Elke Roeb

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody [8F4]

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [8F4] (AB16886)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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