Rabbit Polyclonal Myeloperoxidase antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 15 publications.
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- International journal of molecular sciences 24:2023Early Depletion of Neutrophils Reduces Retinal Inflammation and Neovascularization in Mice with Oxygen-Induced Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDevy Deliyanti,Varaporn Suphapimol,Phoebe Ang,Xiuying Tang,Abhirup Jayasimhan,Jennifer L Wilkinson-BerkaPubMed 37958664
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e226742022Lymphatic endothelial cell RXRα is critical for 9-cis-retinoic acid-mediated lymphangiogenesis and prevention of secondary lymphedema.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCynthia Sung,Wan Jiao,Sun Young Park,Michael Cooper,Antoun Bouz,Dahae Choi,Eunson Jung,Gene Kim,Young Kwon Hong,Alex K WongPubMed 36520015
- Frontiers in physiology 13:9475372022NF-κB-dependent repression of Sox18 transcription factor requires the epigenetic regulators histone deacetylases 1 and 2 in acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvgeny A Zemskov,Christine M Gross,Saurabh Aggarwal,Marina A Zemskova,Xiaomin Wu,Chenxin Gu,Ting Wang,Haiyang Tang,Stephen M BlackPubMed 35991176
- Journal of biophotonics 15:e2022000732022Label-free analysis of inflammatory tissue remodeling in murine lung tissue based on multiphoton microscopy, Raman spectroscopy and machine learning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucas Kreiss,Ingo Ganzleben,Alexander Mühlberg,Paul Ritter,Dominik Schneidereit,Christoph Becker,Markus F Neurath,Oliver Friedrich,Sebastian Schürmann,Maximilian WaldnerPubMed 35611635
- Nature 602:496-5022022Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanyan Deng,Yang Bai,Fan Deng,Youdong Pan,Shenglin Mei,Zengzhang Zheng,Rui Min,Zeyu Wu,Wu Li,Rui Miao,Zhibin Zhang,Thomas S Kupper,Judy Lieberman,Xing LiuPubMed 35110732
- Biomolecules 11:2021Role of Inflammation and Redox Status on Doxorubicin-Induced Cardiotoxicity in Infant and Adult CD-1 Male Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Reis-Mendes,Ana Isabel Padrão,José Alberto Duarte,Salomé Gonçalves-Monteiro,Margarida Duarte-Araújo,Fernando Remião,Félix Carvalho,Emília Sousa,Maria Lourdes Bastos,Vera Marisa CostaPubMed 34827723
- Pharmaceuticals (Basel, Switzerland) 14:2021Inflammation as a Possible Trigger for Mitoxantrone-Induced Cardiotoxicity: An In Vivo Study in Adult and Infant Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Reis-Mendes,José Luís Dores-Sousa,Ana Isabel Padrão,Margarida Duarte-Araújo,José Alberto Duarte,Vítor Seabra,Salomé Gonçalves-Monteiro,Fernando Remião,Félix Carvalho,Emília Sousa,Maria Lourdes Bastos,Vera Marisa CostaPubMed 34073506
- Theranostics 11:4567-45842021Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZih-Chan Lin,Tsong-Long Hwang,Tse-Hung Huang,Kohei Tahara,Jiří Trousil,Jia-You FangPubMed 33754014
- Bioscience reports 40:2020CTRP3 ameliorates cerulein-induced severe acute pancreatitis in mice via SIRT1/NF-κB/p53 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyang Lv,Yuan He,Mingli Wei,Guiyun Xu,Chuang Chen,Zhen Xu,Zhilong DingPubMed 32219332
- Infection and immunity 87:2019Tissue Tropism in Streptococcal Infection: Wild-Type M1T1 Group A Is Efficiently Cleared by Neutrophils Using an NADPH Oxidase-Dependent Mechanism in the Lung but Not in the Skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenfang Lei et. al.PubMed 31331954
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:11593004). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low density lipoprotein particles and limits vascular damage (By similarity).
Alternative names
Myeloperoxidase, MPO, Mpo
Recommended products
Rabbit Polyclonal Myeloperoxidase antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab208670.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab139748)
IHC image of Myeloperoxidase staining in Mouse normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139748, 1μg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Myeloperoxidase antibody (ab139748)
The band observed at 64 kDa could potentially be a cleaved form of Myeloperoxidase due to the presence of both a 15 amino acid signal peptide and a 123 amino acid propeptide.
The band observed at 48 kDa could also represent the Myeloperoxidase heavy chain.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-Myeloperoxidase antibody (ab139748) at 1 µg/mL
Lane 1: Mouse Bone Marrow Tissue Lysate at 10 µg
Lane 2: Spleen (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 48 kDa, 63 kDa
Exposure time: 30s
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