AB210563

Anti-Myeloperoxidase antibody

Name: Anti-Myeloperoxidase antibody (ab210563) | Abcam
Brand: Abcam Limited
SKU: AB210563
Price: 510 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal Myeloperoxidase antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MPO aa 400 to C-terminus.

View Alternative Names

Myeloperoxidase, MPO

7 Images

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

Immunohistochemical analysis of whole-mount 2-day post-fertilization zebrafish embryo, labeling Myeloperoxidase using ab210563 at a 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

Immunohistochemical analysis of paraformaldehyde-fixed, whole-mount zebrafish embryos labeling Myeloperoxidase using ab210563 at a 1/200 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

ab210563 detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.

Sample : Paraformaldehyde-fixed 3 days-post-fertilization zebrafish embryo.

Green : Mpx stained by ab210563 at a 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

ab210563 detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.

Sample : Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.

Green : Mpx stained by ab210563 at a 1/100 dilution.

Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

ab210563 detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.

Sample : Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.

Green : Mpx stained by ab210563 at a 1/100 dilution.

Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

ab210563 detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.

Sample : Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.

Green : Mpx stained by ab210563 at a 1/100 dilution.

Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

ab210563 detects Mpx protein on whole mount zebrafish by immunohistochemical analysis.

Sample : Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.

Green : Mpx stained by ab210563 at a 1/100 dilution.

Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Human MPO aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7199 PubMed40789835

2025

A continuously oxygenated macroencapsulation system enables high-density packing and delivery of insulin-secreting cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tung T Pham,Phuong L Tran,Linda A Tempelman,Simon G Stone,Christopher Piccirillo,Alan Li,James A Flanders,Minglin Ma

Nature communications 15:6774 PubMed39117721

2024

In situ assembly of an injectable cardiac stimulator.

Applications

Unspecified application

Species

Unspecified reactive species

Umut Aydemir,Abdelrazek H Mousa,Cedric Dicko,Xenofon Strakosas,Muhammad Anwar Shameem,Karin Hellman,Amit Singh Yadav,Peter Ekström,Damien Hughes,Fredrik Ek,Magnus Berggren,Anders Arner,Martin Hjort,Roger Olsson

Frontiers in physiology 15:1338858 PubMed38410809

2024

Salinity change evokes stress and immune responses in Atlantic salmon with microalgae showing limited potential for dietary mitigation.

Applications

Unspecified application

Species

Unspecified reactive species

Doret R van Muilekom,Jonas Mueller,Jacqueline Lindemeyer,Thekla Schultheiß,Edmund Maser,Henrike Seibel,Alexander Rebl,Carsten Schulz,Tom Goldammer

Frontiers in pharmacology 13:832928 PubMed35359845

2022

Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Gence,Danielle Fernezelian,Matthieu Bringart,Bryan Veeren,Armelle Christophe,François Brion,Olivier Meilhac,Jean-Loup Bascands,Nicolas Diotel

International journal of experimental pathology 103:13-22 PubMed34725870

2021

Soy diet induces intestinal inflammation in adult Zebrafish: Role of OTX and P53 family.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Micheloni,Marta Carnovali,Giorgia Millefanti,Manuel Rizzetto,Vittoria Moretti,Giuseppe Montalbano,Francesco Acquati,Cristina Giaroni,Roberto Valli,Lucy Costantino,Fulvio Ferrara,Giuseppe Banfi,Massimo Mariotti,Giovanni Porta

Biomedicines 9: PubMed33917641

2021

Soybean Meal-Dependent Intestinal Inflammation Induces Different Patterns of Bone-Loss in Adult Zebrafish Scale.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Carnovali,Roberto Valli,Giuseppe Banfi,Giovanni Porta,Massimo Mariotti

American journal of physiology. Gastrointestinal and liver physiology 319:G212-G226 PubMed32597709

2020

Broad-spectrum antibiotics alter the microbiome, increase intestinal , and decrease hepatic steatosis in zebrafish short bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn M Maselli,Kristin Gee,Mubina Isani,Alexa Fode,Kathy A Schall,Tracy C Grikscheit

The Journal of surgical research 255:86-95 PubMed32543383

2020

Remnant Intestinal Length Defines Intestinal Adaptation and Hepatic Steatosis: Two Zebrafish Models.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn M Maselli,Kristin Gee,Tracy C Grikscheit

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

IHC - Wholemount - Anti-Myeloperoxidase antibody (AB210563)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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