Rabbit Polyclonal Myeloperoxidase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 78 publications.
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- Journal of neurogastroenterology and motility 29:486-5002023Imbalance of Innate and Adaptive Immunity in Esophageal Achalasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Yao,Zuqiang Liu,Weifeng Chen,Jiaqi Xu,Xiaoyue Xu,Jiaxin Xu,Liyun Ma,Xiaoqing Li,Quanlin Li,Pinghong ZhouPubMed 37586778
- BMC gastroenterology 23:2372023Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric patients undergoing parental liver transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaling Liu,Huigang Shu,Ping Wan,Xiaodong Wang,Hong XiePubMed 37442949
- International journal of molecular sciences 24:2023NETs Promote Inflammatory Injury by Activating Pathway in Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhao,Ningxin Zhen,Qichao Zhou,Jian Lou,Wei Cui,Gensheng Zhang,Baoping TianPubMed 36982193
- Frontiers in immunology 14:11015692023Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLena Lavie,Erez Si-On,Aaron HoffmanPubMed 36911715
- Journal of neuroinflammation 18:2332021Inhibition of vascular adhesion protein 1 protects dopamine neurons from the effects of acute inflammation and restores habit learning in the striatum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSerena Becchi,Alberto Buson,Bernard W BalleinePubMed 34654450
- Frontiers in pharmacology 12:7386892021L. Extracts Promote Activity of FXR to Ameliorate Alcoholic Liver Disease: Targeting Liver Lipid Deposition and Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Yu Cui,Xin Han,Yu-Chen Jiang,Jia-Yi Dou,Kun-Chen Yao,Zhong-He Hu,Ming-Hui Yuan,Xiao-Xue Bao,Mei-Jie Zhou,Yue Liu,Li-Hua Lian,Xian Zhang,Ji-Xing Nan,Yan-Ling WuPubMed 34690775
- Stem cell research & therapy 12:4292021Addressing the liver progenitor cell response and hepatic oxidative stress in experimental non-alcoholic fatty liver disease/non-alcoholic steatohepatitis using amniotic epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMihiri Goonetilleke,Nathan Kuk,Jeanne Correia,Alex Hodge,Gregory Moore,Michael P Gantier,George Yeoh,William Sievert,Rebecca LimPubMed 34321089
- The Journal of experimental medicine 218:2021Impaired respiratory burst contributes to infections in PKCδ-deficient patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna-Lena Neehus,Kunihiko Moriya,Alejandro Nieto-Patlán,Tom Le Voyer,Romain Lévy,Ahmet Özen,Elif Karakoc-Aydiner,Safa Baris,Alisan Yildiran,Engin Altundag,Manon Roynard,Kathrin Haake,Mélanie Migaud,Karim Dorgham,Guy Gorochov,Laurent Abel,Nico Lachmann,Figen Dogu,Sule Haskologlu,Erdal İnce,Jamel El-Benna,Gulbu Uzel,Ayca Kiykim,Kaan Boztug,Marion R Roderick,Mohammad Shahrooei,Paul A Brogan,Hassan Abolhassani,Gonca Hancioglu,Nima Parvaneh,Alexandre Belot,Aydan Ikinciogullari,Jean-Laurent Casanova,Anne Puel,Jacinta BustamantePubMed 34264265
- Asian Pacific journal of cancer prevention : APJCP 22:2143-21522021Isolated Myeloperoxidase Immunohistochemical Expression in Bone Marrow Biopsy Depicts Clinical Outcomes in Adults with Typical B-Acute Lymphoblastic Leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaha Mohamed Fawzy,Amal Abd El-hafez,Shaimaa El-Ashwah,Tarek E. Abouzeid,Maryan Fahmi Waheeb,Maha Saif,Metwaly Ibrahim Mortada,Mayada A. Ghannam,Heba ShetaPubMed 34319037
- Journal of cellular and molecular medicine 25:6511-65232021MiR-144-induced KLF2 inhibition and NF-kappaB/CXCR1 activation promote neutrophil extracellular trap-induced transfusion-related acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiping Le,Yize Wu,Wei Liu,Chenggao Wu,Piaoping Hu,Juan Zou,Linju KuangPubMed 34120407
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Common marmoset | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Common marmoset | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Common marmoset | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Common marmoset | Dilution info - | Notes - |
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Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Alternative names
Myeloperoxidase, MPO
Recommended products
Rabbit Polyclonal Myeloperoxidase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 78 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
ab45977 has been batch tested using human lysates/tissues only. Some customers have successfully used ab45977 with rat. Please contact Abcam Scientific Support for more information.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
This image is courtesy of a customer review submitted by Antibody Solutions Ltd.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab45977)
ab45977 (1/50) staining Myeloperoxidase in paraffin-embbeded human spleen tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.
Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody (ab45977)
ab45977 stained in HL60 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45977 at 5μg/ml and ab72791 (Mouse monoclonal to alpha Tubulin - Loading Control) at 1ug/ml overnight at +4°C. The secondary antibodies used were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 used at 1 ug/ml (colored green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) ab150116 (pseudo-colored red) used at 2ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43μM for 1hour at room temperature.
Western blot - Anti-Myeloperoxidase antibody (ab45977)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45977 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406
All lanes: Western blot - Anti-Myeloperoxidase antibody (ab45977) at 1 µg/mL
All lanes: HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 84 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab45977)
IHC image of 45977 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45977, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-Myeloperoxidase antibody (ab45977)
The mature myeloperoxidase protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). Our immunogen sequence is within the myeloperoxidase light chain. In HL60 cells, ab45977 detects bands at approximately 84-kDa, corresponding to the expected MW of full-length Myeloperoxidase protein, and at 12-kDa, corresponding to the expected MW of myeloperoxidase light chain.
All lanes: Western blot - Anti-Myeloperoxidase antibody (ab45977) at 1 µg/mL
All lanes: HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Predicted band size: 83 kDa
Observed band size: 12 kDa, 35 kDa, 75 kDa, 84 kDa
Exposure time: 3min
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