• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB65871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Myeloperoxidase using ab65871.

Myeloperoxidase was detected in paraffin-embedded section of human colon tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB65871)

This image shows human ovary tissue stained with ab65871 at 1µg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB65871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Myeloperoxidase using ab65871.

Myeloperoxidase was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65871 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB65871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Myeloperoxidase using ab65871.

Myeloperoxidase was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab65871 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB65871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Myeloperoxidase using ab65871.

Myeloperoxidase was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab65871 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB65871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Myeloperoxidase using ab65871.

Myeloperoxidase was detected in paraffin-embedded section of rat colon tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab65871 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• WB

Unknown

Western blot - Anti-Myeloperoxidase antibody (AB65871)

All lanes:

Western blot - Anti-Myeloperoxidase antibody (ab65871) at 2 µg/mL

All lanes:

rat brain tissue lysate

Predicted band size: 83 kDa

Observed band size: 170 kDa

false

• WB

CiteAb

Western blot - Anti-Myeloperoxidase antibody (AB65871)

Myeloperoxidase western blot using anti-Myeloperoxidase antibody ab65871. Publication image and figure legend from Horinokita, I., Hayashi, H., et al., 2019, Int J Mol Sci, PubMed 31640144.

ab65871 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab65871 please see the product overview.

(A) Changes in tissue elastase activity in the vehicle-treated (-) sham and ME groups and sivelestat-treated (+) sham and ME groups on day 1 after surgery. * Significant difference from the vehicle-treated sham group (p < 0.05).

Significant difference from the vehicle-treated ME group (p < 0.05). Each value represents the mean ± SD of 6

animals; (B) Levels of myeloperoxidase (MPO) protein in the vehicle-treated (-) sham and ME groups and sivelestat-treated (+) sham and ME groups on day 1 after surgery. Bands corresponding to MPO were scanned, and the scanned band was normalized by β-actin on the same blot. The results are expressed as the mean ratio of the non-operated (control) group ± SD (n = 8 each). * Significant difference from the sham group (p < 0.05); (C) Images of double staining (merge, c, f, i, and l) with Hoechst 33342 (blue, a, d, g, and j) and MPO (green, b, e, h, and k) for the vehicle-treated sham and ME groups and sivelestat-treated sham and ME groups on day 1 after surgery. The scale bar represents 100 μm; (D) The number of MPO-positive cells in the vehicle-treated (-) sham and ME groups and sivelestat-treated (+) sham and ME groups on day 1 after surgery was counted. Five sections were made per animal, and 266–722 cells were counted per section, and the average of 5 sections per animal was calculated. The values for MPO-positive cells are presented as the mean ± SD (n = 5 each). * Significant difference from the sham group (p < 0.05).

false