Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody detecting Myeloperoxidase in IHC-P. Suitable for Human.
- Over 320 publications
- Trusted since 2002
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- Biology of sex differences 14:852023Sex-based differences in natural killer T cell-mediated protection against diet-induced steatohepatitis in Balb/c mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Cuño-Gómiz,Estefanía de Gregorio,Anna Tutusaus,Patricia Rider,Nuria Andrés-Sánchez,Anna Colell,Albert Morales,Montserrat MaríPubMed 37964320
- Stem cell research & therapy 14:3252023Systemically delivered adipose stromal vascular fraction mitigates radiation-induced gastrointestinal syndrome by immunomodulating the inflammatory response through a CD11b cell-dependent mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLydia Bensemmane,Fabien Milliat,Xavier Treton,Christine LinardPubMed 37953266
- JVS-vascular science 4:1001262023Tissue factor targeting peptide enhances nanoparticle binding and delivery of a synthetic specialized pro-resolving lipid mediator to injured arteries.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth S Levy,Alexander S Kim,Evan Werlin,Mian Chen,Brian E Sansbury,Matthew Spite,Tejal A Desai,Michael S ContePubMed 38045567
- Scientific reports 13:141092023Chronodisruption of the acute inflammatory response by night lighting in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViera Jerigova,Michal Zeman,Monika OkuliarovaPubMed 37644084
- Nature 619:585-5942023An atlas of healthy and injured cell states and niches in the human kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlue B Lake,Rajasree Menon,Seth Winfree,Qiwen Hu,Ricardo Melo Ferreira,Kian Kalhor,Daria Barwinska,Edgar A Otto,Michael Ferkowicz,Dinh Diep,Nongluk Plongthongkum,Amanda Knoten,Sarah Urata,Laura H Mariani,Abhijit S Naik,Sean Eddy,Bo Zhang,Yan Wu,Diane Salamon,James C Williams,Xin Wang,Karol S Balderrama,Paul J Hoover,Evan Murray,Jamie L Marshall,Teia Noel,Anitha Vijayan,Austin Hartman,Fei Chen,Sushrut S Waikar,Sylvia E Rosas,Francis P Wilson,Paul M Palevsky,Krzysztof Kiryluk,John R Sedor,Robert D Toto,Chirag R Parikh,Eric H Kim,Rahul Satija,Anna Greka,Evan Z Macosko,Peter V Kharchenko,Joseph P Gaut,Jeffrey B Hodgin,Michael T Eadon,Pierre C Dagher,Tarek M El-Achkar,Kun Zhang,Matthias Kretzler,Sanjay JainPubMed 37468583
- Frontiers in neuroscience 17:11572362023Protection against brain injury after ischemic stroke by intravenous human amnion epithelial cells in combination with tissue plasminogen activator.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiz J Barreto-Arce,Hyun Ah Kim,Siow Teng Chan,Rebecca Lim,Grant R Drummond,Henry Ma,Thanh G Phan,Christopher G Sobey,Shenpeng R ZhangPubMed 37397458
- Aging and disease 14:966-9912023Lymphocyte-Related Immunomodulatory Therapy with Siponimod (BAF-312) Improves Outcomes in Mice with Acute Intracerebral Hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiying Zhang,Yinuo Li,Juyuan Shi,Li Zhu,Yinming Dai,Peiji Fu,Simon Liu,Michael Hong,Jiewen Zhang,Jian Wang,Chao JiangPubMed 37191423
- Veterinary dermatology 34:441-4512023Clinical and histopathological features of rostrolateral nasal alar arteriopathy of German shepherd dogs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDrew A Fleischman,Elizabeth A Mauldin,Andrew Lowe,Christine L Cain,Charles W BradleyPubMed 37221296
- BMC women's health 23:2292023Association between tissue stress reaction and ACE2/TMPRSS2 expression in endometria of reproductive aged women before and during Covid-19 pandemic.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanae Ogawa,Khaleque N Khan,Akemi Koshiba,Akira Fujishita,Go Horiguchi,Satoshi Teramukai,Kyoko Itoh,Sun-Wei Guo,Taisuke MoriPubMed 37142998
- Disease markers 2022:58514122022Tanshinone IIA Improves Acute Gouty Arthritis in Rats through Regulating Neutrophil Activation and the NLRP3 Inflammasome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianjie Xu,Xiao Liu,Yurong Zhang,Tao Jia,Limei Li,Yan Du,Wenhui Chen,Shan ZhangPubMed 36578443
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human MPO. Database link P05164
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Reactivity data
Select an application| ICC | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Alternative names
Myeloperoxidase, MPO
Recommended products
Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody detecting Myeloperoxidase in IHC-P. Suitable for Human.
- Over 320 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human MPO. Database link P05164
- Purification technique
- Affinity purification Protein A
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody and is validated for use in ICC, IHC-P in human, mouse samples.
Anti-Myeloperoxidase antibody (Anti-MPO antibody) (ab9535) has been cited over 320 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Myeloperoxidase antibody (ab9535) has high sensitivity and specificity.
Anti-Myeloperoxidase antibody (ab9535) has 36 independent reviews from customers.
Anti-Myeloperoxidase antibody (ab9535) specifically detects Myeloperoxidase (UniProt ID: P05164; Molecular weight: 79kDa) and is sold in 500 µL selling sizes.
Myeloperoxidase (MPO) is an enzyme predominantly found in neutrophils, where it plays a critical role in the immune response by producing hypochlorous acid to kill pathogens. However, MPO can also contribute to tissue damage and inflammation, and its deficiency is linked to increased susceptibility to infections, particularly fungal infections like those caused by Candida species. MPOcontributes to inflammation by producing reactive oxygen species (ROS) and hypochlorous acid (HOCl) during the immune response. This enzyme's activity is linked to various inflammatory diseases, including atherosclerosis and neuroinflammation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Image courtesy of Carl Hobbs, Kings College London, U.KImmunocytochemistry - Anti-Myeloperoxidase antibody (ab9535)
ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab9535)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human B cell lymphoma tissue, staining Myeloperoxidase with ab9535.
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