AB9535

Anti-Myeloperoxidase antibody

Name: Myeloperoxidase (MPO) antibody, rabbit polyclonal (ab9535) | Abcam
Brand: Abcam Limited
SKU: AB9535
Price: 450 USD
Availability: InStock
Rating: 4 (37 reviews)

(37 Reviews)

(415 Publications)

Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody detecting Myeloperoxidase in IHC-P. Suitable for Human.

- Over 320 publications
- Trusted since 2002

View Alternative Names

Myeloperoxidase, MPO

3 Images

AbReview36632****

Immunocytochemistry - Anti-Myeloperoxidase antibody (AB9535)

ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.

Image courtesy of Carl Hobbs, Kings College London, U.K

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB9535)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB9535)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human B cell lymphoma tissue, staining Myeloperoxidase with ab9535.

CiteAb

Immunohistochemistry - Anti-Myeloperoxidase antibody (AB9535)

Myeloperoxidase immunohistochemistry using Anti-Myeloperoxidase antibody ab9535. Publication image and figure legend from Rymaszewski, A. L., Tate, E., et al., 2014, Cancers (Basel), Pubmed 24821130.

Genetic deletion of MPO slows tumor growth in a heterotopic syngeneic tumor model. WT and MPO-/- C57BL/6J mice were injected in the right flank with Lewis Lung Carcinoma cells. (A) Tumor volume was monitored over the course of 17 days. MPO-/- mice exhibited smaller tumors compared to WT. Wild type mice treated with KYC were no different than PBS treatment; (B) Tumors were surgically resected and weighed on day 17. The weight of tumors from MPO-/- mice was significantly smaller compared to WT mice; (C) Representative images of tumors stained for Ly6G, MPO and apoptosis marker cleaved caspase-3 (4x); (D) Representative images of non-necrotic sections were immunostained for the neutrophil marker Ly6G, MPO, cleaved caspase-3 and proliferation marker Ki-67 (40x); (E) Tumor infiltrating neutrophils were elevated in MPO-/- mice compared to WT and KYC treated mice.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human MPO.

P05164

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody and is validated for use in ICC, IHC-P in human, mouse samples.

Anti-Myeloperoxidase antibody (Anti-MPO antibody) (ab9535) has been cited over 320 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Myeloperoxidase antibody (ab9535) has high sensitivity and specificity.

Anti-Myeloperoxidase antibody (ab9535) has 36 independent reviews from customers.

Anti-Myeloperoxidase antibody (ab9535) specifically detects Myeloperoxidase (UniProt ID: P05164; Molecular weight: 79kDa) and is sold in 500 µL selling sizes.

Myeloperoxidase (MPO) is an enzyme predominantly found in neutrophils, where it plays a critical role in the immune response by producing hypochlorous acid to kill pathogens. However, MPO can also contribute to tissue damage and inflammation, and its deficiency is linked to increased susceptibility to infections, particularly fungal infections like those caused by Candida species. MPOcontributes to inflammation by producing reactive oxygen species (ROS) and hypochlorous acid (HOCl) during the immune response. This enzyme's activity is linked to various inflammatory diseases, including atherosclerosis and neuroinflammation.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

style

left-column

Publications (415)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40927979

2025

Pharmacological inhibition of casein kinase II attenuates metaflammation in a murine model of diet‑induced metabolic dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Porchietto,Eleonora Aimaretti,Giacomo Einaudi,Gustavo Ferreira Alves,Debora Collotta,Enrica Marzani,Leonardo Camillò,Chiara Rubeo,Raffaella Mastrocola,Natasha Irrera,Manuela Aragno,Carlo Cifani,Massimo Collino

Cancers 17: PubMed40805151

2025

Evaluating Therapeutic Efficacy of Intravesical Xenogeneic Urothelial Cell Treatment Alone and in Combination with Chemotherapy or Immune Checkpoint Inhibition in a Mouse Non-Muscle-Invasive Bladder Cancer Model.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Rong Shyr,Ching-Feng Wu,Kai-Cheng Yang,Wen-Lung Ma,Chi-Ping Huang

Journal of inflammation (London, England) 22:33 PubMed40797324

2025

Hydroxychloroquine reduces metastatic tumor burden in pancreatic adenocarcinoma through myeloperoxidase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Britney Niemann,Pavan Rao,Quinn Hopen,Kaitlyn Landreth,Angisha Basnet,Werner Geldenhuys,Tracy W Liu,Brian A Boone

Science advances 11:eady8566 PubMed40779638

2025

Heparan sulfate fine-tuned interleukin-1 (IL-1) signaling inhibits insulin secretion of grafted pancreatic islets.

Applications

Unspecified application

Species

Unspecified reactive species

Selina Wrublewsky,Sandra Rother,Franziska Pohlemann,Toni Radanovic,Fabian Junker,Anne S Boewe,Stefan Schunk,Leticia P Roma,Gloria Ruiz-Gómez,M Teresa Pisabarro,Patrick E MacDonald,Michael D Menger,Matthias W Laschke,Emmanuel Ampofo

Nature communications 16:6807 PubMed40707439

2025

Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qingzhou Chen,Pengxiao Sun,Jiaxin Zhou,Tantan Long,An Xiao,Zhuoliang Liu,Shihui Xu,Wenjing Lei,Rui Zhang,Jianwei Tian,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Jing Nie

Translational stroke research 16:2026-2034 PubMed40670870

2025

Modified Intracranial Aneurysm Rupture Rat Model with Angiographic Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

William Wei-Lin Pan,Masahiko Itani,Kostadin Karagiozov,Teppei Komatsu,Hiroki Ohta,Hirokazu Koseki,Shunsuke Hataoka,Yoshiki Arakawa,Hirotaka James Okano,Tomohiro Aoki,Yuichi Murayama

Liver research (Beijing, China) 9:157-168 PubMed40620498

2025

Rhizoma Atractylodis Macrocephalae reduces HFD-induced MAFLD in mice through activated AMPK-mediated inhibition of fatty acid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zheng,Ruishuo Zhang,Yijing Xin,Yuge Zhou,Jiacheng Lin,Weifan Huang,Fang Wang,Liu Yang,Xuehua Sun,Xiaoni Kong

Cells 14: PubMed40498004

2025

Prognostic Significance of Macrophage Phenotypes in Peri-Tumoral Normal Tissue of Early-Stage Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Marcel Hirschmann,Sören Schnellhardt,Matthias Rübner,Sarah Segelhorst,Oliver Ott,Ramona Erber,Christoph Daniel,Maike Büttner-Herold,Paul Gass,Rainer Fietkau,Luitpold Distel

Anatomy & cell biology 58:483-488 PubMed40405544

2025

A potential interference of chemoreception in inflamed vomeronasal organ with experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungsook Jung,Deokho Ko,Sungmoo Hong,Irene Ortiz-Leal,Pablo Sanchez-Quinteiro,Meejung Ahn,Changjong Moon,Taeyoung Kang,Taekyun Shin,Jeongtae Kim

Frontiers in immunology 16:1528471 PubMed40313936

2025

CD19 B cell depletion: a novel strategy to alleviate ischemic stroke damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xu,Jing Peng,Yizhong Yan,Min Gao,HongJing Zang,Lamei Cheng,Yu Zhou

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody

Immunocytochemistry - Anti-Myeloperoxidase antibody (AB9535)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (AB9535)

Immunohistochemistry - Anti-Myeloperoxidase antibody (AB9535)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (415)

Product promise