Rabbit Monoclonal Myeloperoxidase antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95017.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57464.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134657.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022)")
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--flow-cytometry-intracellular-img126550.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022)")
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--flow-cytometry-intracellular-img421281.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (AB236022)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Alternative names
Myeloperoxidase, MPO
Recommended products
Rabbit Monoclonal Myeloperoxidase antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17996
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific to Myeloperoxidase light chain.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236022 is the carrier-free version of Anti-Myeloperoxidase antibody [EPR17996] ab188211.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95017.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR17996] ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of human spleen [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Myeloperoxidase antibody [EPR17996] ab188211).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57464.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR17996] ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of mouse spleen [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Myeloperoxidase antibody [EPR17996] ab188211).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134657.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR17996] ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of rat spleen [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Myeloperoxidase antibody [EPR17996] ab188211).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--flow-cytometry-intracellular-img126550.jpg "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)")
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed mouse PBMC labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR17996] ab188211 at 1/500 dilution (Right) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; Left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Mouse peripheral blood mononuclear cells stained intracellularly with Anti-Myeloperoxidase antibody [EPR17996] ab188211 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Myeloperoxidase antibody [EPR17996] ab188211).
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr17996-bsa-and-azide-free-ab236022--flow-cytometry-intracellular-img421281.jpg "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)")
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] - BSA and Azide free (ab236022)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Myeloperoxidase antibody [EPR17996] ab188211).
Flow cytometry staining of C57 BL/6 mouse bone marrow cells with Anti-Myeloperoxidase antibody [EPR17996] ab188211 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30min at 22°C in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-Myeloperoxidase antibody [EPR17996] ab188211 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 0.2 μg/ml (1/10200)) for 30min at 22°C. The cells were simultaneously stained with Ly6G.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com