Anti-Myeloperoxidase antibody [EPR20257]
- RabMAb
- Recombinant
- 20ul selling size
- What is this?
5
(7 Reviews)
|
(350 Publications)
Anti-Myeloperoxidase antibody [EPR20257] (ab208670) is a rabbit monoclonal antibody detecting Myeloperoxidase in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 250 publications
Rat version of this clone also available as ab300650. Same clone, same staining pattern, different species antibody backbone.
Learn more about chimeric antibodies
View Alternative Names
Myeloperoxidase, MPO
- WB
Supplier Data
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Blocking/Dilution buffer : 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID : 3029127 PMID : 2154223.
Negative control : HeLa PMID 12040446.
All lanes:
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution
Lane 1:
Human fetal spleen lysate at 20 µg
Lane 2:
Rat spleen lysate at 20 µg
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 59 kDa
false
Exposure time: 10s
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HL-60 cell line.
Negative control : HeLa (PMID : 12040446).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human spleen is observed [PMID : 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa cells (left panel) and HL-60cells (right panel) labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and unlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control : HeLa (PMID : 12040446).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID : 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730; left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID : 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of rat spleen is observed [PMID : 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Blocking/Dilution buffer : 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID : 2154223 PMID : 8384653.
All lanes:
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution
All lanes:
HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 39 kDa,59 kDa,89 kDa
false
Exposure time: 1s
- WB
Supplier Data
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)
Blocking/Dilution buffer : 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID : 3029127 PMID : 2154223.
All lanes:
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution
All lanes:
Mouse spleen lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 59 kDa
false
Exposure time: 10s
Related conjugates and formulations (4)
-
Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Myeloperoxidase antibody [EPR20257]
-
Biotin Anti-Myeloperoxidase antibody [EPR20257]
-
HRP Anti-Myeloperoxidase antibody [EPR20257]
Reactivity data
Product details
Anti-Myeloperoxidase antibody [EPR20257] (ab208670) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Myeloperoxidase antibody [EPR20257] (ab208670) was first used in a scientific publication in 2018 and has been cited over 162 times in peer reviewed journals. It's performance in IHC and Western Blot in mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Myeloperoxidase antibody [EPR20257] (ab208670) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Myeloperoxidase antibody [EPR20257] (ab208670) has 5 independent reviews from customers.
Anti-Myeloperoxidase antibody [EPR20257] (ab208670) specifically detects Myeloperoxidase (UniProt ID: P05164; Molecular weight: 79kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR20257 - ab221847.
Antibody clone EPR20257 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Biotin (ab225474, ab322301).
- Recombinant format for unrivalled batch-batch consistency: no need for same-lot requests
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (350)
Recent publications for all applications. Explore the full list and refine your search
Journal of translational medicine 23:1074 PubMed41074088
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 16:8870 PubMed41053178
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell communication and signaling : CCS 23:416 PubMed41039446
2025
Applications
Unspecified application
Species
Unspecified reactive species
iScience 28:113519 PubMed41035689
2025
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of clinical investigation 135: PubMed41031886
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of nanobiotechnology 23:612 PubMed41023976
2025
Applications
Unspecified application
Species
Unspecified reactive species
Molecular medicine (Cambridge, Mass.) 31:304 PubMed41023796
2025
Applications
Unspecified application
Species
Unspecified reactive species
Stem cell research & therapy 16:507 PubMed41013769
2025
Applications
Unspecified application
Species
Unspecified reactive species
Histology and histopathology :18990 PubMed40977347
2025
Applications
Unspecified application
Species
Unspecified reactive species
Laboratory animal research 41:21 PubMed40958130
2025
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com