Anti-Myeloperoxidase antibody [EPR20257] ab208670 is a rabbit monoclonal antibody that is used in Myeloperoxidase western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR20257 is the most widely used clone for Myeloperoxidase on the market and is cited in >240 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Myeloperoxidase staining, use in Myeloperoxidase western blotting, IHC, immunofluorescence and flow cytometry
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106131.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)")
![Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--western-blot-img40237.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)")
![Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (AB208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunocytochemistry-immunofluorescence-img135659.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124918.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)")
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--flow-cytometry-intracellular-img68388.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)")
Publications
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- Experimental animals 73:175-1852023Functional role of IL-19 in a mouse model of L-arginine-induced pancreatitis and related lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoshige Ono,Joji Horikoshi,Takeshi Izawa,Kazuhiro Nishiyama,Miyuu Tanaka,Takashi Fujita,Mitsuru Kuwamura,Yasu-Taka AzumaPubMed 38057085
- Heliyon 9:e218712023Yinhuapinggan granule ameliorates lung injury caused by multidrug-resistant inhibiting NF-κB/NLRP3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianhang Chen,Haixia Du,Huifen Zhou,Yu He,Jiehong Yang,Chang Li,Chenxing Wei,Daojun Yu,Haitong WanPubMed 38027639
- Inflammation 47:209-2262023Fibroblast Growth Factor 21 Relieves Lipopolysaccharide-Induced Acute Lung Injury by Suppressing JAK2/STAT3 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengsi Cai,Huihui Ye,Xiayan Zhu,Xiuchun Li,Luqiong Cai,Jiajia Jin,Qiwen Chen,Yuzhe Shi,Lehe Yang,Liangxing Wang,Xiaoying HuangPubMed 37864659
- Foods (Basel, Switzerland) 12:2023Inhibition of IEC-6 Cell Proliferation and the Mechanism of Ulcerative Colitis in C57BL/6 Mice by Dandelion Root Polysaccharides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengkun Yan,Lijun Yin,Rong DongPubMed 37893693
- Advanced materials (Deerfield Beach, Fla.) 35:e23058682023Treatment of Ruptured Wide-Necked Aneurysms using a Microcatheter Injectable Biomaterial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZefu Zhang,Hassan Albadawi,Richard J Fowl,Joseph L Mayer,Brian W Chong,Rahmi OkluPubMed 37579579
- Nature communications 14:61792023Gut microbiota aggravates neutrophil extracellular traps-induced pancreatic injury in hypertriglyceridemic pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanqun Li,Liwei Liu,Tianqi Lu,Yuhang Sui,Can Zhang,Yongwei Wang,Tao Zhang,Yu Xie,Peng Xiao,Zhongjie Zhao,Chundong Cheng,Jisheng Hu,Hongze Chen,Dongbo Xue,Hua Chen,Gang Wang,Rui Kong,Hongtao Tan,Xuewei Bai,Zhibo Li,Florencia McAllister,Le Li,Bei SunPubMed 37794047
- Journal of neuroinflammation 20:2222023Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihong Shi,Liang Liu,Yiyao Cao,Guangshuo Ma,Yanlin Zhu,Jianye Xu,Xu Zhang,Tuo Li,Liang Mi,Haoran Jia,Yanfeng Zhang,Xilei Liu,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Fanglian Chen,Baolong Wang,Quanjun Deng,Shu Zhang,Jianning ZhangPubMed 37777772
- Journal of nanobiotechnology 21:3542023Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zhang,Yuqi Zhou,Zhaoting Jiang,Chenhui He,Bo Wang,Qi Wang,Zeqian Wang,Tong Wu,Xiaoqi Chen,Ziwei Deng,Chunying Li,Zhe JianPubMed 37775761
- Nature communications 14:55292023Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Cortellino,V Quagliariello,G Delfanti,O Blaževitš,C Chiodoni,N Maurea,A Di Mauro,F Tatangelo,F Pisati,A Shmahala,S Lazzeri,V Spagnolo,E Visco,C Tripodo,G Casorati,P Dellabona,V D LongoPubMed 37684243
- Cell death & disease 14:5952023Gasdermin D-mediated keratinocyte pyroptosis as a key step in psoriasis pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNi Lian,Yujie Chen,Sihan Chen,Ying Zhang,Hao Chen,Yong Yang,Heng Gu,Qing Chen,Min Li,Xu ChenPubMed 37673869
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Tested |
| Rat | Expected | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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2 products for Alternative Version
Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Alternative names
Myeloperoxidase, MPO, MPO
Recommended products
Anti-Myeloperoxidase antibody [EPR20257] ab208670 is a rabbit monoclonal antibody that is used in Myeloperoxidase western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR20257 is the most widely used clone for Myeloperoxidase on the market and is cited in >240 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Myeloperoxidase staining, use in Myeloperoxidase western blotting, IHC, immunofluorescence and flow cytometry
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20257
- Purification technique
Affinity purification Protein A
- Specificity
This antibody is specific to Myeloperoxidase heavy chain.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106131.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--western-blot-img40237.jpg "Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
Negative control: HeLa PMID 12040446.
All lanes: Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution
Lane 1: Human fetal spleen lysate at 20 µg
Lane 2: Rat spleen lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 59 kDa
Exposure time: 10s
![Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunocytochemistry-immunofluorescence-img135659.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HL-60 cell line.
Negative control: HeLa (PMID: 12040446).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124918.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--flow-cytometry-intracellular-img68388.jpg "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60cells (right panel)labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ( (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730);black)andunlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa (PMID: 12040446).
![Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--western-blot-img40231.jpg "Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
All lanes: Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution
All lanes: Mouse spleen lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 59 kDa
Exposure time: 10s
![Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--western-blot-img38896.jpg "Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223 PMID: 8384653.
All lanes: Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution
All lanes: HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 39 kDa, 59 kDa, 89 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79628.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131119.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--flow-cytometry-intracellular-img126546.jpg "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730;left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
![Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--western-blot-img40231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
![Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--western-blot-img38896.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79628.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131119.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
![Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670), expandable thumbnail](https://content.abcam.com/products/images/myeloperoxidase-antibody-epr20257-ab208670--flow-cytometry-intracellular-img126546.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)")
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