AB221847

Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free

RabMAb
Recombinant
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(6 Publications)

Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF in Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Myeloperoxidase, MPO

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human spleen is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa cells (left panel) and HL-60 cells (right panel) labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and unlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : HeLa (PMID : 12040446).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HL-60 cell line.

Negative control : HeLa (PMID : 12040446).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of rat spleen is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730; left). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

Unconjugated

Anti-Myeloperoxidase antibody [EPR20257]
Biotin

Biotin Anti-Myeloperoxidase antibody [EPR20257]
HRP

HRP Anti-Myeloperoxidase antibody [EPR20257]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Myeloperoxidase antibody [EPR20257]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20257

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to Myeloperoxidase heavy chain.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Myeloperoxidase?
Anti-Myeloperoxidase [EPR20257] - BSA and Azide free (ab221847) specifically detects a band for Myeloperoxidase (UniProt: P05164) at a molecular weight of 83kDa.

Other related products
We have a range of other formats of antibody clone [EPR20257] also available for your convenience: ab208670, Carrier free - ab221847, Alexa Fluor® 488 - ab225474, ab300650, Carrier free - ab300651, Biotin - ab322301, HRP - ab323726

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0329352 PubMed40768414

2025

Quantification of H3.1-nucleosomes using a chemiluminescent immunoassay: A reliable method for neutrophil extracellular trap detection.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Wargnies,Guillaume Rommelaere,Julie Candiracci,Dorian Pamart,Robin Varsebroucq,Florian Jibassia,Finley Serneo,Virginie Laloux,Olivia Thiry,Fanny Lambert,Alison Lobbens,Priscilla Van den Ackerveken,Marielle Herzog

iScience 28:111548 PubMed39897939

2025

Neonatal infection with promotes autism-like phenotypes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eoin O'Neill,Lucy Curham,Caitlín Ní Chasaide,Síofra O'Brien,Gavin McManus,Barry Moran,Keith Rubin,Steven Glazer,Marina A Lynch,Kingston H G Mills

Cell reports. Medicine 5:101851 PubMed39657667

2024

Multi-modal analysis reveals tumor and immune features distinguishing EBV-positive and EBV-negative post-transplant lymphoproliferative disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Toh,Andrea J Reitsma,Tetsuya Tajima,Sheren F Younes,Chimere Ezeiruaku,Kayla C Jenkins,Josselyn K Peña,Shuchun Zhao,Xi Wang,Esmond Y Z Lee,Marla C Glass,Laurynas Kalesinskas,Ananthakrishnan Ganesan,Irene Liang,Joy A Pai,James T Harden,Francesco Vallania,Edward A Vizcarra,Govind Bhagat,Fiona E Craig,Steven H Swerdlow,Julie Morscio,Daan Dierickx,Thomas Tousseyn,Ansuman T Satpathy,Sheri M Krams,Yasodha Natkunam,Purvesh Khatri,Olivia M Martinez

Stem cells international 2024:2792909 PubMed39257865

2024

Astragaloside IV Treats Parkinson's Disease by Regulating the Proliferation and Differentiation of NSCs through the SHH-Nurr1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zicong Wu,Jianing Zhang,Han Gao,Wentao Li

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar

Nature cancer 2:545-562 PubMed35122017

2021

Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Sheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F Quail

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (AB221847)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Properties and storage information

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Shipped at conditions

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Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise